Multi-head integrated nucleic acid extraction test tube and method

Abstract

The invention relates to the field of nucleic acid extraction and detection, in particular to a multi-head integrated nucleic acid extraction test tube and method. The test tube comprises a main tube, an inner cavity of the main tube is sequentially provided with a cracking area, a washing area and an elution area from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking area and the washing area as well as between the washing area and the elution area for separation, and a magnetic bead washing solution and a nucleic acid eluent are respectively arranged in the washing area and the elution area; the inner cavity of the main tube is also provided with a magnetic track extending from the cracking area to the elution area; a plurality of partition plates are arranged in an inner cavity of the elution area to divide the elution area into a plurality of mutually independent branch tubes; the test tube improves the nucleic acid extraction efficiency, so that magnetic beads move more stably. Meanwhile, a plurality of independent branch tubes are arranged at the bottom end of the main tube to elute the magnetic beads respectively, so that the accuracy of an extraction result is improved.

Description

technical field [0001] The invention relates to the field of nucleic acid extraction and detection, in particular to a multi-head integrated nucleic acid extraction test tube and method. Background technique [0002] With the popularization of genetic testing, personalized drug delivery, prenatal diagnosis, etc., in today's pursuit of high-throughput and automation in various fields of the biological industry, the limitations of traditional DNA extraction methods are becoming more and more obvious. Since the extraction of nucleic acid by the magnetic bead method can realize automatic extraction and large-scale operation, and the operation is simple and the time is short, the extraction of nucleic acid by the magnetic bead method has attracted more and more attention. [0003] Chinese invention patent application (publication number CN108796038B, publication date: 20191018) discloses a nucleic acid integrated detection method and detection reagent tube, by setting a plurality...

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Problems solved by technology

[0004] The existing technology has the following disadvantages: the number of magnetic bead channels on the inner wall of the main tube is small and the width of the magnetic bead channel is narrow, and the number of magnetic beads driven by the magnet from the outer wall of the main tube at one time is small, resulting in low nucleic acid extraction efficiency; the width of the magnetic bead channel is narrow It makes the magnetic beads easy to stack on each other and cannot be evenly distributed on the inner wall of the magnetic bead channel, resulting in unstable movement of the magnetic beads

At the same time, the magnetic rod drives all the washed magnetic beads to be eluted in the same elution area at the same time, and the magnetic beads cannot be eluted separately for comparison of the results to eliminate error factors, thereby reducing the accuracy of the extraction results

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