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An attractant for promoting the secretion of salivary protein of fungal-phagous acarid mites and a method for collecting salivary protein

A technology of saliva protein and food habits, which is applied in the directions of attracting pests, botanical equipment and methods, and material analysis through electromagnetic means, which can solve the problem of low ossification, easy breakage, and increase of high-concentration and high-quality acaria mite saliva protein Difficulty and other issues to achieve the effect of reducing difficulty and increasing secretion

Active Publication Date: 2022-05-27
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the mycophagous mites in the family Acaridae are small in size, the body length of adult mites is 0.1mm-0.9mm, and the mouthparts have degenerated. Insects with sucking mouthparts are collected by double-membrane clamping nutrient solution method; the body wall of acacia mites is thin, the jaw body is less than 0.01 mm, and the degree of ossification is low. It is very easy to break during the process of dissecting the jaw body, and it is difficult to collect enough. A large amount of oral secretions increase the difficulty of obtaining high-concentration and high-quality salivary proteins

Method used

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  • An attractant for promoting the secretion of salivary protein of fungal-phagous acarid mites and a method for collecting salivary protein
  • An attractant for promoting the secretion of salivary protein of fungal-phagous acarid mites and a method for collecting salivary protein
  • An attractant for promoting the secretion of salivary protein of fungal-phagous acarid mites and a method for collecting salivary protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] S1: placing the petri dish base 1 in the second petri dish 5;

[0051] S2: Add clean water to the second petri dish 5 to 1 / 2 of the height of the petri dish base 1;

[0052] S3: place the solvent in the petri dish base 1, the mass percentage of Tween-20 in the solvent is 0.1%, the mass percentage of sucrose is 5%, and the rest is water; the pH of the solvent is 6-7.5;

[0053] S4: Cover the parafilm 2 on the petri dish base 1 placed with the solvent;

[0054] S5: Collect 0.1 g each of Tyrosa carrota mites and Robin root mites adult mites with an 80-mesh filter sieve, transfer them to a 2.0 mL centrifuge tube and starve them for 24 hours, and then wash them with sterilized ultrapure water for 4 times, then use 200~ The 300-mesh gauze 3 is collected, the moisture on the gauze 3 is sucked dry, and it is buckled on the sealing film 2, so that the mycophagous powder mites are located on the sealing film 2;

[0055] S6: Cover the top cover 4 of the petri dish on the gauze 3...

Embodiment 2

[0059] The mass percentage of sucrose in step S3 of this example is 10%, and the rest are the same as in Example 1.

Embodiment 3

[0061] The mass percentage of sucrose in step S3 of this example is 20%, and the rest are the same as in Example 1.

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Abstract

The present invention provides an attractant and a method for collecting salivary protein for promoting the secretion of saliva protein of fungal-feeding acarina acarina. The attractant includes a solvent and an attractant component dissolved in the solvent; the solvent includes an aqueous sucrose solution; The attractant components include 3-octanone and alpha-terpinene. The lure that the present invention provides to promote the secretion of salivary protein of fungiphagous acarid mite, utilizes the volatility of 3-octanone and α-terpinene through the synergistic effect of sucrose aqueous solution and 3-octanone and α-terpinene, to The mycophagous acaroid mites are lured, and then stimulated by 3-octanone, α-terpinene and sucrose aqueous solution to secrete saliva protein, thereby increasing the secretion of saliva protein and reducing the difficulty of obtaining saliva protein .

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular, to an attractant for promoting the secretion of salivary proteins of the mycophagous mites and a method for collecting the salivary proteins. Background technique [0002] Mites belong to Arachnida and Acari. There are currently 5500 genera and 1200 subgenera, and 124 superfamilies and 540 families. my country is a big producer of edible fungi and a big exporter of edible fungi. Mites are an important group that harms edible fungi. There are many types of mites. According to statistics, there are 21 families and 44 species of harmful mites found in my country that are related to the cultivation of edible fungi. , there are as many as 13 species of powder mites, including important pest mites: Tyrophagus putrescentiae, T. longior, Rhizoglyphus robini, Acarus siro )Wait. [0003] Mites can not only feed on mycelium, but also damage fruit bodies. The symptoms of host damage are si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01N35/02A01N27/00A01P19/00G01N1/10G01N27/62
CPCA01N43/16A01N35/02A01N27/00G01N1/10G01N27/62
Inventor 曲绍轩李辉平马林骆昕侯立娟蒋宁林金盛姜建新
Owner JIANGSU ACAD OF AGRI SCI
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