Preparation method for circular single-stranded DNA integrated with aptamer and applications of circular single-stranded DNA integrated with aptamer in DNA origami

A nucleic acid aptamer and aptamer technology, applied in the fields of DNA nanotechnology and biological applications, can solve the problems of low ligation efficiency and stability, and achieve the effects of efficient preparation and rich diversity
CN113278607APending Publication Date: 2021-08-20NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV
Publication Date
2021-08-20

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Abstract

The invention discloses a preparation method for circular single-stranded DNA integrated with aptamer and applications of the circular single-stranded DNA integrated with aptamer in DNA origami, and belongs to the field of DNA nanotechnologies and biological application. The preparation method mainly includes the following steps: (1.1) constructing circular double-stranded recombinant phagemid including an aptamer sequence; and (1.2) converting the constructed circular double-stranded recombinant phagemid into competent escherichia coli cells; and extracting the circular single-stranded DNA integrated with aptamer. Through the preparation method, a user defined skeleton chain sequence can be designed and produced, so as to add various function sequences at a user defined position. Problems of poor efficiency and stability of structural functionalization of traditional DNA origami can be broken through.
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Description

technical field

[0001] The invention belongs to the field of DNA nanotechnology and biological applications, in particular to a method for preparing circular single-stranded DNA integrated with nucleic acid aptamers and its application in DNA origami; in particular, it relates to the preparation of different nucleic acid aptamers Functionalized single-stranded DNA (single-stranded DNA, ssDNA) is used as the backbone strand of DNA origami to construct functional DNA origami structures. Background technique

[0002] As a natural biomacromolecule, DNA has unique advantages in the construction of functional nanostructures due to its unique chemical structure and unique intermolecular interactions; DNA molecules can self-assemble to form nanostructures with complementary base pairing. Ordered structure with nanoscale precision; this DNA nanostructure can also modify the DNA chain at a specific position, making it a scaffold to guide other molecules or nanomaterials to perform con...

Claims

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