Application of nucleoside hydrolase 21 in preparation of reagents for detection and/or treatment of multiple myeloma
A technology for multiple myeloma and nucleoside water, which is applied in the application field of nucleoside hydrolase 21 in the preparation of reagents for detection and/or treatment of multiple myeloma, which can solve the problem of limiting long-term curative effect, lack of research on drug resistance mechanisms, Problems such as solutions did not appear
Active Publication Date: 2022-06-28
ACADEMY OF MILITARY MEDICAL SCI +1
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Problems solved by technology
Although the bortezomib-based chemotherapy regimen can significantly improve the symptoms of MM patients and delay tumor progression in the short term, almost all MM patients who use bortezomib will develop drug resistance characteristics after a period of medication, which is of great significance. Limits the long-term efficacy of the drug for MM
So far, there is still little research on the mechanism of drug resistance of MM to bortezomib, the mechanism of drug resistance is still unclear, and there is no effective clinical solution
Method used
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Embodiment 1
[0040] Real-Time Reverse Transcript Polymerase Chain Reaction (RT-PCR)
[0041] Total RNA was extracted from RPMI-8226 and SKO-007 cells with Trio Reagent (Invitrogen, Carlsbad, CA, USA). Specific conditions and parameters According to the protocol in the kit instructions, 2 μg of total RNA was reversed into cDNA using TransScript One-StepgDNA Removal and cDNA Synthesis SuperMix (Beijing Quanshi Gold, China) according to the instructions. By using SYBR-Green I as a double-stranded DNA specific binding dye, real-time PCR was performed in a 7500 detection system, and the system and procedures were performed according to the protocol of the Genestar kit. The mRNA of the target gene and the mRNA of the housekeeping gene β-actin were quantified in separate tubes.
[0042] The primers used for RT-PCR are shown in Table 1.
[0043] Table 1 RT-PCR primer sequences
[0044]
[0045] RNA-Seq
[0046] 1 μg of RPMI-8226 or SKO-007 cell-derived total RNA was prepared for cDNA librar...
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The invention provides the application of nucleoside hydrolase 21 in the preparation of reagents for detection and / or treatment of multiple myeloma, which belongs to the technical field of detection and treatment of multiple myeloma. The invention comprehensively analyzes Broad Institute Cancer Cell Line Encyclopedia data sets and The research results of the present invention found that NUDT21 is highly expressed in MM cells, including MM patient CD138+ cells and MM cell lines RPMI‑8226, SKO‑007, U266 and XG7. RPMI‑8226 cells and SKO‑007 cells suppressed the expression level of NUDT21 after bortezomib treatment. Silencing NUDT21 expression can significantly inhibit the proliferation of RPMI-8226 cells and SKO-007 cells (including the ability of cell number expansion and colony formation in liquid medium culture state).
Description
technical field [0001] The invention belongs to the technical field of detection and treatment of multiple myeloma, and particularly relates to the application of nucleoside hydrolase 21 in the preparation of reagents for detection and / or treatment of multiple myeloma. Background technique [0002] Multiple myeloma (MM) is a hematological malignancy originating from the lymphoplasmacytic cell lineage. Its typical feature is abnormal proliferation of plasma cells, resulting in increased blood calcium, renal dysfunction, anemia, bone disease and other clinical manifestations. The median age at diagnosis of MM was 69 years, and the median overall survival was 6 to 7 years. Chemotherapy is the main treatment for MM. In recent years, with the in-depth exploration of the pathogenesis of MM, researchers have screened out targeted therapy drugs such as proteasome inhibitors and immunomodulators at the molecular level. The emergence of new drugs has made the treatment of MM more eff...
Claims
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IPC IPC(8): C12Q1/6886G01N33/574G01N33/573A61K45/00A61K38/05A61P35/00
CPCC12Q1/6886G01N33/57407G01N33/57484G01N33/573A61K45/00A61K38/05A61P35/00C12Q2600/158
Inventor 肖凤君王立生高川成孙慧燕
Owner ACADEMY OF MILITARY MEDICAL SCI