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Paenibacillus strains and methods for their use

A technology of the genus Bacillus and Paenibacillus is applied in the field of bacterial strains and their ability to prevent and control plant diseases and increase plant vigor and crop yield, and can solve the problems of difficult development of active ingredients and high development costs

Pending Publication Date: 2021-12-07
BAYER CROPSCI LP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, active ingredients with new mechanisms of action are difficult and costly to develop

Method used

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  • Paenibacillus strains and methods for their use
  • Paenibacillus strains and methods for their use
  • Paenibacillus strains and methods for their use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0197] Example 1. Antifungal activity of Paenibacillus sp. strains NRRL B-50374, NRRL B-67721, NRRL B-67723 and NRRL B-67724 against powdery mildew of cucumber

[0198] Paenibacillus sp. strain NRRL B-50374, Paenibacillus sp. strain NRRL B-67721, Paenibacillus sp. strain NRRL B-67723 and Paenibacillus sp. The genus strains were grown in soy-based media to produce whole broths. The whole broth is diluted to a concentration of 20% in a mixture of water and organic solvent. The diluted whole broth was applied to young plants, which were subsequently exposed to an inoculum of Cucumber powdery mildew (PODOXA), also known as Cucumber powdery mildew.

[0199] Several days after exposure to the phytopathogen inoculum, each plant was scored for percent pathogen control relative to untreated control plants. Each treatment was evaluated in triplicate and the average percent control was reported.

[0200] All strains had previously been evaluated for antifungal activity in several roun...

Embodiment 2

[0201] Example 2. Antifungal activity of Paenibacillus sp. strains NRRL B-50374, NRRL B-67721, NRRL B-67723 and NRRL B-67724 against Botrytis cinerea

[0202] Paenibacillus sp. strain NRRL B-50374, Paenibacillus sp. strain NRRL B-67721, Paenibacillus sp. strain NRRL B-67723, and Paenibacillus sp. strain NRRL B-67724 in soybean-based media Cultivated to produce a whole broth, which is diluted to concentrations of 20% and 5% in a mixture of water and organic solvent. The diluted whole broth was applied to young plants, which were subsequently exposed to an inoculum of Botrytis cinerea (BOTRCI), also known as Botrytis cinerea.

[0203] Several days after exposure to the inoculum of the phytopathogen, each plant was scored for percent pathogen control relative to untreated control plants. Each treatment was evaluated in triplicate and the average percent control was reported. This analysis was repeated several times and see Table 1 for representative results. Paenibacillus sp. ...

Embodiment 3

[0206] Example 3. Antifungal activity of Paenibacillus sp. strains NRRL B-50374, NRRL B-67721, NRRL B-67723 and NRRL B-67724 against wheat leaf rust

[0207] Paenibacillus sp. strain NRRL B-50374, Paenibacillus sp. strain NRRL B-67721, Paenibacillus sp. strain NRRL B-67723, and Paenibacillus sp. strain NRRL B-67724 in soybean-based media to produce a whole broth, which is diluted to a concentration of 20% and 5% in a mixture of water and an organic solvent. The diluted whole broth was applied to the young plants, which were subsequently exposed to an inoculum of P. tritici (PUCCRT) (also known as wheat rust).

[0208] Several days after exposure to the phytopathogen inoculum, each plant was scored for percent pathogen control relative to untreated control plants. Each treatment was evaluated in triplicate and the average percent control was reported. This analysis was repeated several times and see Table 2 for representative results. Paenibacillus sp. strain NRRL B-50374, P...

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Abstract

The present invention provides a composition comprising a biologically pure culture of a Paenibacillus sp. strain or a cell-free preparation thereof comprising a fusaricidin and a tridecaptin, wherein the fusaricidin is produced with a fusaricidin synthetase gene of fusA encoded by a DNA sequence exhibiting at least 90.5% sequence identity to SEQ ID NO: 3; and the tridecaptin is produced with a nonribosomal peptide synthetase (NRPS) gene of triE encoded by a DNA sequence exhibiting at least 90.0% sequence identity to SEQ ID NO: 7. Also provided are methods of treating an agricultural plant to control a disease and methods of increasing the vigor and / or crop yield of an agricultural plant with the disclosed compositions.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 815,069, filed March 7, 2019, the entire contents of which are incorporated herein by reference in their entirety. technical field [0003] The present invention relates to the field of bacterial strains and their ability to control plant diseases and increase plant vigor and crop yield. In particular, the present invention relates to strains of Paenibacillus sp. producing fusacidin and tridecaptin and their use for maintaining plant health and improving plant growth. [0004] Citing a sequence listing submitted electronically [0005] An official copy of the Sequence Listing is submitted electronically via EFS-Web as a Sequence Listing in ASCII format with the file name "BCS189006WO_ST25.txt", created on February 25, 2020, 185 kilobytes in size, and with the specification Submit together. The sequence listing contained in this ASCII format...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/25
CPCA01N63/25C12N1/205C12R2001/07
Inventor J·博A·格尔茨A·纽曼P·施温特克C·S·泰勒田东兰B·A·塔格
Owner BAYER CROPSCI LP
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