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Method for preparing siamenoside of momordica grosvenori through enzyme-catalyzed semi-synthesis

A technology for mogroside and mogroside, which is applied in the field of enzymatically catalyzed semi-synthetic preparation of mogroside symanside, can solve the problem of no mogroside V being converted into siamanside and the like, and achieves improved overall sweetness, low equipment requirements, and high production efficiency. low cost effect

Inactive Publication Date: 2021-10-08
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] CN201210526355.5 discloses a method for preparing high-purity simonoside I, but the patented method is actually the purification of simonoside, and does not convert mogroside V into simonoside

Method used

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  • Method for preparing siamenoside of momordica grosvenori through enzyme-catalyzed semi-synthesis
  • Method for preparing siamenoside of momordica grosvenori through enzyme-catalyzed semi-synthesis
  • Method for preparing siamenoside of momordica grosvenori through enzyme-catalyzed semi-synthesis

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Experimental program
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Embodiment 1

[0045] (1) Preparation of substrate solution: Substrate (pure mogroside V, 10 g; detected by HPLC external standard method, the content of mogroside V is 98.16%, and the content of simonoside is none) and auxiliary substrate (Shifu element, 0.5 g) into 80 ml of potassium dihydrogen phosphate-sodium hydroxide buffer solution (molar concentration 0.05 mol / L, pH value=6.0), heated to 50° C., and stirred until completely dissolved to obtain a substrate solution;

[0046] (2) The synthesis method of mogroside IIIE: put 0.15g of β-glucosidase into the above substrate solution, keep it warm at 60°C, stir at a speed of 60rpm, and react for 9 hours until the reaction is complete to obtain the reaction solution I; Liquid I was heated to 105°C to inactivate the enzyme for 30 minutes, cooled to 5°C, centrifuged, and filtered to obtain filtrate A containing mogroside IIIE and active β-glucose residues;

[0047] (3) Synthetic method of Mogroside simonoside: add 0.4 g of glucosyltransferase ...

Embodiment 2

[0051] (1) Preparation of substrate solution: Substrate (Mogroside extract, 10g; detected by HPLC external standard method, mogroside V content 50.29%, simonoside content 2.13%) and auxiliary substrate (laminarin, 0.8 g) put into 50ml of potassium dihydrogen phosphate-sodium hydroxide buffer solution (molar concentration 0.05mol / L, pH value=6.5), heat to 50°C, and stir until completely dissolved to obtain the substrate solution;

[0052] (2) The synthesis method of mogroside IIIE: put 0.2g β-glucosidase into the above substrate solution, keep it warm at 57°C, stir at a speed of 90rpm, and react for 10 hours until the reaction is complete to obtain the reaction solution I; Liquid I was heated to 100°C to inactivate the enzyme for 30 minutes, cooled to 5°C, centrifuged, and filtered to obtain filtrate A containing mogroside IIIE and active β-glucose residues;

[0053] (3) Synthetic method of mogroside simonoside: add 0.15 g of glucosyltransferase to filtrate A, incubate at 45° C...

Embodiment 3

[0057] (1) Preparation of substrate solution: Substrate (Mogroside extract, 10g; detected by HPLC external standard method, mogroside V content 65.07%, simonoside content 0.24%) and auxiliary substrate (yeast glucan , 1 g) into 60 ml of potassium dihydrogen phosphate-sodium hydroxide buffer solution (molar concentration 0.05 mol / L, pH value=5.9), heated to 50° C., and stirred until completely dissolved to obtain a substrate solution;

[0058] (2) The synthesis method of mogroside IIIE: put 0.3g β-glucosidase into the above substrate solution, keep it warm at 65°C, stir at a speed of 100rpm, and react for 12 hours until the reaction is complete to obtain the reaction solution I; Liquid I was heated to 105°C to inactivate the enzyme for 30 minutes, cooled to 5°C, centrifuged, and filtered to obtain filtrate A containing mogroside IIIE and active β-glucose residues;

[0059] (3) Synthetic method of mogroside simonoside: add 0.2 g of glucosyltransferase to filtrate A, incubate at ...

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Abstract

The invention relates to a method for preparing siamenoside of momordica grosvenori through enzyme-catalyzed semi-synthesis. The method is characterized by comprising the following steps: adding beta-glucosidase into a substrate solution containing mogroside V and glucan or polysaccharide containing beta-1,6-glucosidic bonds for enzymolysis to obtain a mixture of mogroside IIIE and beta-glucose residues, and then, adding glucosyltransferase for a reaction, thereby obtaining the siamenoside. According to the method, the beta-glucosidase is efficiently utilized; and the mogroside V and the glucan or polysaccharide containing the beta-1,6-glucosidic bonds are subjected to enzymolysis, then, the reaction is carried out under the action of glucosyltransferase to prepare the target product siamenoside, side reactions are few, and the conversion rate is high. According to the method disclosed by the invention, the yield of converting the mogroside V into the siamenoside of momordica grosvenori is 90% or more. The method disclosed by the invention is simple in process, high in operability, low in equipment requirement, mild in reaction condition, low in production cost and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing mogroside simonoside, in particular to an enzyme-catalyzed semi-synthetic method for preparing mogroside simonoside. Background technique [0002] Luo Han Guo is a unique plant in my country. It is mainly produced in Lingui County and Yongfu County of Guilin City. Mogroside is a low-calorie, high-sweet, non-nutritive, non-fermented sweetener, and is the main sweet component in Luo Han Guo. As a sweetener, mogroside is safe and non-toxic, does not affect blood sugar, and does not induce dental caries. According to the national mandatory standard "GB2760 Standards for the Use of Food Additives", mogroside can be used in various foods without limit. Under the background of the current global sugar-free and low-sugar healthy diet, mogroside has become a hot natural sweetener that major food and beverage manufacturers focus on. [0003] Mogrosides include mogroside IIIE, mogroside IV, simonoside, mogroside V...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20
CPCC12P33/20
Inventor 李伟黄华学贺进军黄俊宋谷良江小龙
Owner HUNAN HUACHENG BIOTECH