Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of nucleoside hydrolase 21 in preparation of reagent for detecting and/or treating multiple myeloma

A technology for multiple myeloma and nucleoside water, which is applied in the application field of nucleoside hydrolase 21 in the preparation of reagents for detection and/or treatment of multiple myeloma, and can solve the problem that there are few researches on the drug resistance mechanism, and the drug resistance mechanism is unclear. Issues such as clarity, no solutions, etc.

Active Publication Date: 2021-08-31
ACADEMY OF MILITARY MEDICAL SCI +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the bortezomib-based chemotherapy regimen can significantly improve the symptoms of MM patients and delay tumor progression in the short term, almost all MM patients who use bortezomib will develop drug resistance characteristics after a period of medication, which is of great significance. Limits the long-term efficacy of the drug for MM
So far, there is still little research on the mechanism of drug resistance of MM to bortezomib, the mechanism of drug resistance is still unclear, and there is no effective clinical solution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of nucleoside hydrolase 21 in preparation of reagent for detecting and/or treating multiple myeloma
  • Application of nucleoside hydrolase 21 in preparation of reagent for detecting and/or treating multiple myeloma
  • Application of nucleoside hydrolase 21 in preparation of reagent for detecting and/or treating multiple myeloma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Real-Time Reverse Transcript Polymerase Chain Reaction (RT-PCR)

[0041] Total RNA was extracted from RPMI-8226 and SKO-007 cells using Trio Reagent (Invitrogen, Carlsbad, CA, USA). Specific conditions and parameters According to the protocol in the kit instruction manual, 2 μg of total RNA was reversed into cDNA using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (Beijing Quanshijin, China) according to the instruction manual. By using SYBR-Green I as a double-stranded DNA-specific binding dye, real-time PCR was performed in the 7500 detection system, and the system and procedures were carried out according to the Genestar kit instructions. The mRNA of the target gene and the mRNA of the housekeeping gene β-actin were quantified in separate tubes.

[0042] The primers used in RT-PCR are shown in Table 1.

[0043] Table 1 RT-PCR primer sequences

[0044]

[0045] RNA-Seq

[0046] Using the cDNA-PCR Sequencing Kit (SQKPCS109) protocol provided by O...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of nucleoside hydrolase 21 in preparation of a reagent for detecting and / or treating multiple myeloma, and belongs to the technical field of detection and treatment of multiple myeloma. Broad Instrument Cancer Cell Line Encyclopedia data sets are comprehensively analyzed, and research results of the invention show that high expression of NUDT21 in MM cells, including CD138+ cells of an MM patient and MM cell strains RPMI-8226, SKO-007, U266 and XG7, is found. The RPMI-8226 cell and the SKO-007 cell are used for inhibiting the expression level of the NUDT21 after being treated by the bortezomib. Silencing the expression of NUDT21 can significantly inhibit the proliferation of RPMI-8226 cells and SKO-007 cells (including the capacity of cell number amplification and colony formation in a liquid culture medium culture state).

Description

technical field [0001] The invention belongs to the technical field of detection and treatment of multiple myeloma, and in particular relates to the application of nucleoside hydrolase 21 in the preparation of reagents for detection and / or treatment of multiple myeloma. Background technique [0002] Multiple myeloma (Multiple Myeloma, MM) is a hematological malignancy originating from lymphoplasmacytic cell lineage. Its typical feature is abnormal proliferation of plasma cells, resulting in clinical manifestations such as hypercalcemia, renal dysfunction, anemia, and bone disease. The median age at diagnosis of MM was 69 years, and the average overall survival was 6 to 7 years. Chemotherapy is the main treatment for MM; in recent years, with the in-depth exploration of the pathogenesis of MM, researchers have screened out proteasome inhibitors, immunomodulators and other targeted therapeutic drugs from the molecular level. A breakthrough has been made. The clinical use of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886G01N33/574G01N33/573A61K45/00A61K38/05A61P35/00
CPCC12Q1/6886G01N33/57407G01N33/57484G01N33/573A61K45/00A61K38/05A61P35/00C12Q2600/158
Inventor 肖凤君王立生高川成孙慧燕
Owner ACADEMY OF MILITARY MEDICAL SCI