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Method for quantitatively detecting deposition amount of mesenteric fat in fish and application

A fat deposition and quantitative detection technology, applied in the biological field, can solve problems such as time-consuming and labor-intensive, high operation error rate, error, etc., to achieve the effect of avoiding time-consuming and labor-intensive operation, convenient operation, and complete sampling

Active Publication Date: 2022-01-07
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method is simple and direct, it is prone to rupture of the intestinal tube during actual scraping, which makes the contents of the intestinal tract mixed with it, resulting in large errors in the data
Especially when the number of samples to be processed is large or the size of experimental fish is small, this method has problems such as time-consuming and labor-intensive, a high rate of operational errors, and difficult to standardize operations.

Method used

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  • Method for quantitatively detecting deposition amount of mesenteric fat in fish and application
  • Method for quantitatively detecting deposition amount of mesenteric fat in fish and application
  • Method for quantitatively detecting deposition amount of mesenteric fat in fish and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 provides a kind of detection method of quantitative fish mesenteric fat deposition , including the following steps:

[0054] S1, take out the visceral mass of the fish body, and remove the gallbladder organ;

[0055] S2. Perform rapid dehydration treatment on the viscera sample with absolute ethanol, and drain the sample;

[0056] S3, dyeing the dehydrated sample with Oil Red O dye solution, and draining the sample;

[0057] S4, rinse with absolute ethanol to remove the dye solution remaining on the surface of the sample, and drain the sample;

[0058] S5. Put the sample of S4 into a sealed container filled with absolute ethanol solution, and perform static extraction; centrifuge the obtained extract, and collect the supernatant;

[0059] S6. Measure the absorbance OD value of the S5 supernatant at a wavelength of 510 nm;

[0060] S7. Compare the absorbance OD value of S6 with the absorbance OD value of the oil red O standard curve to obtain the oil re...

Embodiment 2

[0064] Optimization of dehydration time, dyeing time and extraction time in the method of the present invention in embodiment 2

[0065] 1, Optimization of fast spin times

[0066] Since the sample dyeing and extraction process are all in an anhydrous ethanol environment, the sample will be dehydrated by itself. This may interfere with staining and may also affect the accuracy of extract quantification. Therefore, it is necessary to add a rapid sample dehydration step before the staining operation to effectively avoid the adverse effects that may be caused by the substantial dehydration of the sample during the staining or extraction process.

[0067] In order to explore the time parameters of rapid dehydration, the fixed samples of viscera mass were put into absolute ethanol for continuous detection. The test results are shown in Table 1. After soaking in absolute ethanol for 5 minutes, the average dehydration rate of the samples was 35.03%; after soaking for 10 minutes...

Embodiment 3

[0078] Embodiment 3 provides a kind of quantitative analysis method comparing fish mesenteric fat deposition difference , the specific steps are as follows, such as figure 2 Shown:

[0079] 1 Materials and methods

[0080] 1.1 Experimental fish

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Abstract

The invention provides a method for quantitatively detecting the deposition amount of mesenteric fat in fish and application. Based on the characteristic that fat-soluble dye oil red O can specifically color adipose tissues, the method for accurately and conveniently quantifying the deposition of mesenteric fat in fish is developed. A dyeing-extraction technical scheme is adopted, the problems of rough quantification, large errors, inconvenience in operation and the like of a traditional method for directly weighing adipose tissues scraped by a scalpel are solved, and the deposition amount of the mesenteric fat of an individual is obtained through the operation steps of visceral mass sample collection and treatment, sample marking, fixing, dehydration, constant dyeing, rinsing, extraction, quantification and the like. the deposition amount is expressed by the mass of oil red O adsorbed and extracted by a sample, and the method can be used for accurately comparing the difference of mesenteric fat deposition among fish individuals.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and application for quantitatively detecting fish mesenteric fat deposition. Background technique [0002] In recent years, with the popularization and application of high-yield and high-efficiency intensive models, the growth rate of farmed fish has been accelerated, and the marketing cycle has been shortened. However, excessive body fat deposition often occurs, leading to problems such as decline in the health status of the fish itself and food quality. Among them, the mesenteric adipose tissue in the abdominal cavity is the main functional part of most fish to store energy and accumulate fat. Excessive deposition not only causes waste of feed resources, but also directly aggravates obesity and affects the sales of fresh products. [0003] In response to the above-mentioned industrial problems, researchers have tried to carry out research on regulation mechanis...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/30
CPCG01N21/31G01N1/30
Inventor 姜鹏樊佳佳李胜杰杜金星马冬梅雷彩霞
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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