Preparation method of household test strip for rapidly detecting dermatophytes
A dermatophyte and test strip technology, applied in the field of biomedicine, can solve the problems of high detection cost, complicated detection methods and difficult detection of dermatophyte, and achieve the effect of reducing misdiagnosis and mistreatment
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[0019] The present invention is described below in conjunction with embodiment.
[0020] A kind of test strip preparation method for household rapid detection of dermatophytes of the present invention comprises the following steps:
[0021] ①Obtain the common and specific antigens of dermatophytes; inoculate the dermatophytes (Trichophyton rubrum) into the culture medium, wash and inactivate the dermatophytes in a sterile environment after growth, then dry the dermatophytes, Grind into powder, extract with alkaline extract after degreasing, and filter with filter paper, check and correct the pH value, then carry out sterile filtration and test, then inoculate in culture medium and cultivate, and finally obtain dermatophyte antigen.
[0022] ② Preparation of dermatophyte monoclonal antibody;
[0023] A standard monoclonal antibody preparation process is attached to the literature search. After the monoclonal antibody is prepared, it undergoes antigen-antibody reactions with co...
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