A kind of Lactobacillus fermentum for preventing and treating hyperuricemia and its composition and application
A technology for Lactobacillus fermentum and hyperuricemia, which is applied in the direction of Lactobacillus, application, drug combination, etc., can solve the problems of unassessed impact and weak targeting of probiotic strains, achieve good safety, and improve intestinal kidney Structural damage, inflammatory response, strong effect on acid production
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Embodiment 1
[0063] A Lactobacillus fermentum GF1800 (Limosilactobacillusfermentum GF1800) for preventing and treating hyperuricemia of the present invention, preservation number: CCTCC NO: M 2021984.
[0064] 1) Isolation and screening of Lactobacillus fermentum GF1800
[0065] Collect microbial screening samples from pickled shredded radish farmhouses in Fuyuan County, Yunnan. Cut the collected samples into pieces and weigh 1g, put them into 9mL sterile normal saline, shake and mix well, then apply 10-fold gradient dilution on MRS solids culture medium at 37°C for 48h. Observe with the naked eye, pick single colonies of different shapes and sizes in the culture medium, and repeatedly streak and purify them; and then preliminarily identify them as lactic acid bacteria by Gram staining and calcium dissolution method, and store the purified strains in 45% glycerol at -80°C Refrigerator for spare.
[0066] a) Morphological observation
[0067] Streak the purified Lactobacillus fermentum G...
Embodiment 2
[0097] A composition of this embodiment, including Lactobacillus fermentum GF1800 live strain and Lactobacillus fermentum GF1800 postbiotic, wherein, Lactobacillus fermentum GF1800 live strain (22.1 billion CFU / g, batch number: 20210523); Lactobacillus fermentum GF1800 postbiotic : After the fermentation of Lactobacillus fermentum GF1800, it is prepared by inactivation, concentration, and spray drying (the number of cells is 50 billion CFU / g, batch number: 20210524); preferably, the live strain of Lactobacillus fermentum GF1800 and the postbiotics of GF1800 are determined by the number of cells contained in them Calculated, its content ratio is 1:1~5.
[0098] 1) Determination of the ability to degrade nucleosides:
[0099] Lactobacillus fermentum GF1800 was inoculated into the MRS liquid medium at an inoculum amount of 5%, activated and cultured at 37° C. for 24 hours, and activated twice continuously. Adjust the OD600 of the activated bacterial liquid to 2.0 with physiologi...
Embodiment 3
[0127] A composition of this embodiment, including Lactobacillus fermentum GF1800 live strain, Lactobacillus fermentum GF1800 live bacteria ≥ 2.0 × 10 10 CFU / g.
[0128] Determination of the nitrite degradation ability of the composition: inoculate the Lactobacillus fermentum GF1800 strain into the MRS liquid medium at an inoculum size of 5%, activate and cultivate at 37°C for 24 hours, and activate twice continuously; Inoculate into MRS liquid medium containing sodium nitrite at an inoculum size of 5%, culture at 37°C in a constant temperature incubator, and take samples every 6 hours to determine the content of sodium nitrite (refer to GB 5009.33-2016 for the determination of sodium nitrite) .
[0129] For the ability of Lactobacillus fermentum GF1800 to degrade nitrite, see Figure 16 . It can be seen that the strain can rapidly degrade nitrite, 30mg / kg nitrite can be degraded to 16.43mg / kg in 6 hours, and the nitrite content can be degraded to less than 1mg / kg in 18 hou...
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