[0034] Next, the technical solutions in the embodiments of the present invention will be apparent from the embodiment of the present invention, and it is clearly described, and it is understood that the described embodiments are merely embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, there are all other embodiments obtained without making creative labor without making creative labor premises.
[0035] As described in the background art, the deficiencies in the prior art, in order to solve the above technical problem, the present application proposes a dry, rapid cell smart centrifugal extraction apparatus
[0036] A typical embodiment of the present application, as Figure 1-10 As shown in A Stem cells were centrifuged intelligent rapid extraction apparatus 1 comprises a bottom plate, the top plate 1 is fixedly connected with the base 2, is fixedly connected to a centrifugal motor 3 and the base 2 on the vibration motor 4, the motor output shaft 3 by its centrifugal a centrifugal clutch inner shaft is fixedly connected, the other end of the mandrel extends to the outer side of the centrifugal motor 3 and defines a cross groove 5 is connected, the output shaft of the vibration motor 4 is fixed by its internal coupler has an input shaft, the other end of the input shaft extends to an outer side of the vibration motor 4 and is fixedly connected with a first fixed bearing 9 and the turntable 10, the top plate 1 is fixedly connected with four support posts 6, 6 are on the same side of the two support posts a connecting plate fixedly connected with 7, the top four support columns 6 are fixedly connected with the same reaction tank 11, and the bottom of the reaction tank 11 wherein a top web 7 are fixedly connected with the fixed block and the other two fixed block, the fixed block are three side defines a fixing hole, the fixing hole are fixed to three arcuate sleeved fixed rod 8, one of the two arc-shaped rods 8 are fixed to the fixed connector of the first fixed bearing 9 on the bottom of the reaction tank 11 has a through hole, the through hole 12 rotatably mounted a vibrating rod, both ends of the vibration bar 12 through the through-hole and both extend to the outer sides of the through hole, the vibrating rod 12 is fixed to the top 13 is connected to the vibration plate 13 is located inside the vibration plate 11 the reaction tank.
[0037] Further, in the above-described embodiment, two arcuate end bars 8 are fixed perforations defines a bottom end 12 of the vibrating rod and rotates sequentially through two perforated sleeved within two perforations 12 is fixed on the vibrating rod sleeved there are two swing block 14, a side 14 close to each other are fixedly connected two pivot blocks have the same swing lever 15, the swing lever 15 positions the turntable 10 corresponding to each other, the swing lever 15 slidably sleeved on the elevating ring 16, the outer wall of the lift ring 16 is fixedly connected with a connecting post, the side of the turntable 10 defines elongated hole, the other end of the connecting post extending through the elongated hole and the outer side of the hole to extend through the lift ring 16 is provided with a connecting post, after starting the vibration motor 4, the turntable 10 rotates to drive the swing lever 15 swings, the swing block 14 is then driven so that the vibrating rod 12 and the vibration plate 13 continues to rotate, thereby completing the vibration effects.
[0038] Further, in the above-described embodiment, the top plate 1 is fixedly connected two fixing base 17, the top two are fixed to the fixing base 17 is connected with a collection tube 18, collection tube 18 defines one side of a rectangular collecting aperture, collecting cylinder 18 equipped with the bowl 19, the bowl 19 near one end of the cross groove 5 is connected fixedly connected with a cross connect block 20, the cross connect block 20 located within the cross-connection groove 5 is connected and adapted to the cross groove 5, the bowl 19 defines a plurality of circular holes with a uniform distribution, a plurality of circular holes in positions corresponding to each position of the rectangular collection holes connected by the cross piece 20 is provided with a cross groove 5 of the connector, so that the centrifugal motor 3 starts indirectly driven centrifugation drum 19 rotates at high speed, while facilitating loading and unloading the bowl 19, maintenance operations such as cleaning.
