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Enzymes for infusion mashing in adjunct brewing technical field

A technology for assisting brewing and glucoamylase, applied in the directions of glycosylase, beer brewing, enzymes, etc.

Pending Publication Date: 2021-10-22
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So while the use of trim reduces the overall cost of the raw material, it requires additional investment in grain digesters, as well as additional costs for heating and treating the trim to release fermentable sugars

Method used

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  • Enzymes for infusion mashing in adjunct brewing technical field
  • Enzymes for infusion mashing in adjunct brewing technical field
  • Enzymes for infusion mashing in adjunct brewing technical field

Examples

Experimental program
Comparison scheme
Effect test

example

[0117] Example 1 - Enzyme

[0118] GsAA1: an alpha-amylase variant from Geobacillus stearothermophilus, having the amino acid sequence shown in SEQ ID NO:1.

[0119] GsAA2: maltogenic alpha-amylase from Geobacillus stearothermophilus, having the amino acid sequence shown in SEQ ID NO:2.

[0120] TrGA: glucoamylase from Trichoderma reesei, having the amino acid sequence shown in SEQ ID NO:3.

[0121] As an example of an alpha-amylase, used from DuPont (DuPont) 5T (A 5T). As an example of a maltogenic alpha-amylase from DuPont was used MA (D MA). As an example of glucoamylase from DuPont is used TGA (D TGA).

[0122] Example 2 - Protein Assay Method

[0123] Protein Assay by Stain Free Imager Criterion

[0124] Using Gel Doc TM The EZ Imaging System quantifies proteins by SDS-PAGE gels and densitometry. Reagents used in the assay: Concentrated (2x) Laemmli sample buffer (Bio-Rad, Cat. No. 161-0737); 26-well XT4-12% Bis-Tris gel (Bio-Rad , catalog number 345-0125)...

example 3

[0125] Example 3 - Sugar Analysis by HPLC

[0126] All standards: Glucose, maltose, maltotriose and maltotetraose were prepared in double distilled water (ddH20) and filtered through a 0.45 μm syringe filter. A series of each standard was prepared at concentrations ranging from 10 to 100,000 ppm.

[0127] All wort samples containing active enzymes were inactivated by heating the samples to 95°C for 10 min. Wort samples were subsequently prepared in 96-well MTP plates (Corning, NY, USA) and diluted at least 4 times in ddH2O and filtered through a 0.20 μm 96-well plate filter (Corning filter plate , PVDF hydrophilic membrane, New York State, USA). All samples were analyzed in duplicate.

[0128] instrument

[0129] Quantification of sugars: DP1, DP2, DP3, DP4 and DP5+ by UPLC. Sample analysis was carried out in a laboratory equipped with DGP-3600SD dual-gradient analysis pump, WPS-3000TSL constant temperature autosampler, TCC-3000SD constant temperature column oven and RI...

example 4

[0132] Example 4 - Low temperature leach saccharification of corn, rice, sorghum and cassava using enzymes to obtain extracts and fermentable sugars

[0133] The purpose of this example is to demonstrate the presence of two enzymes (maltogenic alpha amylase or glucoamylase and alpha amylase ) benefits (fermentable sugars and extract release).

[0134] The enzymes were tested in a model system of mashing operations for wort production using corn grain (Nordgetreide GmBH Lübec, Germany), rice grain (Cambodia, MEKONG Asian Market), Dagrofa Brabrand), sorghum (white unground sorghum - Diageo, Ireland) and tapioca (Uganda) with a fixed water to grain ratio of 4:1. Rice grains were ground on a Buhler Miag malt mill at a setting of 0.5 mm and sorghum at a setting of 1.6 mm.

[0135] Mashing operations for wort production

[0136] Maize grains (3.0 g), rice grains (ground 3.0 g), sorghum (3.0 g) or tapioca flour (3.0 g) were mixed in wheaton cups (glass containers with lids) and ...

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Abstract

The present invention relates to methods of mashing 100% adjunct grists. More specifically, the instant disclosure provides methods and compositions wherein an alpha-amylase in combination with an maltogenic alpha amylase and / or glucoamylse to make a non-malt wort composed by adjunct raw materials.

Description

technical field [0001] The present invention relates to a method of saccharifying auxiliary cereal starch. More specifically, the present disclosure provides methods and compositions wherein an alpha amylase in combination with a maltogenic alpha amylase and / or a glucoamylase is employed in brewing to provide a malted wort consisting of co-raw materials. Background technique [0002] Brewing usually involves three steps: malting, mashing and fermentation. The main purpose of the malting step is to develop enzymes that have a subsequent effect on starch and protein degradation during the brewing process. Although traditionally beer is only brewed from barley malt, hops and water; malt is an expensive raw material because it requires high-quality grains, water for malting and energy for drying. To reduce raw material costs, the brewing process may include unmalted grains, also known as trimmings, such as maize, rice, tapioca, wheat, barley, rye, oats, quinoa, and sorghum. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L29/30C12C12/00C12C12/02C12P7/06C12C12/04
CPCC12C5/02Y02E50/10C12C7/047C12C5/004C12N9/2411C12N9/2414C12N9/2428C12Y302/01001C12Y302/01003C12Y302/01133
Inventor J·F·克拉米尔T·布拉特
Owner DUPONT NUTRITION BIOSCIENCES APS
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