Supercharge Your Innovation With Domain-Expert AI Agents!

A kind of separation breeding method of dendrobium orchid pure color flower

A technology of dendrobium and solid color, which is applied in the field of separation breeding, can solve the problems not related to the method of separation breeding of dendrobium pure color flower, and achieve the effect of large number of pure color flower plants, large particles and high separation rate

Active Publication Date: 2022-05-24
HAINAN UNIV
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neither of the two patents involves the method for the separation and breeding of pure-colored flowers of Dendrobium orchids, but only involves the technical issues of improving the cultivation efficiency of Dendrobium orchids and cultivating new hybrid varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of separation breeding method of dendrobium orchid pure color flower
  • A kind of separation breeding method of dendrobium orchid pure color flower
  • A kind of separation breeding method of dendrobium orchid pure color flower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1 explant selection: Take the lateral bud stem of Dendrobium as the explant, rinse with tap water, soak in diluted detergent for 10 minutes, rinse with tap water, soak with 70% alcohol by volume for 60 s, and then rinse with sterile water for 5 times. Remove leaves and membranous leaf sheaths on a clean bench, and transfer them to 0.1% HgCl containing 1 drop of Tween-80. 2 The solution was sterilized for 8 minutes, rinsed with sterile water 5 times, and dried with sterile gauze or sterile filter paper.

[0026] S2 protocorm induction: inoculate the sterilized side buds on the induction medium with the formula of 1 / 2MS+BA 1mg / L+NAA 0.1mg / L for the first solid-state culture, culture for 45d, culture in light for 14h every day, and culture in dark For 10h, the light culture conditions were light intensity of 1500Lux, temperature of 26°C, and dark culture temperature of 26°C; when the new axillary buds grew to 0.8 cm and there were 2 leaves, the new shoots were cut off, th...

Embodiment 2

[0032] S1 explant selection: Take the lateral bud stem of Dendrobium as the explant, rinse with tap water, soak in diluted detergent for 10 minutes, rinse with tap water, soak with 70% alcohol by volume for 60 s, and then rinse with sterile water for 5 times. Remove leaves and membranous leaf sheaths on a clean bench, and transfer them to 0.1% HgCl containing 1 drop of Tween-80. 2 The solution was sterilized for 8 minutes, rinsed with sterile water 5 times, and dried with sterile gauze or sterile filter paper.

[0033] S2 protocorm induction: inoculate the sterilized side buds on the induction medium with the formula 1 / 2MS+BA 4mg / L+NAA 0.2mg / L for the first solid-state culture, culture for 45 days, and culture in the light for 14 hours every day, and culture in the dark. For 10h, the light culture conditions were light intensity of 1500Lux, temperature of 26°C, and dark culture temperature of 26°C; when the new axillary buds grew to 0.8 cm and there were 2 leaves, the new shoo...

Embodiment 3

[0039] S1 explant selection: Take the lateral bud stem of Dendrobium as the explant, rinse with tap water, soak in diluted detergent for 10 minutes, rinse with tap water, soak with 70% alcohol by volume for 60 s, and then rinse with sterile water for 5 times. Remove leaves and membranous leaf sheaths on a clean bench, and transfer them to 0.1% HgCl containing 1 drop of Tween-80. 2 The solution was sterilized for 8 minutes, rinsed with sterile water 5 times, and dried with sterile gauze or sterile filter paper.

[0040] S2 protocorm induction: inoculate the sterilized side buds on the induction medium with the formula of 1 / 2MS+BA 3mg / L+NAA 0.2mg / L for the first solid-state culture, culture for 45 days, culture in light for 14 hours every day, and culture in dark For 10h, the light culture conditions were light intensity of 1500Lux, temperature of 26°C, and dark culture temperature of 26°C; when the new axillary buds grew to 0.8 cm and there were 2 leaves, the new shoots were cu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for separation and breeding of solid-colored flowers of Dendrobium orchid. The lateral bud stem section of Dendrobium orchid is used as an explant, and the protocorm induction medium is 1 / 2MS+BA 3mg / L+NAA 0.2mg / L, and the protocorm proliferates The culture medium is 1 / 2MS+BA 3mg / L+NAA 0.2mg / L+banana 30g / L+potato 20g / L, combined with the liquid suspension culture and the processing method of cutting off new shoots, the separation and breeding of solid-colored flowers of Dendrobium orchid is carried out, the present invention can Improve protocorm multiplication rate, and effectively separate stable pure-color flowers from dendrobium orchid color chimera, the separation rate reaches 76.09%.

Description

technical field [0001] The invention relates to the technical field of separation and breeding, in particular to a method for separation and breeding of pure color flowers of Dendrobium orchid. Background technique [0002] Dendrobium orchid, orchid family Dendrobium is a perennial herbaceous epiphyte with large racemes, various colors, bright and colorful. Dendrobium orchid has many flower-color chimera varieties, such as red and white flower-color chimeras. Such flower-color chimera varieties are unstable in flower color and pattern during the reproduction process, and it is difficult to keep the same with the mother plant. The solid color flower or monochromatic flower varieties can maintain the stability of the color during the breeding process, and have a high commerciality. [0003] The conventional propagation methods of Dendrobium are ramets, buds and cuttings, but these methods have larger propagules and low reproduction rates. Dendrobium can be rapidly propagated...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 谢光明李秀梅刘进平陈银华
Owner HAINAN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com