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Serum complement C3 determination kit and preparation method and application thereof

A kit and reagent technology, applied in the field of biochemical reagent determination, can solve the problems of insufficient raw materials for complement synthesis, inconvenient storage of reagents, expensive instruments and equipment, etc. Effect

Inactive Publication Date: 2021-11-09
中拓生物有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] ③Insufficient raw materials for complement synthesis, such as malnutrition diseases in children;
Double-antibody sandwich immunochemiluminescence assay (ILMA) has high accuracy and sensitivity, but the equipment is expensive, reagents are inconvenient to store, and the price is high; colloidal gold colorimetry is simple to operate and produces fast results, but it can only be used for qualitative research and cannot Quantitative
The latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. However, the prior art Chinese-made kits have the disadvantages of low detection value and poor stability. The present invention aims at The shortcomings of existing complement C3 kits are improved to meet the requirements of clinical testing and chemical analysis

Method used

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  • Serum complement C3 determination kit and preparation method and application thereof
  • Serum complement C3 determination kit and preparation method and application thereof
  • Serum complement C3 determination kit and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] A conventional complement C3 assay kit, which includes reagent R1 and reagent R2.

[0055] Reagent R1 contains the following ingredients:

[0056] Tris-hydrochloric acid (Tris-HCl) buffer solution 50mmol / L;

[0057] NaCl 9g / L;

[0058] Triton x-100 1%;

[0059]proclin300 1 ml / L.

[0060] Reagent R1 has a pH value of 7.5.

[0061] Reagent R2 contains the following ingredients:

[0062] 2-(N-morpholino)ethanesulfonic acid (MES) buffer solution 50mmol / L;

[0063] Mouse anti-human C3 antibody coated latex particles 0.3%;

[0064] Bovine serum albumin (BSA) 5g / L;

[0065] Polyethylene glycol 6000 5g / L;

[0066] Glycine 10g / L;

[0067] Glycerol 10ml / L;

[0068] Polyoxyethylene dioleate 1%;

[0069] proclin300 1ml / L.

[0070] Reagent R2 has a pH of 7.2.

[0071] Wherein said percentage is volume ratio. The particle size of latex particles in reagent R2 is 100nm and 150nm in a ratio of 1:1.

[0072] The preparation method of mouse anti-human C3 antibody coated la...

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Abstract

The invention provides a complement C3 determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 is prepared from the following components: a tris (hydroxymethyl) aminomethane-hydrochloric acid (Tris-HCl) buffer solution, NaCl, a surfactant and a preservative. The reagent R2 is prepared from a 2-(N-morpholino) ethanesulfonic acid (MES) buffer solution, mouse anti-human C3 antibody coated latex particles, polyoxyethylene dioleate, a suspending agent, a stabilizer and a preservative. The invention also provides a preparation method and application of the kit, and the kit is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent assay, in particular to a complement C3 assay kit, and also relates to a preparation method and application of the complement C3 assay kit. Background technique [0002] C3 is the complement component with the highest content in serum, which is mainly synthesized by macrophages and liver. Under the action of C3 convertase, it is cleaved into two fragments, C3a and C3b, and plays an important role in both the classic activation pathway and the alternative activation pathway of complement. effect. [0003] More and more attention has been paid to the dynamic changes of complement in clinical practice. Serum total complement and C3 in patients with gastritis caused by antigen-antibody complexes are significantly decreased. The decrease of serum complement in most patients with systemic lupus erythematosus is related to the deterioration of the disease. Serum C1, C4, C2 and C3 in patients...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/31
CPCG01N33/53G01N21/31
Inventor 解苇生庄学伟刘安娜
Owner 中拓生物有限公司