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Molecular marker highly related to cockscomb and application of molecular marker
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A molecular marker and highly advanced technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of screening Ningdu yellow chickens, etc., and achieve simple operation, accurate results, and simple and easy methods Effect
Active Publication Date: 2021-09-03
NANCHANG NORMAL UNIV
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However, there is no report on the selection of Ningdu yellow chickens by comb height-related traits.
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Embodiment 1
[0030] Embodiment 1 detects the method for the molecular marker highly correlated with cockscomb
[0031] The PCR method was used to type the corresponding variable sites, and the breeding cocks with different comb heights were selected according to the genotypes of the target sites in the blood of the tested chickens. Specific steps are as follows:
[0033] (1) Blood was collected from 339 16-week-old roosters, and blood DNA was extracted by phenol-chloroform extraction.
[0034] The specific steps for extracting genomic DNA by phenol-chloroform extraction (Osper F, etc., 1998) are as follows:
[0035] ①Put 30 μL of whole blood into a 1.5 mL centrifuge tube, add 470 μL of 1×SET buffer, 12.5 μL of 20% SDS and 6 μL of 10 mg / mL proteinase K, mix well and place in a 55°C water bath overnight;
[0036] ② Take out the sample in a 1.5mL centrifuge tube, add 500μL saturated phenol, shake gently for 20min, and centrifuge at 10,000rpm for 10m...
Embodiment 2
[0065] Example 2 Application of Molecular Markers Related to Comb Height in Selection of Breeding Roosters with Different Comb Heights
[0066] Breeding roosters with different comb heights were selected by the g.1061 genotype of the GH gene locus.
[0075] The obtained PCR amplification product was sent to the biological company for sequencing, and the downstream primer F was used for sequence sequencing. Use the SeqMan module of ...
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Abstract
The invention discloses a molecular marker highly related to a cockscomb and application of the molecular marker, and belongs to the technical field of biological genes. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, an A / G base mutation exists at an SNP site g.1061 of the sequence as shown in SEQ ID NO: 1, and different genotypes of AA, AG and GG appear. The invention also discloses a primer pair for detecting the molecular marker, a kit containing the molecular marker and a method for detecting the molecular marker, and the molecular marker can be used for typing local breeder cocks so as to provide theoretical basis and reference data for breeding of reproductive traits and molecular-assisted breeding work.
Description
technical field [0001] The invention relates to the technical field of biological genes, in particular to a molecular marker highly correlated with cockscomb and its application. Background technique [0002] In the modern poultry industry, many researchers and breeders have realized that breeding roosters play an important role in the level of production, especially affecting the fertilization rate and hatching rate of breeding eggs, which in turn affects the economic benefits of breeding chickens. In addition to qualified henhouse environment and strict feeding management, the selection of reproductive traits is also a very important factor for obtaining high breeding value of breeding roosters. The second characteristics of rooster appearance, such as cockscomb and wattle, are regulated by male hormones, and have different degrees of correlation with each other, which can indirectly reflect the degree of masculinization of roosters, and can be used as breeding indicators ...
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