Composition of albendazole and multiple myeloma clinical medicine and application
A multiple myeloma, albendazole technology, applied in the field of medicine, to achieve a strong inhibitory effect
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Embodiment 1
[0026] In this embodiment, albendazole of different concentrations is used to treat ANBL6-BR cells, and the survival rate is detected by manual counting. The influence of ANBL6-BR cell clone formation ability, the experimental steps are as follows:
[0027] 1. ANBL6-BR comes from the Institute of Hematology, Chinese Academy of Medical Sciences, and is a MM cell resistant to BTZ. The sensitivity of ANBL6-BR cells to BTZ was detected by manual counting; ANBL6-BR cells were treated with different concentrations of albendazole, and the effect of albendazole on the survival rate of ANBL6-BR cells was calculated by manual counting;
[0028] ① Collect the ANBL6-BR cells in the logarithmic growth phase, resuspend in Sijiqing 1640 medium (Gibco) containing 20% FBS (fetal bovine serum, Sijiqing) in mass concentration, add to 96-well plate, make each well 1× 10 4 cells, the volume of each well is 100 μL;
[0029] ②A total of 8 groups were set up, which were the control group with dr...
Embodiment 2
[0039] In this example, the method of cell counting was used to detect the synergistic effect of albendazole and BTZ combined with different concentration ratios on ANBL6-BR cells. The experimental steps are as follows:
[0040] 1. Collect ANBL6-BR cells in the logarithmic growth phase, adjust the concentration of the cell suspension, add to a 96-well plate, 100 μL per well, 1×10 4 cells;
[0041]2. Set up the control group that adds drug solvent (1640 medium that contains volume concentration 0.1% DMSO as drug solvent), experimental group adds independent ABZ (0.05 μ M, 0.1 μ M, 0.2 μ M), independent BTZ (10nM, 20nM , 40nM) and ABZ combined with BTZ (0.05μM+20nM, 0.05μM+40nM, 0.1μM+10nM, 0.1μM+20nM, 0.1μM+40nM, 0.2μM+10nM, 0.2μM+20nM, 0.2μM+40nM) , 15 groups in total, set 3-5 replicate wells for each concentration, and incubate in a cell culture incubator for 48 hours;
[0042] 3. Mix the cells in the well plate, mix the cell suspension with the mass concentration of 0.4% ...
Embodiment 3
[0045] In this example, flow cytometry was used to detect ALDH1 after albendazole and BTZ alone or in combination treated MM cells resistant to BTZ + Changes in cell proportions. Aldehyde dehydrogenases (ALDHs) are detoxifying enzymes highly expressed in various cancer stem cells (CSCs) including multiple myeloma stem-like cells (MMSCs). ALDH1 + MM cells represent a part of MMSCs, or from a certain point of view, represent a more extensively differentiated proliferating progenitor cell population, which has stem cell characteristics. Detection of ALDH1 + The ALDEFLUOR kit of cell number ratio was purchased from Stem Cell Technologies, and the ALDH1 + For the detection of cells, PI is propidium iodide, which is a nucleic acid dye.
[0046] 1. Flow cytometry detection of ALDH1 in ANBL6-BR cells treated with albendazole and BTZ alone or in combination + Changes in cell ratio;
[0047] ① Collect ANBL6-BR cells in the logarithmic growth phase, adjust the concentration of the ...
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