[0039] Further, in the above-described embodiment, one end of the bowl 19 is fixedly connected with injection tube opened and closed by a valve 21, injection tube 21 communicating with the bowl 19, the bowl 19 to the other end defines two positions relative corresponding arcuate groove 22 and the stopper limiting aperture 23, the arcuate grooves 22 and the limit stop in the same relative position of the holes 23 communicating, collecting end of cylinder 18 defines an annular seal groove 24 by the annular seal groove 24 other settings, such that the bowl 19 at high speed can be secured relative to the sealed environment within the collector tube 18.
[0040] Further, in the above-described embodiment, one end of the collection tube 18 is provided with a sealing cover 25, the position of the sealing cover 25 and the position of the annular groove 22 corresponding to the seal, the sealing cap 25 near the side of the annular seal groove 24 is fixedly connected with a second fixed bearing 26, fixed on the inner wall of the second bearing 26 fixed to the rotary shaft 27 is mounted a driven, end of the driven shaft 27 extends to an outer side of the second bearing 26 is fixed and fixedly connected to the seal plate 28, sealing plate 28 by and a second fixed bearing 26, the bowl 19 further ensure a good seal during centrifugation relative, but also improves the safety of the device.
[0041] Further, in the above embodiment, the sealing plate 28 near the two side arcuate stop groove 24 is fixedly connected with two arc-shaped stopper 29, two arc-shaped stopper 29 located in two arcuate limits positioning groove 22 adapted thereto respectively, two sides are arcuate stopper 29 is fixedly connected with a resilient stopper ball 30, four balls 30 are located at the elastic limit stopper hole 23 and two respectively with two holes 23 adapted to limit, by providing the elastic stopper 23 and the stopper hole 30 of the ball, the sealing cap 25 to achieve the limit, to prevent the risk of detachment at high speed.
[0042] Further, in the above-described embodiment, the upper seal cover 25 fixedly connected with a second end connected to the fixed bearing 26 is fixed to an annular ring 31, annular seal 31 is located in an annular sealing groove 24 adapted thereto and collecting cylinder 18 fixedly connected two positions corresponding to the collecting ring on the inner wall 32, a plurality of circular holes 32 are located between the one side two collecting ring 32 are in contact with two collecting rings and the outer wall of the bowl 19, the collection cartridge 18 fixed connection bump on the inner wall, wherein side projections with one side of a collecting ring 32 are fixedly connected with the same electric rail 33, is provided by collecting ring 32, the bowl 19 to avoid centrifuged at high speed when the liquid splashes out from the circular hole and the slot, to improve the usefulness of the device.
[0043] Further, in the above embodiment, the slide is mounted a slider motor 34, the slider 34 and the electric motor 33 is connected to the electric slide rail 33, the slider 34 is fixed to the bottom end of the electric connector connecting pipe 35, the connection pipe 35 equipped with a fourth solenoid valve, the fourth solenoid valve 35 and the connecting pipe adapted to the connection pipe 35 and the arcuate injection tube stopper 21 positions correspond to each other, and adapted, at the top of the base 2 is fixedly connected with table 36, the table top 36 is curved stopper is fixedly connected with a controller 37 and a screw 38, screw 38 is fixed to the output terminal is connected to the hose 39 and injection tube 41, the other end of the hose 39 and connecting pipe 35 is connected, via the discharge hose 39 is provided, placental stem cells facilitate the bowl 19 into the mixture to solid-liquid separation.
