Integrated nucleic acid amplification detection equipment

Abstract

The invention relates to the field of in-vitro diagnosis, in particular to integrated nucleic acid amplification detection equipment. The integrated nucleic acid amplification detection equipment comprises a nucleic acid extraction device which is mounted on a base frame and used for lysing and extracting nucleic acids; a nucleic acid amplification detection micro-fluidic chip which is mounted on the base frame and is used for detecting the negative and positive properties of extracted nucleic acid; an electromagnetic valve I which is mounted on the base frame and is used for controlling the communication state between the nucleic acid extraction device and the nucleic acid amplification detection micro-fluidic chip; an electromagnetic valve II which is mounted on the base frame and is used for controlling the communication state of the nucleic acid amplification detection micro-fluidic chip and the external atmosphere; and a pressure supply device which is mounted on the base frame and is used for providing positive pressure for the nucleic acid amplification detection micro-fluidic chip as a drive. The three steps of nucleic acid extraction, nucleic acid amplification and nucleic acid detection can be completed at a time, the whole nucleic acid detection process is smooth, the equipment has the advantages of being convenient to operate, high in efficiency and the like, and meanwhile the equipment is small in design, convenient to carry, capable of being thrown and disposable.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to an integrated nucleic acid amplification detection device. Background technique [0002] In the prior art, centrifugal microfluidic chips are often used in the field of POCT, which can output detection results conveniently and quickly without professional and technical personnel operating. The centrifugal microfluidic chip can complete sample pretreatment, mixing, accurate volume quantification and detection. [0003] Nucleic acid detection technology is the general term for a series of technologies that directly detect the nucleic acid of pathogens. It is a detection method with high sensitivity, high specificity and large detection throughput. It is widely used in virus detection, congenital genetic disease diagnosis and paternity test etc. [0004] Nucleic acid detection is generally divided into three steps: nucleic acid extraction, nucleic acid amplification and...

AI Technical Summary

Problems solved by technology

At present, commercialized nucleic acid detection reagent products, nucleic acid extraction, nucleic acid amplification and nucleic acid detection are mostly carried out independently. After the previous step is completed, the sample is moved to the subsequent equipment for subsequent steps. It needs to be completed in different regions or even different laboratories, and there are strict requirements on the order of sample transfer. This mode of operation makes the entire nucleic acid detection process not smooth, and generally has the characteristics of cumbersome operation and low efficiency.

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