Tobacco glandular hair development regulator, tobacco upper leaf growth promoting and secretion increasing leaf fertilizer and preparation and application of tobacco upper leaf growth promoting and secretion increasing leaf fertilizer
A regulator and foliar fertilizer technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of regulating and improving the growth and development of glandular hairs on the upper leaves of tobacco, and achieve good positive regulation, glandular hairs The effect of increased density and convenient application
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Embodiment 1
[0025] Example 1: Experimental research on the interaction between methyl jasmonate and gibberellin to induce and promote the growth of tobacco glandular hairs
[0026] The flue-cured tobacco variety tested was common tobacco variety TI 1112, and its stem epidermis was a typical non-secreting protective hair without glandular hairs.
[0027] The seeds of TI1112 were sterilized by 10% sodium hypochlorite and sowed on MS medium to cultivate sterile seedlings. At the four-leaf stage, select aseptic seedlings with consistent growth, optimize and select spraying 100 μM, 200 μM, 500 μM and 1 mM NAA, 6-BA, GA3 and MeJA respectively on the basis of a large number of long-term screening studies, Apply 1 mL of foliar fertilizer solution to each tobacco plant to ensure that all leaves are fully moist; the control group is treated with distilled water; it is sprayed once every two weeks, and sprayed three times in total.
[0028] Using a microscope to observe the surface hairs, it was fo...
Embodiment 2
[0030] Example 2: Experimental research on the interaction between methyl jasmonate and gibberellin to induce the development of the head of tobacco glandular hairs
[0031] The variety of flue-cured tobacco tested was Yunyan 87, a common tobacco variety, and there were three types of glandular hairs: long-stalked glandular hairs, short-stalked glandular hairs and protective hairs (without glandular hair heads, which do not have the ability to secrete).
[0032] Tobacco seeds of Yunyan 87 were sterilized by 10% sodium hypochlorite and sown on MS medium to cultivate aseptic seedlings. At the four-leaf stage, select aseptic seedlings with consistent growth, spray 500 μM MeJA+100 μM GA3, and apply 1 mL of foliar fertilizer solution to each tobacco plant to ensure that all leaves are fully moistened; the control group is treated with sterile water Treatment: Spray once every two weeks, and spray 3 times in total.
[0033] Leaf sugar ester staining observation: Tobacco leaves with...
Embodiment 3
[0035] Example 3: Application of foliar fertilizer to promote secretion of glandular hairs on tobacco upper leaves
[0036] 1. Preparation of glandular hair growth-promoting foliar fertilizer
[0037] Long-term production practice studies have found that the occurrence and development of tobacco glandular hairs not only need appropriate hormone induction, but also need to supplement appropriate nutrients. In order to promote the robustness of glandular hairs, in the research of the present invention, the targeted design can promote the growth of glandular hairs and increase the foliar fertilizer of its secretions, that is, in addition to the combination of methyl jasmonate and gibberellin in appropriate proportions and concentrations. In addition, an appropriate amount of potassium sulfate and calcium nitrate is also added to supplement appropriate nutrients; among them, potassium can promote protein synthesis, cellulose and lignin content, so as to promote the toughness and r...
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