Azo high polymer for transporting medicine to intestinal tracts and releasing medicine as well as preparation method and application of azo high polymer
A polymer and azo technology, applied in the field of medicine, can solve problems such as reducing transportation efficiency
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Embodiment 1
[0034] The synthetic method of embodiment 1 azo high polymer polymer-DE is as follows:
[0035]① Add 4-nitroaniline (2.0 g, 14.48 mmol) into ultrapure water (15 mL), and add concentrated HCl (3.7 mL). Stir at room temperature for 30 minutes at 600 rpm. The solution was then cooled to 0~5°C, and a solution of sodium nitrite (1.0 g, 14.49 mmol) in water (15 mL) was added slowly. The resulting mixture was stirred at 0~5°C for 1 hour, then added to a solution of 3-hydroxybenzoic acid (2.0 g, 14.48 mmol) and NaOH (1.16 g, 28.96 mmol). Add dropwise (15 mL) via addition funnel at 0°C. The resulting suspension was stirred for another hour. The orange precipitate was then filtered and washed well with water. After vacuum freeze-drying, an orange solid compound A (3.3363 g, 11.62 mmol) was obtained with a yield of 80.22%.
[0036] Detection shows that the prepared compound A nuclear magnetic characterization is as follows: 1 H NMR (400MHz, CD 3 OD) δ8.38(d, J=9.1Hz, 2H), 7.99(d, ...
Embodiment 2
[0049] Degradation experiment of embodiment 2 azo high polymer polymer-D'1E'1:
[0050] ①Cultivate Escherichia coli BL21 in 100mL LB liquid medium to OD 600nm After =0.6~0.8, centrifuge for 5min at a speed of 8000rpm, and remove the supernatant;
[0051] ②Add 7.5mL buffer solution (Tris(20mM), NaCl(300mM),pH=7.00), freeze-thaw and ultrasonically lyse 6 times, then centrifuge at 8000rpm for 5min each time, and keep the supernatant;
[0052] ③ Add 75 μL each of kanamycin (5 mg / mL) and ampicillin sodium (5 mg / mL), 300 μL of NADH (15 mM), and 75 μL of polymer-D’1E’1 in DMSO (5 mg / mL). Incubate for 14 hours in a shaker at 37°C with a rotation speed of 180 rpm;
[0053] ④Centrifuge the cultured mixed solution for 5min at 8000rpm, remove the supernatant, add 7.5mL DMSO to the precipitate to completely dissolve polymer-D'1E'1;
[0054] ⑤Centrifuge again, measure the ultraviolet absorbance of the supernatant, and compare it with the DMSO solution of polymer-D'1E'1 with the same init...
Embodiment 3
[0056] The degradation experiment of embodiment 3 azo high polymer polymer-D'3E'1:
[0057] ①Cultivate Escherichia coli BL21 in 100mL LB liquid medium to OD 600nmAfter =0.6~0.8, centrifuge for 5min at a speed of 8000rpm, and remove the supernatant;
[0058] ②Add 7.5mL buffer solution (Tris(20mM), NaCl(300mM),pH=7.00), freeze-thaw and ultrasonically lyse 6 times, then centrifuge at 8000rpm for 5min each time, and keep the supernatant;
[0059] ③ Add 75 μL each of kanamycin (5 mg / mL) and ampicillin sodium (5 mg / mL), 300 μL of NADH (15 mM), and 75 μL of polymer-D’3E’1 in DMSO (5 mg / mL). Incubate for 14 hours in a shaker at 37°C with a rotation speed of 180 rpm;
[0060] ④Centrifuge the cultured mixed solution for 5min at 8000rpm, remove the supernatant, add 7.5mL DMSO to the precipitate to completely dissolve polymer-D'3E'1;
[0061] ⑤Centrifuge again, measure the ultraviolet absorbance of the supernatant, and compare it with the DMSO solution of the same initial concentration...
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