Application of circular rnahsa_circ_0008399

A circular and gene expression technology, applied in the field of circular RNA technology research, can solve problems such as unsatisfactory overall survival and progression-free survival of bladder cancer patients

Active Publication Date: 2022-05-03
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Progression-free survival and overall survival in bladder cancer patients are unsatisfactory due to cisplatin chemotherapy resistance

Method used

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  • Application of circular rnahsa_circ_0008399
  • Application of circular rnahsa_circ_0008399
  • Application of circular rnahsa_circ_0008399

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] RNA was extracted from 90 pairs of bladder cancer specimens and their corresponding adjacent normal bladder mucosal epithelial specimens, and then reverse-transcribed and then real-time quantitative PCR (same as above) was performed to detect the expression of circ0008399 in bladder cancer specimens relative to adjacent normal bladder mucosa It can be seen that circ0008399 is highly expressed in bladder cancer specimens. figure 2It shows that the expression level of circ0008399 in bladder cancer clinical tissue samples is higher than that in adjacent normal tissues.

Embodiment 2

[0107] The expression levels of circ0008399 detected in 90 cases of bladder cancer samples carried out in Example 2 were divided into two groups (high and low expression). to the date of death or the last follow-up date of the survivor) to obtain the survival curve, it can be seen that the higher the circ0008399, the shorter the survival time. image 3 It shows that the expression level of circ0008399 is negatively correlated with the survival time of patients.

Embodiment 3

[0109] Two kinds of bladder cancer cells EJ and T24T were seeded in a 6-well plate, and the next day when the density was about 60-80%, the small interfering RNA was transfected to knock down the circ0008399 molecule. The transfection reagent used RNAiMAX (Invitrogen, USA), and the steps were as follows According to the instructions (the ratio of serum-free medium: siRNA: transfection reagent is about 100ul: 2ug: 10ul), the cells were harvested after 24h for RNA extraction, reverse transcription, and real-time quantitative PCR to detect the knockdown efficiency of circ0008399. Figure 4 Verify the efficiency of small interfering RNA knockdown circ0008399.

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Abstract

The invention belongs to the field of circular RNA technology research, and specifically discloses the application of circular RNA hsa_circ_0008399. The nucleotide sequence of circular RNA hsa_circ_0008399 is shown in SEQ ID NO.1. Bladder cancer molecular marker, the molecular marker is the circular RNA hsa_circ_0008399. Cisplatin resistance inhibitors include preparations capable of knocking down or knocking out the expression of the circular RNA hsa_circ_0008399 gene. Cisplatin chemosensitivity enhancing drugs in neoplastic diseases, including cisplatin resistance inhibitors. The beneficial effect is: it provides an adjuvant treatment drug for tumor diseases; especially it can better alleviate the drug resistance in cisplatin chemotherapy.

Description

technical field [0001] The invention belongs to the field of circular RNA technology research, and in particular relates to the application of circular RNA hsa_circ_0008399. Background technique [0002] Circular RNA (circRNA) belongs to the family of non-coding RNA transcripts characterized by covalently closed continuous loops. CircRNA is a new type of RNA that is different from linear RNA. Its 3' end and 5' end are linked together. Compared with linear transcripts, it is highly stable, species-conserved, and more resistant to changes in external factors. In 1979, circRNA was first observed in eukaryotic cells by electron microscopy, and it was considered to be a by-product of back-splicing of pre-mRNA with little functional potential. With the development of second-generation RNA sequencing and bioinformatics technology, circRNA has been proved by many well-known researchers at home and abroad to have powerful biological functions, including promoting cancer proliferatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12Q1/6886A61K45/00A61K31/7088A61P35/00
CPCC12N15/113C12Q1/6886A61K45/00A61K31/7088A61P35/00C12N2310/532C12Q2600/106C12Q2600/158C12Q2600/178
Inventor 蒋国松章小平魏文杰张晖肖行远黄超汪良孙加印
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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