Method for rapidly detecting impedance of escherichia coli in food based on nano magnetic beads
A technology of Escherichia coli and nano magnetic beads, which is applied in the direction of nanotechnology, nanotechnology, nanotechnology, etc. for materials and surface science, can solve the problems of complex pretreatment steps, expensive instruments, etc., and achieve flexible structure, rapid detection, The result is a stable effect
Pending Publication Date: 2021-08-27
NINGBO HAITONG FOOD SCI & TECH CO LTD +1
View PDF7 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
These techniques are highly sensitive but require expensive instrumentation, complex preprocessing steps and specialized operators
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0031] A kind of impedance rapid detection method of Escherichia coli in food based on nano-magnetic beads of the present invention comprises the following steps:
[0032] The first step is to obtain a graphene paper flexible electrode by means of vacuum filtration and reduction, and modify the surface of the electrode with nanoparticles and polymers to obtain a graphene / nanocomposite paper electrode;
[0033] The second step is to modify the surface of the graphene / nano-composite paper electrode with elements with the recognition function of E. coli;
[0034] The third step is to synthesize the corresponding nano-magnetic beads and modify the recognition elements with the ability to capture E. coli on the surface of the magnetic beads;
[0035] The fourth step, the immunomagnetic beads capture the bacteria and combine with the antibody on the electrode surface to form a sandwich structure and generate an impedance signal;
[0036] The fifth step is to analyze the linear rela...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention belongs to the field of food safety detection, and relates to a method for rapidly detecting impedance of escherichia coli in a food based on nano magnetic beads, which comprises the following steps: 1, obtaining a graphene paper flexible electrode through vacuum filtration and reduction, and modifying the surface of the electrode with nano gold particles; 2, modifying the surface of a graphene / nanogold composite paper electrode with an element with an escherichia coli recognition function; 3, synthesizing corresponding nano magnetic beads, and modifying the surfaces of the magnetic beads with recognition elements with escherichia coli capturing capability; 4, combinng the immunomagnetic beads capture bacteria with an antibody on the surface of the electrode to form a sandwich structure, and generating impedance signals. An impedance biosensor prepared through the method is higher in detection sensitivity, more stable in detection result and wider in detection range, and has wide application prospects, and huge economic benefits can be obtained.
Description
technical field [0001] The invention discloses a method for quickly detecting the impedance of Escherichia coli in food based on nano magnetic beads, and belongs to the technical field of food safety detection. Background technique [0002] Pathogenic bacteria that can cause infectious diseases are increasingly attracting public attention. At the same time, the infection of pathogenic bacteria will also cause huge economic losses, and it has also sounded the alarm bell of food safety to the world. Among the many pathogens that can cause life-threatening symptoms, enterohemorrhagic E. coli can cause very serious outbreaks of foodborne illness. [0003] At present, new requirements are put forward for the detection methods of pathogenic bacteria, such as providing rich information of pathogenic bacteria simply, quickly and sensitively, especially in areas with limited resources. A large number of complex methods, such as polymerase chain reaction (PCR) method, DNA microarray...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N27/12G01N27/26G01N27/327G01N33/543G01N33/569B82Y30/00C12Q1/10
CPCG01N27/127G01N27/327G01N27/26G01N33/54326G01N33/54346G01N33/5438G01N33/56916C12Q1/10B82Y30/00
Inventor 樊凯陈移平王剑平陈晶晶
Owner NINGBO HAITONG FOOD SCI & TECH CO LTD