Rice calmodulin B protein and application thereof in breeding

Abstract

The invention discloses a rice calmodulin B protein and application thereof in breeding, and relates to the technical field of agricultural breeding. The breeding comprises the step of creating germplasm with low germination rate of gramineous plant seeds and germplasm with relatively low seedling height, relatively small root quantity and relatively short length through a transgenic technology. The gramineous plants comprise rice. The rice calcium phosphatase B protein is CBL8; the overexpression plants L8-OE5 and L8-OE6 of the CBL8 both show a phenotype for inhibiting the seed germination of the plant and a phenotype for inhibiting the seedling growth of the plant; the CBL8-deleted mutants CRISPRL8-48 and CRISPRL8-51 have the same phenotype as the wild phenotype, the seed germination and seedling growth are normal, and the CBL8-deleted mutants CRISPRL8-48 and CRISPRL8-51 have the same phenotype as the wild phenotype. The research can provide a theoretical basis for transgenic breeding of gramineous plants.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a rice-like calcineurin B protein and its application in breeding. Background technique [0002] Oryza genus belongs to the door of angiosperms, the class of unit cotyledons, the family of Poaceae, and the genus Oryza. Annual aquatic herb, 0.5-1.5m high. The leaves are alternate in two rows, linear-lanceolate. Spikes are panicles, self-pollinating. The advantage of rice is that it grows fast, can produce one or even two generations a year, has many grains, and has strong vitality. The genome sequence of rice indica and japonica subtypes has been published, and the functional genes account for a high proportion of the total genome, such as the indica type, which accounts for 92%. The functions of many genes are still unknown, so it is particularly necessary to clone its related genes and study their functions . [0003] Rice research includes forward genetics an...

AI Technical Summary

Problems solved by technology

[0004] In view of the advantages of rice in genetic manipulation, it is widely used in the research of many processes of plant life activities, and a series of important discoveries have been made; but for the research of rice-like calcineurin B protein and its effect on rice seed germination and The effect of seedling growth needs to be further studied and applied to the cultivation of rice to provide a theoretical basis for future breeding work

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