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Immune microsphere chromatography test strip for rapidly detecting heavy metal cadmium and preparation method of immune microsphere chromatography test strip

A technology of immune microspheres and chromatographic test strips, applied in biological testing, measuring devices, analytical materials, etc., can solve problems such as poor specificity and low sensitivity, and achieve simple operation, strong specificity, good practical application value and development potential. Effect

Pending Publication Date: 2021-11-05
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to fill the gap in the technical field of detection of heavy metal cadmium by immune microsphere test strips, and overcome the problems of existing heavy metal cadmium detection technology that require instrument assistance, low sensitivity, and poor specificity.

Method used

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  • Immune microsphere chromatography test strip for rapidly detecting heavy metal cadmium and preparation method of immune microsphere chromatography test strip
  • Immune microsphere chromatography test strip for rapidly detecting heavy metal cadmium and preparation method of immune microsphere chromatography test strip
  • Immune microsphere chromatography test strip for rapidly detecting heavy metal cadmium and preparation method of immune microsphere chromatography test strip

Examples

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Embodiment 1

[0051] A kind of immunomicrosphere chromatography test strip for rapid detection of heavy metal cadmium, such as figure 1 As shown, it includes a bottom plate, and the bottom plate is provided with ①sample pad, ②immune microsphere binding pad, ③color developing area and ④water absorbing area from left to right;

[0052] The test strip width is 4mm.

[0053] ②Immune microsphere binding pad is anti-Cd 2+ -Immune microsphere detection probe formed by ITCBE monoclonal antibody combined with carboxyl microspheres.

[0054] ③The color area includes ⑤T line and ⑥C line; T line is the original coating Cd 2+ -ITCBE-OVA; line C is goat anti-mouse antibody.

[0055] A preparation method of an immunomicrosphere chromatography test strip for rapid detection of heavy metal cadmium, the steps of which are:

[0056] 1) Coated original Cd 2+ -Synthesis of ITCBE-OVA: ITCBE first combined with Cd 2+ Coupling and then coupling with OVA, the molar ratio of ITCBE to OVA is 15:1, to synthesize...

Embodiment 2

[0066] An immune microsphere chromatography test strip for rapid detection of heavy metal cadmium, which includes a bottom plate, which is sequentially provided with ① sample pad, ② immune microsphere binding pad, ③ color developing area and ④ water absorbing area from left to right;

[0067] The test strip width is 6mm.

[0068] ②Immune microsphere binding pad is anti-Cd 2+ -Immune microsphere detection probe formed by ITCBE monoclonal antibody combined with carboxyl microspheres.

[0069] ③The color area includes ⑤T line and ⑥C line; T line is the original coating Cd 2+ -ITCBE-BSA; line C is goat anti-mouse antibody.

[0070] A preparation method of an immunomicrosphere chromatography test strip for rapid detection of heavy metal cadmium, the steps of which are:

[0071] 1) Coated original Cd 2+ -Synthesis of ITCBE-BSA: ITCBE is coupled with BSA first and then with Cd 2+ Coupling, the molar ratio of ITCBE and BSA is 30 / 1, and the efficient heavy metal cadmium complete a...

Embodiment 3

[0079] An immune microsphere chromatography test strip for rapid detection of heavy metal cadmium, which includes a bottom plate, which is sequentially provided with ① sample pad, ② immune microsphere binding pad, ③ color developing area and ④ water absorbing area from left to right;

[0080] The test strip width is 9mm.

[0081] ②Immune microsphere binding pad is anti-Cd 2+-Immune microsphere detection probe formed by ITCBE monoclonal antibody combined with carboxyl microspheres.

[0082] ③The color area includes ⑤T line and ⑥C line; T line is the original coating Cd 2+ -ITCBE-KLH; line C is goat anti-mouse antibody.

[0083] A preparation method of an immunomicrosphere chromatography test strip for rapid detection of heavy metal cadmium, the steps of which are:

[0084] 1) Coated original Cd 2+ -Synthesis of ITCBE-KLH: ITCBE first combined with Cd 2+ Coupling and then coupling with KLH, the molar ratio of ITCBE to KLH is 3000 / 1, to synthesize highly efficient heavy meta...

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Abstract

The invention discloses an immune microsphere chromatography test strip for rapidly detecting heavy metal cadmium and a preparation method of the immune microsphere chromatography test strip. The immune microsphere chromatography test strip comprises a bottom plate, and the bottom plate is sequentially provided with a sample pad, an immune microsphere combination pad, a color development zone and a water absorption zone from left to right; and the combination pad is an immune microsphere detection probe prepared from a red carboxyl microsphere and an anti-Cd < 2 + >-ITCBE-carrier protein (CP) monoclonal antibody. The preparation method of the immunomicrosphere chromatography test strip comprises the following steps of: (1) synthesizing a coating antigen Cd < 2 + >-ITCBE-CP; (2) screening an anti-Cd < 2 + >-ITCBE-CP monoclonal antibody; (3) preparing an immune microsphere probe; and (4) assembling the test strip. The naked eye detection limit of the test strip can reach 0.2 ng / mL, and the test strip has no cross reaction with common heavy metal ions (As < 3 + >, Pb < 2 + >, Cu < 2 + >, Fe < 3 + >, Ni < + > and Cr < 3 + >), and has strong Cd < 2 + > specificity. The test strip is simple to operate, and can realize rapid detection of trace Cd < 2 + > in a sample within 3-5 minutes.

Description

technical field [0001] The invention relates to the technical field of rapid detection of heavy metals, in particular to an immunomicrosphere chromatography test strip for rapid detection of heavy metal cadmium and a preparation method thereof. Background technique [0002] With the rapid development of modern society, heavy metals are widely used in chemical fields such as glass, batteries, paints and coatings. However, the accompanying heavy metal pollution has brought potential harm to human daily life and health. Cadmium is one of the most common highly toxic heavy metals, and is listed as one of the heavy metal pollutants for key protection in my country. Therefore, it is necessary to develop a highly sensitive real-time rapid detection technology to effectively monitor the distribution of heavy metal cadmium in the environment and the pollution control situation. [0003] At present, the disclosed rapid detection methods of heavy metal cadmium include the following: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N33/53G01N33/52
CPCG01N33/577G01N33/54313G01N33/558G01N33/5308G01N33/523
Inventor 王元凤程利增徐乃丰朱巧娟
Owner SHANGHAI NORMAL UNIVERSITY