Unlock instant, AI-driven research and patent intelligence for your innovation.

pSOY19-ZM1 vector, preparation method and application thereof

The technology of psoy19-zm1 and 1.psoy19-zm1 is applied in the field of pSOY19-ZM1 vector, which can solve the problems of reducing the content of herbicide-resistant protein in seeds, failing to fully recognize the edible safety of transgenic soybeans, and increasing the accumulation amount, so as to improve the resistance to herbicides. capacity, alleviating safety concerns, and improving yield

Inactive Publication Date: 2021-09-07
ZHEJIANG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The previously obtained herbicide-resistant transgenic soybeans are all constitutively expressed, that is, the resulting transgenic soybean seeds also have EPSPS protein accumulation, and the accumulation is relatively high, which makes the public unable to fully recognize the food safety of genetically modified soybeans. Therefore, the inventors Developed the pSOY19-ZM1 vector, its preparation method and application, and reduced the herbicide-resistant protein content in seeds under the condition of ensuring high-efficiency herbicide resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • pSOY19-ZM1 vector, preparation method and application thereof
  • pSOY19-ZM1 vector, preparation method and application thereof
  • pSOY19-ZM1 vector, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] In this example, the pSOY19-ZM1 vector was first constructed. The pSOY19-ZM1 vector can increase soybean herbicide resistance and reduce the content of herbicide-resistant protein in soybean seeds. The pSOY19-ZM1 vector can be used to increase soybean herbicide resistance and reduce soybean In products with herbicide resistant protein content in seeds.

[0023] In this embodiment, the promoter sequence of 2002bp upstream of the initiation codon of the GmSRS4 gene is first subjected to PCR amplification, and the amplification primers are as follows:

[0024] PGmSRS4-F:

[0025] cccgggtaccgagctcGAATTCAGAAAGTTTCCTATTTGACCGAATGTGG,

[0026] PGmSRS4-R:

[0027] gagactggtgatGAATTCTTGCTTCTCTCTTCTTAGTTTCCAACTTGT; get the amplified PGmSRS4 fragment, spare, see SEQ ID NO.1 for the PGmSRS4 fragment sequence; use EcoRI and NcoI double enzymes to excise the 35S promoter in the plant expression vector pSOY19 vector; then combine the PGmSRS4 fragment with the excised 35S promoter T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of transgenic soybeans, and discloses a pSOY19-ZM1 vector. The pSOY19-ZM1 vector is formed by connecting a green tissue specific promoter PGmSRS4 and a pSOY19 vector with a 35S promoter removed. The invention also discloses a preparation method of the vector and application of the vector. The vector provided by the invention utilizes the soybean green tissue specific promoter to start expression of an anti-herbicide gene G10-EPSPS gene, so that anti-herbicide protein can be expressed in soybean leaves and other green tissues, and can achieve low expression or zero expression in seeds, thus alleviating the concern of the public about the safety of transgenic soybeans, improving the acceptance of the public to transgenic soybeans, at the same time improving the anti-herbicide ability of soybeans, and increasing the soybean yield. The application provided by the invention opens up a more direct and effective way for cultivation of transgenic crops with high-efficiency glyphosate resistance and reduced glyphosate resistance protein in seeds.

Description

technical field [0001] The invention relates to the technical field of transgenic soybeans, in particular to pSOY19-ZM1 vector, its preparation method and application. Background technique [0002] Soybean (GLycine max.Merr.) is an important oil crop and national protein source, which is of great significance in agricultural production. In the process of soybean growth, diseases, insect pests and weeds have seriously affected the yield and quality of soybeans. Compared with foreign soybean production, the competitiveness of soybean production in my country is very weak, and more than 85% depend on imports, which is a "stuck neck" problem. Therefore, there is an urgent need to increase the yield and quality of soybeans in my country and improve the international competitiveness of soybean production by cultivating high-quality resistant strains. [0003] The previously obtained herbicide-resistant transgenic soybeans are all constitutively expressed, that is, the resulting t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/54A01H5/00A01H5/10A01H6/54
CPCC12N9/1092C12N15/8275C12N15/8243C12Y205/01019
Inventor 朱佳美李林王守冬徐恬恬寿惠霞
Owner ZHEJIANG UNIV