[0044] Further, in the above-described embodiment, is fixedly connected to the inner wall of the reaction tank 11 has a box-shaped bacteria removing tube 40, an end of injection tube 41 extends into the housing 11 and is connected to pipe 40 through the filtering type reaction box, the box-shaped bacteria removing side tube 40 defines a plurality of holes uniformly distributed cleaning, washing a plurality of apertures each with an inner wall of the reaction tank 11 is adapted to the side of the injection tube 41 is provided with a first solenoid valve 42, the first solenoid valve 42 and injection tube 41 adapted to the top of the reaction tank 11 is fixedly connected with the water tank 43, an input terminal connected to a screw 38 fixed to the inlet pipe 45, inlet pipe 45 fixedly connected with the feed pipe 44, feed tube 44 through the inlet pipe 38 communicating with the screw 45, the other end of the feed tube 44 extends into the reaction tank 11, the intake side of the tube 44 and the feed pipe 45 are provided with a second solenoid valve 46 and the third solenoid valve 47, the second solenoid valve 46 and the third solenoid valve 47 and feed tube 44 are a water inlet pipe 45 and adapted to, a top plate 48 is provided with a collecting tank, the collecting tank 48 to collect the rectangular hole corresponding to the position, the centrifugal motor 3, the vibration motor 4, screw 38, the first solenoid valve 42, the second solenoid valve 46, the third solenoid valve 47 and the fourth solenoid valve are electrically connected to the controller 37, by setting the collection tank 48, the placental stem cells facilitate the collection solution to thereby facilitate subsequent purification, by providing a plurality of solenoid valves, so that the screw 38 can perform various functions, improve the utilization of the screw 38.
[0045] In use, one end of the sealing cap 25 is aligned collecting cylinder 18, such that the annular seal 31 fitted within an annular seal groove 24, thus making two arcuate stop block 29 extends to the corresponding two arc-shaped limiting slot 22 inner, continue to push the sealing cap 25, so that the two elastic stopper ball 30 is inserted in the restriction hole 23 adapted to seal with the end plate 28 of the bowl 19 are bonded together, thus ensuring the tightness of the bowl 19 relative , both for the stem cells are collected solution also improves the safety of the device.
[0046] Placental fragments simultaneously with the enzyme solution into a reaction tank 11, to start the vibration motor 4, the output terminal of the vibration motor 4 is driven by the rotation shaft through the coupling vibrations, thus making the disk 10 is rotated by the turntable 10 is rotated so that the connector post connecting column 16 so as to drive the lift ring, so that the ring 16 moves down along the vertical direction of the swing rod 15, and driven to swing the swing block 14, so as to drive the vibration bar 14 through the swing block 12 swings the swing, so that the vibrating rod 12 in turn drives the diaphragm 13 swing, the swing speed reciprocating within the reaction tank 11 through the vibration plate 13 such that debris and placental enzyme can be sufficiently mixed, and then quickly so that stem cells isolated from placental debris, to ensure that the extraction effect.
[0047] Moved by the electric controller 37 controls the slider 34, so the connection pipe 35 and the injection tube 21 communicating, injection tube 21 is opened, and the screw 38 and opens the second solenoid valve and a fourth solenoid valve 46, so that the feed tube 44 within the reaction tank 11 mixed placental stem cell mixture drawn into the screw 38, screw 38 through supercharging placental stem cell mixture to be discharged into the discharge tube 39, and the fourth solenoid 35 through a connecting pipe exhaust valve 19 into the bowl, and finally close the screw 38 and the second solenoid valve 46 and the fourth solenoid valve and the injection tube 21, connecting tube 35 and the separation and injection tube 21, for high-speed centrifugation.
[0048] Centrifugal motor 3 starts, output of the motor 3 via the centrifugal clutch so that the mandrel is rotated, and further such that the cross connect block 20 is rotated through the cross connect block 20 such that the bowl 19 is rotated at high speed, centrifugal force causes the placental stem cells mixture of enzyme and thrown centrifugal tube 19, and retained placenta residue fixture 19 within the bowl, such that the stem cells and then was poured into a collecting tank 48 through the rectangular hole collection, and then facilitate subsequent purification, thus realizing the placenta Stem cells was isolated placental residue.
[0049]After the centrifugation is completed, the third solenoid valve 47 and the first solenoid valve 42 are opened such that the water pipe 45 extracts water in the water tank 43 to the screw pump 38, and the boostering of the screw pump 38 will water into the water injection pipe 41.Inside, and inserted into the frame-type sterilifier 40 through the injection pipe 41, it is finally ejected by a plurality of cleaning holes, thereby cleaning a large amount of placenta residue remaining in the reaction box 11, avoiding bacterial infection, affecting the next deviceNormal use.
