Three-dimensional forming method and system

Abstract

The invention provides a three-dimensional forming method and system, wherein the method comprises the steps that 1, biological ink in an acoustic resonant cavity is controlled through acoustic waves to form a cell cluster pattern M1; 2, controllable light curing is carried out on a pre-curing position of the cell cluster pattern M1 to obtain a cured and formed structure; 3, the cured and formed structure is transferred; 4, the biological ink in the acoustic resonant cavity is controlled through acoustic waves to form a cell cluster pattern M2, the position of the cured and formed structure in the step 3 in the biological ink is adjusted, and the position is accurately in butt joint with the pre-curing position of the cell cluster pattern M2; 5, controllable light curing is carried out on the pre-curing position of the cell cluster pattern M2 to obtain a cured and formed structure; and the steps 3-5 are circularly executed for a preset number of times to obtain a three-dimensional organization structure. By means of the three-dimensional forming method, three-dimensional printing can be achieved through coupling of the acoustic waves and a photocuring technology, and the accuracy and efficiency of printing of the three-dimensional heterogeneous organization structure are improved.

Description

technical field [0001] The present application belongs to the technical field of tissue engineering, and in particular relates to a three-dimensional shaping method and system. Background technique [0002] In the field of tissue engineering, bionic 3D printing technology is used to three-dimensionally shape materials such as bioinks and cells, and bionic tissue structures can be constructed in vitro for disease models or regenerative medicine research. In the formed three-dimensional tissue structure, the arrangement of cells and the interconnection between cells are critical to the subsequent functional development of the tissue. Conventional bio-3D printing technologies such as extrusion-type bio-3D printing use bio-inks loaded with required cells to extrude gelled fiber filament materials to construct three-dimensional tissue structures. This method cannot control the cells in the gelled filament material, and the number and position distribution of cells in the gel fil...

AI Technical Summary

Problems solved by technology

At present, the method of manipulating, arranging and three-dimensionally shaping cells based on acoustic waves is limited: after the cell patterning is completed, there is no flexible curing control system, and the patterned cells cannot be selectively cured; thus This leads to challenges in the construction of heterogeneous tissue structures by acoustic wave technology, which limits the wide application of this technology in the field of tissue engineering

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