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Preparation method and application of human mesenchymal stem cell supernatant dry powder gel

A stem cell and stem cell technology, applied in the field of preparation of human mesenchymal stem cell supernatant dry powder gel, can solve problems such as hindering popularization and application, short storage time, etc. Effect

Pending Publication Date: 2021-08-27
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The storage time of liquid mesenchymal stem cell culture supernatant at room temperature is short, which hinders its promotion and application

Method used

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  • Preparation method and application of human mesenchymal stem cell supernatant dry powder gel
  • Preparation method and application of human mesenchymal stem cell supernatant dry powder gel

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Experimental program
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Embodiment Construction

[0033] The preparation method of human mesenchymal stem cell supernatant dry powder gel comprises the following operations:

[0034] 1) Cultivation of mesenchymal stem cells: human umbilical cord mesenchymal stem cells were cultured and passaged in complete α-MEM medium.

[0035] 2) Collection of mesenchymal stem cell supernatant: collect low-passage human mesenchymal stem cell culture supernatant; when the cell growth density reaches 80-90%, collect the culture supernatant, sterile centrifuge at 1200rpm for 10min, and discard the precipitate Take the supernatant, and freeze the supernatant at -80°C for later use;

[0036] 3) Preparation of freeze-dried powder: put the freeze-dried solid at -80°C in a freeze dryer with a vacuum pressure of 0.030 mbar, and evaporate water at -88°C for 48 hours to obtain a dry powder. Finally, freeze-dried powder was prepared, weighed, wrapped in tin foil, registered, and stored at -80°C.

[0037] 4) Preparation of freeze-dried powder gel

[...

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Abstract

The invention discloses a preparation method and application of human mesenchymal stem cell supernatant dry powder gel. According to the invention, serum-free mesenchymal stem cell complete culture solution is used for culture and passage of human umbilical cord mesenchymal stem cells, and 3-5 generations of mesenchymal stem cell supernatant with low passage is collected to prepare freeze-dried powder. According to the preparation method of the freeze-dried powder of the human umbilical cord mesenchymal stem cell culture supernatant, the prepared freeze-dried powder effectively preserves a mixture of various cell factors with biological activity in the human umbilical cord mesenchymal stem cell supernatant, and can effectively solve the problems that the storage life of the supernatant is limited; and a foundation is laid for clinical and industrial application of the human mesenchymal stem cell culture solution.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and relates to a preparation method and application of human mesenchymal stem cell supernatant dry powder gel. Background technique [0002] The skin is the largest endocrine and immune organ of the human body, and constitutes the body's first barrier against external adverse factors. Due to direct contact with the external environment, it is extremely vulnerable to damage by various physical, chemical, pathogenic microorganisms, or surgery. The incidence of skin trauma is high, the repair process is complicated, and it is easy to cause problems such as poor healing, resulting in huge economic and social burdens, and seriously affecting the quality of life of patients. Clinically, debridement, anti-inflammation and other treatment methods are routinely used for skin trauma to resist infection and promote wound healing, and antibiotics are often used to assist anti-inflammation to heal naturall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K35/28A61K47/10A61K47/22A61K47/32A61P17/02C12N5/0775
CPCA61K9/06A61K35/28A61K47/10A61K47/22A61K47/32A61P17/02C12N5/0668
Inventor 华进联艾力·艾尔肯
Owner NORTHWEST A & F UNIV
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