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Eichhornia crassipes polysaccharide based on antibody targeting, and application thereof in preparation of anti-ALV-J drugs

An ALV-J and antibody-targeted technology, which is applied in the direction of anti-tumor drugs, drug combinations, and medical preparations of non-active ingredients, can solve the problems of few research reports on anti-viral drugs, and achieve enhanced anti-ALV-J activity, The effect of reducing the toxic side effects of organisms

Inactive Publication Date: 2021-10-19
山东德信生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Antigen-antibody binding is an important research field in biomedicine, and anti-tumor drug therapy based on antibody targeting is a hot research direction in biomedicine (Yan Jiayao et al., 2021), but research reports on antiviral drugs based on the affinity of antibodies for viruses are currently Still less (Zhu et al., 2019)

Method used

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  • Eichhornia crassipes polysaccharide based on antibody targeting, and application thereof in preparation of anti-ALV-J drugs
  • Eichhornia crassipes polysaccharide based on antibody targeting, and application thereof in preparation of anti-ALV-J drugs
  • Eichhornia crassipes polysaccharide based on antibody targeting, and application thereof in preparation of anti-ALV-J drugs

Examples

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Embodiment 1

[0042] Embodiment 1: Extraction and purification of water hyacinth polysaccharide

[0043]The freeze-dried water hyacinth stems and leaves are crushed through a 100-mesh sieve, soaked overnight by adding 10 times the weight of water, then leached for 3 hours at 90°C, filtered, and the filtrate is concentrated to a relative density of 1.05-1.15 ( 50 DEG C), centrifuged to remove impurities, to obtain an extract; add ethanol with a volume concentration of 95% to the extract, stir while adding, so that the final concentration of ethanol is 70%, let stand, centrifuge, separate the precipitate, Dry the precipitate at 60°C to prepare ECPs70; add ethanol with a volume concentration of 95% to the supernatant after separation and precipitation, and stir while adding, so that the final concentration of ethanol is 50%, let stand, centrifuge, separate the precipitate, Dry the precipitate at 60°C to prepare ECPs50; mix ECPs70 and ECPs50 evenly, add water to dissolve, adjust the pH value to...

Embodiment 2

[0044] Example 2: Preparation and characterization of polysaccharide complexes of Eichhornia chinensis based on antibody targeting

[0045] 1. Preparation of polysaccharide complex of water hyacinth hyacinth:

[0046] (1) Preparation of Antibody Chitosan Complex (Ab-CS):

[0047] Add 2.2mg of chitosan to 1ml of acetic acid solution with a mass concentration of 1%, stir at room temperature until completely dissolved, adjust the pH value to 5 with 10% NaOH solution, and centrifuge at 4000r / min at low speed for 3min to remove undissolved impurities, and take the supernatant solution into a beaker, add 20 μL of JE9 monoclonal antibody, stir magnetically for 15 minutes, then add catalyst EDC 0.1ml and NHS 0.11ml to activate the carboxyl group of the monoclonal antibody and perform condensation reaction with the amino group of chitosan, and continue stirring for 2 hours. Add 10 times the amount of ethanol to the reaction solution to precipitate the reaction product Ab-CS, centrifug...

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Abstract

The invention discloses eichhornia crassipes polysaccharide based on antibody targeting, and application thereof in preparation of anti-ALV-J drugs, and belongs to the technical field of antiviral drugs. It is found for the first time that the eichhornia crassipes polysaccharide has good inhibitory activity on J subtype avian leukosis viruses and can be developed into the antiviral drugs. In order to further improve the antiviral effect of the eichhornia crassipes polysaccharide, the eichhornia crassipes polysaccharide is subjected to antibody modification, and the eichhornia crassipes polysaccharide compound based on antibody targeting is constructed. The constructed eichhornia crassipes polysaccharide compound can enhance the anti-ALV-J activity of the eichhornia crassipes polysaccharide and reduce the toxic and side effects of organisms.

Description

technical field [0001] The invention relates to the technical field of antiviral drugs, in particular to an antibody-targeted Eichhornia polysaccharide and its application in the preparation of anti-ALV-J drugs. Background technique [0002] Water hyacinth (Eichhornia crassipes) belongs to the genus Hyacinthaceae and the genus Eichhornia crassipes. It is commonly known as water hyacinth. At present, the main use of water hyacinth is to use it to purify sewage. The living body of water hyacinth has a strong removal effect on heavy metals in water, and is used as feed and raw materials for papermaking. [0003] Polysaccharide is one of the active components of Eichhornia chinensis, but there are relatively few research reports on polysaccharides from Eichhornia chinensis. Wang Chengmei extracted polysaccharides from the stems and leaves of water hyacinth by alkaline extraction, separated and purified them, and prepared a new type of superabsorbent resin ("Phoenix phoenix) by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P31/14A61P35/02A61K9/14A61K36/88A61K47/61A61K47/68
CPCA61K31/715A61P31/14A61P35/02A61K36/88A61K47/61A61K47/68A61K9/146
Inventor 刘建柱李克鑫王振峰刘丙艳潘云鑫邢仁增
Owner 山东德信生物科技有限公司