Primer and kit for detecting novel goose reovirus and detection method and application thereof

Abstract

The invention discloses a primer and a kit for detecting a novel goose reovirus and a detection method and application thereof. The novel goose reovirus detection primer pair and a probe both target a lambda C gene of the novel goose reovirus. The nucleotide sequences of the novel goose reovirus detection primer pair are as shown in SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide sequence of the probe is as shown in SEQ ID NO.3. The novel goose reovirus detection primer pair and the probe are high in specificity and sensitivity, the lowest detectable limit is 56.6 copies, the detection method is easy to operate and good in repeatability, quantitative analysis can be rapidly and accurately carried out in a high-throughput mode, and application and popularization in clinical practice are facilitated.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a primer, a kit, a detection method and an application for detecting a new type of goose reovirus. Background technique [0002] In recent years, with the large-scale and industrialized development of the waterfowl breeding industry, the breeding density of waterfowl has continued to increase, but the breeding and management level is relatively backward, making it easy for various pathogens to cross-infect among different hosts in large-scale waterfowl farming. Happening. In addition, since some waterfowl are common natural carriers and storage hosts of various viruses, this also exacerbates the complexity and diversification of waterfowl diseases, resulting in new symptoms of old diseases or sudden outbreaks of new infectious diseases . [0003] Since 2020, an infectious disease characterized by punctate white focal necrosis of the liver and spleen has appeared in ...

AI Technical Summary

Problems solved by technology

[0002] In recent years, with the large-scale and industrialized development of the waterfowl breeding industry, the breeding density of waterfowl has continued to increase, but the breeding and management level is relatively backward, making it easy for various pathogens to cross-infect among different hosts in large-scale waterfowl farming. Happening

In addition, since some waterfowl are common natural carriers and storage hosts of various viruses, this also exacerbates the complexity and diversification of waterfowl diseases, resulting in new symptoms of old diseases or sudden outbreaks of new infectious diseases

[0003] Since 2020, an infectious disease characterized by punctate white focal necrosis of the liver and spleen has appeared in some goose breeding and breeding areas. After isolation, identification and sequence determination of the pathogen, it was named Novel Goose Reovirus (NGRV ), but its full genome sequence has not been published

However, there is currently no rapid detection method that can effectively target the new goose reovirus, and the prevalence of the virus in waterfowl populations is not yet clear. Therefore, it is necessary to provide a reliable, effective and rapid method for detecting clinical samples. The method of novel goose reovirus is of great significance to the monitoring, epidemiological investigation and prevention and control of new goose reovirus disease

Images