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Magnetic-bead-method nucleic acid extraction kit and extraction method thereof

A nucleic acid extraction reagent and extraction method technology, applied in the field of medical testing, can solve the problems of pollution, cumbersome operation, and low extraction efficiency, and achieve the effect of simple operation, high work efficiency, and easy realization

Pending Publication Date: 2022-01-14
SINGLERA GENOMICS (JIANGSU) LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the multiple steps of nucleic acid extraction by magnetic bead method are generally carried out in different test tubes or kits. Not only does it need to prepare a large number of test tubes, but also needs to continuously transfer samples during the extraction process. On the one hand, it is easy to introduce external pollution, on the other hand The operation is also cumbersome and the extraction efficiency is low

Method used

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  • Magnetic-bead-method nucleic acid extraction kit and extraction method thereof
  • Magnetic-bead-method nucleic acid extraction kit and extraction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: A kind of magnetic bead method nucleic acid extraction kit, such as figure 1 As shown: it includes a box body 1, the box body 1 is provided with a sample feeding chamber 2 and a mixing chamber 3, and a connecting liquid path 4 is provided between the sample feeding chamber 2 and the mixing chamber 3; the mixing chamber 3 is provided with an air hole 31, The sample chamber 2 is provided with a sample hole 21 . The connecting liquid path 4 is respectively connected to the lower ends of the sample adding chamber 2 and the mixing chamber 3 . The lower ends of the sample loading chamber 2 and the mixing chamber 3 are provided with inverted triangular conical surfaces. Both the sample loading chamber 2 and the mixing chamber 3 are connected to the liquid path 4 with inclined bottoms.

[0025] The extraction method using the magnetic bead method nucleic acid extraction kit comprises the following steps:

[0026] ① Add binding solution, magnetic bead solution a...

Embodiment 2

[0033] Embodiment 2: a kind of magnetic bead method nucleic acid extraction kit, such as figure 2 Shown: The difference from Example 1 is that there is a sample injection hole 33 above the mixing chamber for adding the magnetic bead solution, and the sample injection hole must be sealed after adding.

[0034] The specific operation steps are as follows:

[0035] 1. Add binding solution and lysed or converted sample solution from the sample injection hole 21 to the sample injection cavity 2; add magnetic bead solution from the sample injection hole 33 to the mixing cavity, and seal the sample injection hole 33;

[0036] ②The air hole 31 is connected to the air pump, and by controlling the positive and negative pressure of the air pump, the solution in the sample loading chamber 2 is pumped into the mixing chamber 3 and mixed with the magnetic bead solution;

[0037] ③Magnet absorbs the magnetic beads in the solution into the magnetic bead adsorption area 32 of the mixing cham...

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Abstract

The invention discloses a magnetic-bead-method nucleic acid extraction kit and an extraction method thereof. The magnetic-bead-method nucleic acid extraction kit comprises a kit body (1), wherein a sample adding cavity (2) and a mixing cavity (3) are formed in the kit body (1), and a connecting liquid path (4) is arranged between the sample adding cavity (2) and the mixing cavity (3); and the mixing cavity (3) is provided with an air hole (31), and the sample adding cavity (2) is provided with a sample adding hole (21). When the kit is used for extracting nucleic acid from a sample, a plurality of steps only need to be completed in one kit, external pollution is not easy to introduce, and the extraction efficiency can also be greatly increased.

Description

technical field [0001] The invention relates to a magnetic bead method nucleic acid extraction kit and an extraction method, which belong to the technical field of medical detection. Background technique [0002] With the popularization of genetic testing and other technical means, in today's pursuit of high-throughput and automation, the limitations of traditional nucleic acid extraction methods are obvious. At present, since the extraction of nucleic acid by the magnetic bead method can realize automatic extraction and large-scale operations, and the operation is simple and the time is short, the use of the magnetic bead method in the field of medical testing is becoming more and more frequent. [0003] The general steps of nucleic acid extraction by magnetic bead method are lysis or conversion, washing and elution. At present, multiple steps of nucleic acid extraction by magnetic bead method are generally carried out in different test tubes or kits, not only need to prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/26C12M1/00
CPCC12N15/1013
Inventor 刘文佳马龙斌何东华刘蕊宋倩叶盛王欣浩周睿王思格
Owner SINGLERA GENOMICS (JIANGSU) LTD