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Immunochromatography device for detecting SARS-CoV-2 virus neutralizing antibody and application thereof

An immunochromatography, sars-cov-2 technology, applied in the field of antibody detection, can solve the problems of inability to distinguish between neutralizing antibodies and non-neutralizing antibodies, and inability to accurately evaluate the effect of immune protection.

Active Publication Date: 2021-08-24
NANJING GENSCRIPT BIOTECH CO LTD
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Problems solved by technology

SARS-CoV-2 serum detection methods such as chemiluminescence, enzyme-linked immunosorbent assay (ELISA), and colloidal gold detection methods all analyze SARS-CoV-2 total antibody (Ab) and SARS-CoV-2 IgM antibody, but Unable to distinguish SARS-CoV-2 neutralizing antibodies from non-neutralizing antibodies, unable to accurately evaluate the immune protection effect

Method used

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  • Immunochromatography device for detecting SARS-CoV-2 virus neutralizing antibody and application thereof
  • Immunochromatography device for detecting SARS-CoV-2 virus neutralizing antibody and application thereof
  • Immunochromatography device for detecting SARS-CoV-2 virus neutralizing antibody and application thereof

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Embodiment Construction

[0066] The principle of the present invention can be expressed as: a method for detecting SARS-CoV-2 in samples by blocking the combination of SARS-CoV-2 virus RBD protein and ACE2 receptor by immunochromatography and SARS-CoV-2 virus neutralizing antibody. CoV-2 virus neutralizing antibodies. It immobilizes the recombinant ACE2 protein on the detection membrane. When the SARS-CoV-2 virus neutralizing antibody exists in the detection sample, the neutralizing antibody binds to the SARS-CoV-2 virus RBD protein labeled by the signal object and blocks the recombinant ACE2 protein on the membrane from the SARS-CoV-2 labeled by the signal object. 2. When the virus RBD protein is combined, no detection signal (or band) appears, and the detection result is positive. When there is no SARS-CoV-2 virus neutralizing antibody in the test sample, the recombinant ACE2 receptor on the membrane binds to the signal-labeled SARS-CoV-2 virus RBD protein. A detection signal (or band) appears and...

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Abstract

The invention relates to the field of antibody detection, in particular to an immunochromatography device for detecting an SARS-CoV-2 virus neutralizing antibody and application of the immunochromatography device, and further relates to a preparation method and a use method of the immunochromatography device for detecting the SARS-CoV-2 virus neutralizing antibody. The immunodetection device for detecting the coronavirus neutralizing antibody comprises a coronavirus spike protein part, ACE2 protein or a functional fragment thereof specifically combined with the coronavirus spike protein part and a solid-phase support, By detecting influence of a sample to be detected on the combination of the coronavirus spike protein part and the ACE2 protein or the functional fragment thereof on the solid-phase support, whether the coronavirus neutralizing antibody exists in the sample or not is judged. The immunochromatography device disclosed by the invention has specific, sensitive, rapid, simple and convenient detecting test paper, and is easy to popularize and apply in production practice.

Description

technical field [0001] The invention relates to the field of antibody detection, in particular to a method for detecting SARS-CoV-2 virus neutralizing antibodies by immunochromatography technology and an immunochromatography detection device for SARS-CoV-2 virus neutralizing antibodies. Background technique [0002] The novel coronavirus pneumonia (COVID-19) pathogen SARS-CoV-2 virus, also known as 2019 novel coronavirus (2019 Novel coronavirus, 2019-nCoV), is an RNA coronavirus. At present, more than 4.7 million confirmed cases have been reported in more than 220 countries and regions around the world, and more than 300,000 patients have died. The COVID-19 outbreak has gradually turned into a global pandemic. Vaccines and specific drugs for SARS-CoV-2 virus disease have not yet appeared in the world. [0003] The SARS-CoV-2 virus is a class of RNA viruses with an envelope and a linear single-stranded positive strand genome. SARS-CoV-2 virus entry into host cells is media...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/558G01N33/54313G01N33/54346G01N2333/165G01N2469/20
Inventor 覃喜建虎慧珍
Owner NANJING GENSCRIPT BIOTECH CO LTD
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