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A kind of ephedra extract and preparation method thereof

A technology of extract and ephedra, applied in the field of ephedra extract and preparation thereof, can solve the problems such as not considering the conversion of methylephedrine hydrochloride, and achieve the effects of increasing the content of methylephedrine hydrochloride, promoting fermentation effect, and improving conversion rate

Active Publication Date: 2022-04-08
HAINAN HULUWA PHARMA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN112220808A discloses a kind of ephedra extract and its active component and application, and described ephedra extract is ephedra water extract and ephedra ethanol extract, and described active component comprises gossythin, gallic acid, quercetin and apigenin, This method is not suitable for extracting ephedrine, etc.
This purification process is mainly aimed at the purification of the total alkaloids of ephedra, without considering the conversion of methylephedrine hydrochloride, etc.

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  • A kind of ephedra extract and preparation method thereof
  • A kind of ephedra extract and preparation method thereof

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Embodiment 1

[0025] The preparation method of embodiment 1 ephedra extract

[0026] (1) Fermentation: get the ephedra Chinese medicinal material and crush, grind, and sieve to obtain ephedra powder, add a volume fraction of 60% ethanol solution, and a mass fraction of 0.35% sodium hydrogen phosphate solution, wherein ethanol solution, sodium hydrogen phosphate solution and ephedra The mass ratio of powder is 1.25:0.4:1, the pH value is adjusted to 4.7, and then Saccharomyces cerevisiae powder is added. The amount of Saccharomyces cerevisiae powder added is 7% of the mass of ephedra powder, and fermented at 28-30°C for 10 hours to obtain fermentation material;

[0027] (2) Extraction: extract the fermented material in subcritical water at 138°C for 12 minutes, the mass-volume ratio of fermented material and water kg / L is 1:25, and obtain the extract, which is centrifuged at 5500r / min for 4min, Take the supernatant and set aside;

[0028] (3) Purification: the supernatant is loaded into a ...

Embodiment 2

[0030] The preparation method of embodiment 2 ephedra extract

[0031] (1) Fermentation: get the ephedra Chinese medicinal material and crush, grind, and sieve to obtain ephedra powder, add a volume fraction of 55% ethanol solution, and a mass fraction of 0.5% sodium hydrogen phosphate solution, wherein ethanol solution, sodium hydrogen phosphate solution and ephedra The mass ratio of powder is 1.2:0.5:1, the pH value is adjusted to 4.6, and then Saccharomyces cerevisiae powder is added. The amount of Saccharomyces cerevisiae powder added is 5% of the mass of ephedra powder, and fermented at 28-30°C for 8 hours to obtain fermentation. material;

[0032] (2) Extraction: extract the fermented material in subcritical water at 135°C for 15 minutes, the mass-volume ratio of fermented material and water kg / L is 1:20, to obtain the extract, and centrifuge the extract at 5000r / min for 5min, Take the supernatant and set aside;

[0033] (3) Purification: the supernatant is loaded into...

Embodiment 3

[0035] The preparation method of embodiment 3 ephedra extract

[0036] (1) Fermentation: get the ephedra Chinese medicinal material and crush, grind, and sieve to obtain ephedra powder, add a volume fraction of 65% ethanol solution, and a mass fraction of 0.3% sodium hydrogen phosphate solution, wherein ethanol solution, sodium hydrogen phosphate solution and ephedra The mass ratio of powder is 1.3:0.3:1, the pH value is adjusted to 4.8, and then Saccharomyces cerevisiae powder is added. The amount of Saccharomyces cerevisiae powder added is 8% of the mass of ephedra powder, and fermented at 28-30°C for 12 hours to obtain fermented material;

[0037] (2) Extraction: extract the fermented material in subcritical water at 142°C for 8 minutes, the mass-volume ratio of fermented material and water kg / L is 1:30, to obtain the extract, and centrifuge the extract at 6000r / min for 3min, Take the supernatant and set aside;

[0038] (3) Purification: the supernatant is loaded into a r...

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Abstract

The invention provides an ephedra extract and a preparation method thereof, comprising the following steps: (1) crushing the ephedra Chinese medicinal material, grinding into powder, and sieving to obtain ephedra powder, adding ethanol solution with a volume fraction of 55%-65%, and a mass fraction of 0.3% to 0.5% sodium hydrogen phosphate solution, adjust the pH value to 4.6 to 4.8, then add Saccharomyces cerevisiae powder, and place it at 28 to 30°C to ferment for 8 to 12 hours to obtain a fermented material; (2) put the fermented material in Extract with subcritical water at 135-142°C for 8-15 minutes, centrifuge, and take the supernatant; (3) Load the supernatant into a reverse-phase chromatographic column for gradient elution and separation, using a mixed solvent of methanol and water as the elution (4) The purified liquid is spray-dried, the feed flow rate is 190-210mL / h, the inlet air temperature is 280-320°C, after drying, it is sieved to obtain the target ephedra extract and improve the efficacy At the same time, it is beneficial to reduce adverse reactions.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to an ephedra extract and a preparation method thereof. Background technique [0002] Ephedra is the dry herbaceous stem of Ephedra sinica Stapf, a plant in the Ephedra family. Ephedra mainly contains alkaloids, flavonoids, organic acids, etc., wherein the active ingredient of alkaloids is mainly ephedrine, and ephedrine includes ephedrine hydrochloride, pseudoephedrine, and methylephedrine hydrochloride. Among them, the antiasthmatic effect of methylephedrine hydrochloride is greater than that of ephedrine and pseudoephedrine, and it is safer. The extraction rates of water and ethanol extraction are higher. CN112220808A discloses a kind of ephedra extract and its active ingredient and application, and described ephedra extract is ephedra water extract and ephedra ethanol extract, and described active ingredient comprises gossythin, gallic acid, quercetin and apigenin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/17A61P11/14A61K135/00
CPCA61K36/17A61P11/14A61K2236/333A61K2236/19A61K2236/37A61K2236/53A61K2236/55A61K2236/51
Inventor 刘景萍刘全国陈克领吴伟贞
Owner HAINAN HULUWA PHARMA GRP CO LTD
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