Method for separating and purifying trigonelline, chlorogenic acid and caffeine

A trigonelline, separation and purification technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as waste of resources

Active Publication Date: 2021-09-03
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]Coffee planting and processing will produce a lot of waste, including husk, pulp and coffee

Method used

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  • Method for separating and purifying trigonelline, chlorogenic acid and caffeine
  • Method for separating and purifying trigonelline, chlorogenic acid and caffeine
  • Method for separating and purifying trigonelline, chlorogenic acid and caffeine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A. The coffee extract is properly concentrated, and the analysis results are as attached figure 2 As shown, adjust the pH of the extract to 3, and the acidic coffee extract is loaded onto the polyamide adsorption resin column with a flow rate of 4BV / h (BV, resin column bed volume);

[0037] B. Use 5BV of water to elute first, and the analysis results of the eluate are as attached image 3 shown. Carry out elution with 50% ethanol of 5BV again, collect eluate respectively, eluate analysis result is as follows Figure 4 As shown, the chromatographic purity of the chlorogenic acid content in the alcohol eluent obtained was 30.13%.

Embodiment 2

[0039] A. The coffee extract is properly concentrated, adjusted to pH=6, and the acidic coffee extract is loaded onto the polyamide adsorption resin column at a flow rate of 4BV / h;

[0040] B. First eluted with 5BV of water, then eluted with 5BV of 50% ethanol, collected the eluents respectively, and obtained a chromatographic purity of chlorogenic acid content in the ethanol eluents of 31.44%.

Embodiment 3

[0042] A. The coffee extract is properly concentrated, adjusted to pH=4, and the acidic coffee extract is loaded onto the polyamide adsorption resin column at a flow rate of 4BV / h;

[0043] B. First use 5BV of water to elute, then use 5BV of 50% ethanol to elute, collect eluents respectively, and obtain the chromatographic purity of chlorogenic acid content in the ethanol eluent as 31.62%.

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PUM

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Abstract

The invention provides a method for separating and purifying trigonelline, chlorogenic acid and caffeine, belongs to the field of extraction and separation of natural products, and particularly relates to a method for separating and purifying three components, namely trigonelline, caffeine and chlorogenic acid, in a coffee extracting solution. The method comprises the following steps: starting from a coffee extracting solution, adjusting the pH value of the extracting solution to 3-6, loading a sample to polyamide adsorption resin, eluting with water to obtain an alkaloid component, and desorbing with an alcoholic solution to obtain a phenolic acid component mainly comprising chlorogenic acid; concentrating the alkaloid component properly, adjusting the pH value to 7, loading a sample onto non-polar macroporous adsorption resin and eluting with water, obtaining a trigonelline component, and then desorbing the trigonelline component with an alcohol solution to obtain a caffeine component. The process is simple in operation step, short in production cycle, free of toxic and harmful reagents and complex production equipment, and beneficial to expanding the production scale.

Description

technical field [0001] The invention provides a method for separating and purifying trigonelline, chlorogenic acid and caffeine. Specifically, it involves the use of polyamide resin and non-polar macroporous resin as the separation medium, and the combination and optimization of chromatographic methods to separate and purify the main components of trigonelline, chlorogenic acid and caffeine in the coffee extract to obtain higher purity respectively. Trigonelline, Chlorogenic Acid and Caffeine. The invention belongs to the field of natural product extraction and separation. Background technique [0002] Coffee is the most popular non-alcoholic beverage and ranks second in global trade after oil. The composition of coffee beans is complex, and more than 1,500 chemical components have been found in it. The main components include sugars, proteins, lipids, organic phenolic acids, and alkaloids. The biological activity of coffee is mainly attributed to the nerve excitatory eff...

Claims

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Application Information

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IPC IPC(8): C07D473/12C07D213/80C07C69/732C07C67/56
CPCC07D473/12C07D213/80C07C69/732C07C67/56C07C2601/14
Inventor 孙立权张鹏科罗爱芹
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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