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Application of serum amyloid A1 in preparation of biomarker for diagnosing polycystic ovarian syndrome

A technology of biomarkers and serum starch, applied in the field of biomedicine, can solve the problem of undiagnosed polycystic ovary syndrome, etc., and achieve the effect of significant technological progress

Inactive Publication Date: 2021-07-30
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
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Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides the use of serum amyloid A1 in the preparation of a biomarker for the diagnosis of polycystic ovary syndrome. Technical aspects of the method for diagnosing polycystic ovary syndrome

Method used

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  • Application of serum amyloid A1 in preparation of biomarker for diagnosing polycystic ovarian syndrome
  • Application of serum amyloid A1 in preparation of biomarker for diagnosing polycystic ovarian syndrome
  • Application of serum amyloid A1 in preparation of biomarker for diagnosing polycystic ovarian syndrome

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Experimental program
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Embodiment 1

[0017] 【Experimental Technology】:

[0018] 1. Experimental methods and materials

[0019] 1. Collection of human serum and follicular fluid: collect serum and follicular fluid from PCOS and control patients.

[0020] The above cases were from the Reproductive Medicine Center of Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine, and all of them were reviewed by the Ethics Committee of Renji Hospital.

[0021] The diagnostic criteria for PCOS were the Rotterdam criteria, and the controls were patients with pure tubal factor infertility or male factor infertility. Ovulation induction was performed using conventional methods, and blood and follicular fluid were collected on the day of egg retrieval. Serum was obtained after blood centrifugation, the first tube of dominant follicular fluid was centrifuged and then frozen, and the remaining follicular fluid was density gradient centrifuged and enzyme digested to obtain granulosa cells for cryopreservatio...

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Abstract

The invention provides application of serum amyloid A1 in preparation of a biomarker for diagnosing polycystic ovarian syndrome. The invention further provides application of the reagent for detecting the serum amyloid A1 in preparation of a diagnostic composition in a biomarker for in-vitro diagnosis of the polycystic ovarian syndrome, the sequence of an upstream primer for detecting the serum amyloid A1 is as shown in SEQ ID NO: 2, and the sequence of a downstream primer for detecting the serum amyloid A1 is as shown in SEQ ID NO: 3. The diagnosis comprises the steps: detecting the level of the serum amyloid A1 biomarker from a patient. Experimental research results show that SAA1 plays an important role in occurrence and development of the PCOS disease, and SAA1 can be used as a biomarker for evaluating the state of the PCOS disease.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to serum amyloid A1, specifically the use of serum amyloid A1 as a biomarker in the diagnosis of polycystic ovary syndrome. Background technique [0002] Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disorder and metabolic abnormality in women of childbearing age. About 75%. PCOS is characterized by oligoovulation or anovulation, hyperandrogenism and / or clinical hyperandrogenism, polycystic ovaries as the main features, and typical clinical manifestations as infertility, obesity, impaired glucose tolerance and insulin resistance. So far, the etiology and pathogenesis of PCOS are still unclear. [0003] Serum amyloid A (SAA) is a reactive protein in the acute phase of inflammation or injury. It is mainly secreted by the liver in a short period of time, exceeding 1000 times the normal value. It is currently one of the most sensitive inflammatory response markers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158
Inventor 孙贇
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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