A kind of pomelo oil with insecticidal activity against cowpea aphids and its insecticide and application
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A technology of insecticidal activity, grapefruit oil, applied in the direction of insecticide, application, biocide, etc.
Active Publication Date: 2022-05-31
GANNAN NORMAL UNIV
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Literature research shows that there are no related reports about the activity of grapefruit seed oil to kill cowpea aphids and its active chemical components. The present invention has obtained a botanical pesticide that has a significant insecticidal effect on cowpea aphids, and provides green and environmental protection for agricultural control of cowpea aphids. new type of insecticide
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[0036] In the present invention, the volume concentration of the ethanol aqueous solution is preferably 90% to 95%, more preferably 95%. said
[0039] In the present invention, the solvent method for removing the leachate preferably includes concentration under reduced pressure. the present invention to the
[0041] The present invention has no special restrictions on the add-on of water, and it is advisable to fully mix the extract. In this embodiment of the present invention,
[0045] The invention provides the application of the grapefruit oil or the insecticide suitable for the cowpea aphid in the control of the cowpea aphid. Reality
Embodiment 1
Determined by the method of setting conditions. Then 0.1 mL of the C7–C40 n-alkane mixture was dissolved in 0.5 mL of n-pentane, using
GC‑MS equipment and analyzed under the same conditions as above. Finally passed NITS (NIST, 2010 edition, U.S. Department of Commerce, U.S.
(Gaithersburg, Maryland, USA) identified the components of pomelo oil, using the Kovats index formula
I, calculate the actual retention index (RI), and combine with literature comparative analysis.
RI=100Z+100[TR(x)-TR(z)] / [TR(z+1)-TR(z)] Formula 1
Wherein, Z and Z+1 are respectively the number of carbon atoms contained in the normal alkane before and after the outflow of target compound (X), TR
(x) represents the retention time of the target compound x, TR(z) represents the retention time of the n-alkane before the target compound x elutes, TR
(z+1) represents the retention time of n-paraffin after the target compound x elutes).
GC-MS measurement collection of illustrative plates is shown in F...
Embodiment 2
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Abstract
The invention provides grapefruit oil with insecticidal activity against cowpea aphids and its insecticide and application, belonging to the technical field of biological insecticides. The grapefruit oil provided by the present invention contains 21 main active substances, among which D-limonene, (Z, Z)-2,4-decadienal, palmitic acid, and linoleic acid are relatively high in content (the relative content reaches more than 10%) ). The results of the present invention show that the grapefruit oil has a strong insecticidal activity on cowpea aphids through contact and stomach poisoning experiments. When the contact concentration is 2.0 μg / per insect or the stomach toxicity concentration is 20 mg / mL, the effect is 72 hours. After that, the mortality rate of cowpea aphid reached 96.5% and 93.3%, respectively. At the same time, pomelo oil is a plant secondary metabolite extracted from pomelo by-product pomelo seed. It is a natural active substance and has the characteristics of green environmental protection, environmental friendliness and low toxicity. It will not affect human health and is a new type of environmentally friendly insecticide. agent.
Description
A kind of grapefruit oil with cowpea aphid insecticidal activity and its insecticide and application technical field The invention belongs to biological pesticide technical field, be specifically related to a kind of grapefruit oil with cowpea aphid insecticidal activity and Its pesticides and applications. Background technique [0002] Insect pests attack grains such as grains and beans by directly eating plant parts such as buds, seeds, flowers, and plant juices. Widespread damage to food crops. Pests feed on plants, and their waste accumulation is harmful to the plants it eats and to humans, as well as At the same time, pests lead to the decline of crop quality and cause huge economic losses to farmers. According to reports, the annual global agricultural production Nearly 5% to 30% of the amount is infested by pests. Cowpea aphids or black bean aphids are all over the world, especially common in temperate and tropical regions, and it is a dangerous It is a ve...
Claims
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