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Blood collection tube and preparation method and application thereof

A blood collection tube and blood technology, applied in the field of blood collection tubes, can solve problems such as unsatisfactory blood sugar degradation effect, large deviation of test results of blood glucose meter and biochemical analyzer, poor blood sugar degradation effect, etc.

Pending Publication Date: 2021-09-07
北京华益精点生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned existing heparin-based blood collection tubes are not effective in inhibiting blood sugar degradation, sodium fluoride blood collection tubes are not very effective in inhibiting blood sugar degradation, and there is a large deviation in the test results of blood glucose meters and biochemical analyzers

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  • Blood collection tube and preparation method and application thereof
  • Blood collection tube and preparation method and application thereof
  • Blood collection tube and preparation method and application thereof

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preparation example Construction

[0035] In a second aspect, the present invention provides a method for preparing a blood collection tube, the preparation method comprising:

[0036] Add anticoagulant heparin sodium into the vacuum tube, then add glycolysis inhibitor DL-glyceraldehyde and 3-bromopyruvate, and vacuumize according to the preset vacuum capacity to prepare a blood collection tube; or

[0037] Add glycolysis inhibitors DL-glyceraldehyde and 3-bromopyruvate into the heparin sodium blood collection tube, and vacuumize according to the preset vacuum capacity to prepare the blood collection tube.

[0038] According to some embodiments of the present invention, DL-glyceraldehyde with a concentration of 1-2 mg / mL of blood is added, 3-bromopyruvate with a concentration of 0.2-1 mg / mL of blood is added; and / or 1-10 mg of DL-glyceraldehyde is added , add 0.2-5 mg of 3-bromopyruvate.

[0039] According to some embodiments of the present invention, the method of adding DL-glyceraldehyde includes: first addi...

Embodiment 1

[0045] Prepare blood collection tubes:

[0046] (1) Preparation of DL-glyceraldehyde mother liquor: weigh DL-glyceraldehyde and sterile water, prepare DL-glyceraldehyde solution at a concentration of 100 mg / mL, and set aside;

[0047] (2) Prepare 3-bromopyruvate mother liquor: take 3-bromopyruvate and sterile water, prepare 3-bromopyruvate solution at a concentration of 50 mg / mL, and set aside;

[0048] (3) Add DL-glyceraldehyde solution: add the DL-glyceraldehyde mother solution prepared above to the heparin sodium tube according to the ratio of 15 μL of DL-glyceraldehyde mother solution in every milliliter of blood, and add the DL-glyceraldehyde mother solution prepared above according to the capacity of the prepared blood collection tube Add the corresponding dose of DL-glyceraldehyde mother solution to the test tube;

[0049] (4) Add 3-bromopyruvate solution: add the 3-bromopyruvate mother solution prepared above to the sodium heparin tube at a ratio of 10 μL of 3-bromopy...

Embodiment 2

[0051] Example 2 Anti-glycolysis test

[0052] (1) Control group 1: Take common heparin sodium tubes as control group 1, and control group 1 has 3 heparin sodium tube blood collection tubes in total;

[0053] (2) Control group 2: Take sodium fluoride blood collection tubes as control group 2, and control group 2 has 3 sodium fluoride blood collection tubes in total;

[0054] (3) Experimental group 1: Weigh DL-glyceraldehyde and sterile water, prepare DL-glyceraldehyde solution at a concentration of 100 mg / mL, and set aside; Add the above-prepared DL-glyceraldehyde mother solution to the ordinary sodium heparin tube; vacuumize according to the preset vacuum capacity (5mL), and then cover the rubber stopper and safety cap to prepare the blood collection tubes of the experimental group 1, a total of 3;

[0055] (4) Experimental group 2: Weigh 3-bromopyruvate and sterile water, prepare 3-bromopyruvate solution at a concentration of 50 mg / mL, and set aside; according to the ratio ...

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Abstract

The invention provides a blood collection tube and a preparation method and application thereof. The blood collection tube comprises a vacuum tube and a glycolysis inhibitor, the glycolysis inhibitor is placed in the vacuum tube, and the glycolysis inhibitor is DL-glyceraldehyde and 3-bromopyruvic acid. On the basis of the heparin sodium blood collection tube, the DL-glyceraldehyde and the 3-bromopyruvic acid are adopted as glycolysis resisting stabilizers, the glycolysis inhibiting effect of the DL-glyceraldehyde and the 3-bromopyruvic acid and the mutual synergistic effect of the DL-glyceraldehyde and the 3-bromopyruvic acid in the glycolysis inhibiting process are utilized, and therefore the blood sugar is kept not degraded for a long time after the blood is in vitro. Meanwhile, the blood collection tube has little influence on blood glucose testing and biochemical analysis testing, large deviation generated in the testing process can be avoided, the detection result is accurate and reliable, and the problems of misdiagnosis and missed diagnosis caused by glycolysis and testing deviation of a blood glucose meter in the blood collection and treatment process of an existing blood collection tube are solved.

Description

technical field [0001] The invention relates to the technical field of blood collection tubes, in particular to a blood collection tube, its preparation method and application. Background technique [0002] Blood glucose detection is a common clinical test item, and the accuracy of glucose measurement results will directly affect the accuracy of clinical diagnosis. However, since the cells in the isolated blood sample still have glycolysis, and there are many links before the test, the glycolysis will cause the blood glucose concentration to drop during the process of sample operation and sample processing, and the blood glucose concentration has changed before the measurement, which is easy to cause Misdiagnosis, missed diagnosis. [0003] There are many brands of blood preservation tubes on the market, the main components of which are EDTA or heparin and sodium fluoride blood collection tubes. The existing heparin-based blood collection tube can degrade as much as 4% of ...

Claims

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Application Information

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IPC IPC(8): A61B5/154A61B5/15
CPCA61B5/15A61B5/150755A61B5/154
Inventor 王莹
Owner 北京华益精点生物技术有限公司
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