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Bacillus siamensis Gxun-36 capable of secreting siderophore and application thereof

A technology of gxun-36 and Bacillus, applied to Bacillus siamese Gxun-36 and its application field

Active Publication Date: 2021-08-03
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, some strains that can not only secrete siderophores but also prevent and treat pathogenic bacteria have been screened, but there are no reports that can secrete siderophores and have a good control effect on banana wilt caused by Fusarium oxysporum. Bacillus

Method used

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  • Bacillus siamensis Gxun-36 capable of secreting siderophore and application thereof
  • Bacillus siamensis Gxun-36 capable of secreting siderophore and application thereof
  • Bacillus siamensis Gxun-36 capable of secreting siderophore and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Isolation and identification of embodiment 1 bacterial strain

[0038] The soil collected in the banana rhizosphere of the Guangxi Jinsui Group farm was diluted by 10 -1 、10 -2 、10 -3 For three gradients, 100 μL of each concentration was drawn and spread on PDA, LB, and NA plate medium to separate single colonies. The isolated strains were spotted on the CAS double-layer plate medium, and cultured at 30°C for 48 hours. According to the color change on the plate and the diameter of the aperture, the strains with strong siderophore secretion were screened out.

[0039] Using Fusarium oxysporum race No. 4 as indicator bacteria, the antagonistic activity screening of isolated and screened strains was carried out. First, Fusarium oxysporum was inoculated on the PDA plate medium, and cultured at 30°C for 7 days. Then use a sterilized hole puncher to make a 5mm round bacterial cake, inoculate it into the center of the PDA plate medium again, and connect the bacterial liqui...

Embodiment 2

[0056] Three leaf-age banana seedlings were transplanted into flower pots with sterilized soil and vermiculite as the substrate. After 10 days of transplanting and colonization, banana seedlings with basically the same growth were selected and grouped into two groups. For the control group and the treatment group, each group had 3 repetitions. After the banana seedlings in the two groups were treated with root injury, the roots of the control group were inoculated with 20 mL of the spore suspension of Fusarium oxysporum No. Seed spore suspension + 20mL Gxun-36 fermentation broth. After 7 days of root irrigation treatment, the control group and the treatment group were again irrigated with 20 mL of sterile water and Gxun-36 fermentation broth. 30 days after the first root irrigation and inoculation, observe and record the incidence of banana seedlings, and calculate the incidence index and control effect of each group. The result is as Figure 5 shown.

[0057] The incidenc...

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Abstract

The invention relates to the technical field of microorganisms, in particular to Bacillus siamensis Gxun-36 capable of secreting siderophores and application thereof. The Bacillus siamensis Gxun-36 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, the address of the Guangdong Microbial Culture Collection Center is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, the preservation date is April 23, 2021, and the preservation number is GDMCC No: 61616. The strain provided by the invention can secrete siderophores, can inhibit phytopathogens, and also has a good control effect on banana wilt caused by fusarium oxysporum.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a siderophore-secreting bacillus siamese Gxun-36 and its application. Background technique [0002] Fusarium wilt of banana is the most serious fungal soil-borne disease caused by Fusarium oxysporum f.sp.cubense (FOC4). Banana wilt has been called "banana cancer" or "banana AIDS." At present, the methods commonly used in agricultural production to prevent and control banana Fusarium wilt include chemical control, breeding of disease-resistant varieties and biological control. Although chemical control can achieve certain control effects in the short term, due to the fact that the disease is a soil-borne disease, effective chemical agents have not been found for continuous control, and long-term application of pesticides may lead to the development of resistance of pathogenic bacteria, thereby affect crop yield and quality. Breeding disease-resistant varieties is theoretically...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22
Inventor 申乃坤姜明国李枢妍张红岩王一兵朱英芝丰景
Owner GUANGXI UNIV FOR NATITIES