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Preparation method and application of radix ranunculi ternati multi-arm phospholactone substance with breast cancer targeting structure

A targeted structure and cat's claw technology, applied in the biological field, can solve problems such as retention, difficulty in industrialization, and complex components of cat's claw, and achieve low requirements for process equipment, enhanced rapid capture capabilities, and easy industrial application Effect

Inactive Publication Date: 2021-12-17
LUOYANG INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development of cat's claw basically stays in the development of traditional Chinese medicine preparations. There are few studies on the extraction and separation of a single monomer, and it only stays in the laboratory stage, so it is difficult to realize industrialization.
In addition, the composition of cat's claw is very complex, and the cost and expense of separation are relatively high, so generally only stay in basic research and important development

Method used

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  • Preparation method and application of radix ranunculi ternati multi-arm phospholactone substance with breast cancer targeting structure
  • Preparation method and application of radix ranunculi ternati multi-arm phospholactone substance with breast cancer targeting structure
  • Preparation method and application of radix ranunculi ternati multi-arm phospholactone substance with breast cancer targeting structure

Examples

Experimental program
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preparation example Construction

[0029] A method for preparing a cat's claw multi-armed phospholactone substance with a breast cancer targeting structure of the present invention specifically comprises:

[0030] (1) Dry and grind the sterilized cat’s claw, then pass through a 60-mesh sieve, add the sieved cat’s claw powder into water and stir evenly, then add cellulose with a mass fraction of 0.2-0.6% to hydrolyze Enzyme solution, adjust the pH value at about 9.0, enzymolyze for 3 hours under normal temperature stirring, and obtain the enzymolysis solution; wherein, the addition amount of the cellulolytic enzyme solution is based on the amount of each gram of cat's claw powder (referring to the cat's claw after sieving). Powder, the same below) is added in proportion to 4-6ml cellulolytic enzyme solution, preferably 4ml.

[0031] (2) Add pectinase and papain to the enzymolysis solution obtained in step (1) respectively in a certain proportion, adjust the pH value of the mixed system at about 9.0, and enzymoly...

Embodiment 1

[0042] (1) Dry and grind the sterilized cat’s claw, then pass through a 60-mesh sieve, add 5g of the sieved cat’s claw powder into water and stir evenly, then add 20ml of cellulose with a mass fraction of 0.2% for hydrolysis Enzyme solution, adjust the pH value to about 9.0, enzymolyze at room temperature for 3 hours under stirring to obtain enzymolyzate;

[0043] (2) Add 0.01g of pectinase and 0.005g of papain to the enzymolysis solution obtained in step (1) respectively, adjust the pH value of the mixed system to about 9.0, and perform enzymolysis for 2 hours under normal temperature stirring;

[0044] (3) 10 ml mass fraction of 0.1% amylase solution is added to the reaction system obtained in step (2), the pH value of the adjustment system is around 8.0, under stirring at room temperature, filter after enzymolysis for 1 hour, remove the filter residue to obtain certain volume of filtrate;

[0045] (4) Add 1g of folic acid solid, 1g of chitosan sodium alginate solid and 10m...

Embodiment 2

[0048] (1) Dry and grind the sterilized cat’s claw, then pass through a 60-mesh sieve, take 5g of the sieved cat’s claw powder, add it to water and stir evenly, then add 20ml of cellulose with a mass fraction of 0.4% for hydrolysis Enzyme solution, adjust the pH value to about 9.0, enzymolyze at room temperature for 3 hours under stirring to obtain enzymolyzate;

[0049] (2) Add 0.01g of pectinase and 0.005g of papain to the enzymolysis solution obtained in step (1) respectively, adjust the pH value of the mixed system to about 9.0, and perform enzymolysis for 2 hours under normal temperature stirring;

[0050] (3) 10 ml mass fraction of 0.1% amylase solution is added to the reaction system obtained in step (2), the pH value of the adjustment system is around 8.0, under stirring at room temperature, filter after enzymolysis for 1 hour, remove the filter residue to obtain certain volume of filtrate;

[0051] (4) Add 1g of folic acid solid, 1g of chitosan sodium alginate solid ...

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Abstract

The invention relates to a preparation method and application of a radix ranunculi ternati multi-arm phospholactone substance with a breast cancer targeting structure. The preparation method comprises the following steps of drying, grinding and sieving cleaned radix ranunculi ternati, adding into water, carrying out enzymolysis for 3 hours by utilizing cellulose hydrolase under stirring, adding pectinase and papain, carrying out enzymolysis for 2 hours, adding amylase, carrying out enzymolysis for 1 hour, filtering, and adding folic acid, chitosan sodium alginate and a phenylboronic acid solution into the filtrate, stirring and reacting in a water bath kettle at 80-100 DEG C for 10-15 hours, carrying out reduced pressure distillation on the obtained mixed solution for 10 hours, and freeze-drying the obtained liquid to obtain the multi-arm phospholactone substance. The radix ranunculi ternati is used as a raw material, a biological active substance radix ranunculi ternati lactone is obtained by utilizing a biological enzymolysis method, and the radix ranunculi ternati multi-arm phospholactone substance is prepared by reacting the radix ranunculi ternati lactone with folic acid, chitosan sodium alginate and phenylboronic acid, so that a certain theoretical basis is provided for the development of the multi-arm phospholactone substance in the radix ranunculi ternati; and a foundation is laid for industrial development and industrial upgrading in the future.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a cat's claw multi-armed phospholactone substance with a breast cancer targeting structure. Background technique [0002] Cat's claw (Ranunculus ternatus Thunb) belongs to the root part of Ranunculus ternatus Thunb, also known as Sansancao and Ranunculus, and belongs to the herb of Ranunculaceae. It is an unknown weed discovered in Xinyang City, Henan Province in the 1950s. Cat's claw is distributed wildly in Gushi County. It is warm in nature, sweet and pungent in taste, and enters the liver and lung meridian. In recent years, researchers have found that cat's claw has a significant effect on the treatment of malignant tumors. It has anti-tumor, antibacterial, anti-phlegm and stagnation-resolving, immune-regulating, and anti-oxidant effects. It is listed as one of the 63 kinds of Chinese herbal medicines in short supply for national key devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61K47/54A61K47/69A61K47/36A61P35/00
CPCA61K36/71A61K47/545A61K47/542A61K47/6925A61K47/36A61P35/00A61K2236/19
Inventor 舒友菊吕庆刘锦琼薛冬贾晓慧唐静雯
Owner LUOYANG INST OF SCI & TECH
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