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Preparation method of household test strip for rapidly detecting dermatophytes

A dermatophyte and test strip technology, applied in the field of biomedicine, can solve the problems of high detection cost, complicated detection methods and difficult detection of dermatophyte, and achieve the effect of reducing misdiagnosis and mistreatment

Pending Publication Date: 2021-12-21
HOSPITAL OF DERMATOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Dermatophytosis is a highly infectious skin disease, which may occur in healthy people and people with immunocompromise, and has a high recurrence rate; due to the clinical manifestations of dermatophytosis and other skin diseases such as dermatitis, psoriasis, The clinical manifestations of eczema and others are sometimes similar, especially for people with immunocompromise, it will be more difficult to detect; the existing detection methods for dermatophytes are more complicated and not suitable for home use, resulting in higher personal detection costs, time-consuming and laborious

Method used

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Experimental program
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Embodiment Construction

[0019] The present invention is described below in conjunction with embodiment.

[0020] A kind of test strip preparation method for household rapid detection of dermatophytes of the present invention comprises the following steps:

[0021] ①Obtain the common and specific antigens of dermatophytes; inoculate the dermatophytes (Trichophyton rubrum) into the culture medium, wash and inactivate the dermatophytes in a sterile environment after growth, then dry the dermatophytes, Grind into powder, extract with alkaline extract after degreasing, and filter with filter paper, check and correct the pH value, then carry out sterile filtration and test, then inoculate in culture medium and cultivate, and finally obtain dermatophyte antigen.

[0022] ② Preparation of dermatophyte monoclonal antibody;

[0023] A standard monoclonal antibody preparation process is attached to the literature search. After the monoclonal antibody is prepared, it undergoes antigen-antibody reactions with co...

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PUM

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Abstract

The invention discloses a preparation method of a household test strip for rapidly detecting dermatophytes. The preparation method is mainly characterized by comprising the following steps: obtaining dermatophytes antigens; preparing a dermatophyte monoclonal antibody; preparing a colloidal gold reagent strip for antigen-antibody reaction; preparing a clinical sample treating fluid; and preparing a complete reagent strip set and an operation instruction. The method adopts the colloidal gold reagent strip to rapidly detect dermatophytes, is suitable for home use or grassroots units, people can check whether they are infected by dermatophytes such as dermatophytosis or onychomycosis by themselves, and then interrogates in internet hospitals or goes to drugstores or purchases antifungal drugs on the internet. The occurrence of misdiagnosis and mistreatment of superficial fungal infection and the economic and time cost of visiting a doctor in a hospital can be effectively reduced.

Description

technical field [0001] The invention relates to a preparation method of a test strip for rapidly detecting dermatophytes at home, and belongs to the technical field of biomedicine. Background technique [0002] Dermatophytosis is a highly infectious skin disease, which may occur in healthy people and people with immunocompromise, and has a high recurrence rate; due to the clinical manifestations of dermatophytosis and other skin diseases such as dermatitis, psoriasis, The clinical manifestations of eczema and others are sometimes similar, especially for people with immunocompromise, it will be more difficult to detect; the existing dermatophyte detection methods are relatively complicated and not suitable for home use, resulting in high cost of personal detection, time-consuming and laborious. Contents of the invention [0003] In view of the above-mentioned technical problems, the purpose of the present invention is to propose a test strip preparation method for rapid det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56961G01N33/54313G01N33/558G01N2333/37
Inventor 刘维达臧晓慧王琼
Owner HOSPITAL OF DERMATOLOGY CHINESE ACAD OF MEDICAL SCI
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