83 results about "Cholestanols" patented technology

The present invention relates to process of extracting cholic acid, deoxycholic acid and bilirubin from bile of pig, ox and sheep. Bile of pig, ox and sheep consists of water in about 97 %, bile acid in about 2.5 % and bilirubin in about 0.4 %; and contains also phospholipid, cholesterol, Na, K, Ca, phosphate, carbonate, small amount of protein, and other components. Fresh bile is treated through cooling, filtering to defat, basic hydrolysis, acidification and organic solvent extracting to obtain bilirubin; the rest solution is further treated through deep saponification, acidification and organic solvent precipitation to obtain the mixture of cholic acid and deoxycholic acid; and the mixture is re-crystallization separated to obtain high purity cholic acid and deoxycholic acid.

The invention discloses a method for brewing super-mature vinegar by adopting red yeast prepared from tartary buckwheat. According to the method, the tartary buckwheat is taken as a main material, and hard liquor yeast or red yeast prepared from the tartary buckwheat is taken as a leavening agent. In order to avoid the damage of a high-temperature process to functional ingredients in the tartary buckwheat and reserve nutrient components therein, the super-mature vinegar brewed by adopting red yeast prepared from tartary buckwheat, which is rich in general flavones and Monacolin K, is obtained by adopting raw grain fermentation and combining with a traditional brewing process method of super-mature vinegar through alcoholic fermentation, acetic fermentation, grain fermentation under pressure, smoking fermentation, vinegar spraying, vinegar decoction and ageing. According to the method disclosed by the invention, raw material selection and process design are reasonable; unique flavors of tartary buckwheat are reserved in the product, and meanwhile, the product is rich in general flavones and Monacolin K. The super-mature vinegar is capable of playing roles in reducing the blood pressure and the blood fat under the synergetic effect and also promoting the effects of the super-mature vinegar to reduce the blood pressure, the blood fat and the cholesterol and improving the immunity while enriching the flavor and the quality of the super-mature vinegar.

The present invention provides a crosslinked amine and amide polymers effective for binding and removing bile salts from the gastrointestinal tract. These bile acid binding polymers or pharmaceutical compositions thereof can be administered to subjects to treat various conditions, including hypercholesteremia, diabetes, pruritis, irritable bowel syndrome-diarrhea (IBS-D), bile acid malabsorption, and the like.

The invention relates to a method for preparing cholesterol reducing eggnog fermenting drink by utilizing lactobacillus and microzyme which produce bile salt hydrolase, belonging to a functional drinkwhich is prepared from the following ingredients: 15% of whole egg pulp, 35% of milk, 7% of saccharose, 0.3% of CMC-Na. and 0.2% of carrageenin, wherein the ratio of the raw materials (the whole eggplug and the milk) to water is 1:1 (W / V). The primary fermentation condition is described as following: streptococcus thermophilus Tx, Lactococcus lactis subsp.lactis KS4 and Lactobacillus casei KL1 are mixed in proportion of 1:1:1, the inoculum size is 3%, and then the mixture is fermented for 8h at the temperature of 43 DEG C. The secondary fermentation condition is described as following: Kluyveromyces marxianus M3 and K1 are compounded in proportion of 1:1 or the inoculum size of the single strain is 1%, and then the composite is fermented for 24-26 h at the temperature of 30 DEG C (mixture strain) or 32-34 DEG C; after ripening is carried out for 1 day at the temperature of 4 DEG C, the reducing rate of raw material cholesterol reaches as high as 89.3%. By utilizing the characteristics of strain high-yielding BSH, exopolysaccharide and the like, the invention has the efficiencies of immunological regulation, cholesterol reducing, tumor prevention and the like, and the problem of egg smell is not only solved, but also the content of raw material cholesterol is efficiently reduced through egg pulp proper sterilization and eggnog secondary fermentation. The eggnog drink preparedby the invention has simple fermentation process, short period and lower cost and is suitable for industrialized production.

The present invention relates to a artificial bear gall and its preparation method. Its composition includes sodium taurodeoxycholate, mixed bile pigments, amino acid, mineral elements, rice bean oil, cholesterol and lecithin. Said invented artificial bear gall can be substituted for natural bear gall.

The invention belongs to the technical field of medicine and discloses a preparation method of sub-microemulsion used for the intravenous injection of polyene yew alcohol phospholipid composite. The sub-microemulsion of the polyene yew alcohol phospholipid composite is basically prepared from polyene yew alcohol, phospholipid, liquid oil, a surfactant, cholesterol, a cholesterol derivative, or / and a similar cholesterol derivative, an addition agent and water for injection according to the effective therapeutic dose. The sub-microemulsion of the polyene yew alcohol phospholipid composite provided by the invention has high medicine carrying quantity and good preparation stability; and the preparation method is simple, convenient and easy and is easy for realizing industrialization.

One aspect is a method for improved preparation of molecular imprinted polymer (MIP) particles, where initial compositions comprising insoluble MIP particles are enriched for those MIP particles that bind a particular target molecule, thus excluding non-binding and weakly binding particles from the final composition. Enrichment is typically accomplished via use of chromatographic methods capable of separating particulate material or by means of agglutination. Another aspect is preparation of improved insoluble MIPs by use of extended micronization of raw MIP particles with a view to expose a large number of binding sites per mass unit of MIP particles. In preferred embodiments the two aspects are combined. The resulting improved MIPs may be used for diagnostic, analytical and therapeutic purposes, notably as orally administered drugs which can bind substances such as cholesterol and bile acids and bile acid salts in the gastrointestinal tract.

The invention provides no-egg low-fat salad sauce, which consists of the following components in percentage by weight: 3.0 to 4.7 percent of octenyl succinic anhydride modified waxy rice starch, 3.0 to 3.7 percent of hydroxypropyl crosslinked glutinous rice starch, 21 to 27 percent of water, 25 to 33 percent of salad oil, 22 to 28 percent of maltodextrin, 9.2 to 9.4 percent of soft sugar, 1.62 to 1.72 percent of salt, 3.2 to 4.2 percent of table vinegar, 0.007 to 0.015 percent of potassium sorbate and 0.01 to 0.04 percent of xanthan gum. According to the no-egg low-fat salad sauce, collaborative emulsification and thickening effects of edible gum and the glutinous rice starch are utilized, so that the use of fresh eggs is avoided, and low-cholesterol and low-fat salad sauce are provided for people; not only be cold-mixed no-egg low-fat salad sauce provided, but also high-temperature-resistant no-egg low-fat salad sauce is provided, so that the application fields of the no-egg low-fat salad sauce can be greatly expanded.

The invention relates to a cholesterol-poloxamer-cholesterol triblock copolymer, a preparation method and application thereof. The triblock copolymer is obtained by taking poloxamer as the basic framework, and connecting cholesterol to both ends by carbonic ester bonds. The preparation method includes: placing poloxamer in a sealed container, adding an alkaline catalyst and an acid binding agent under a nitrogen condition, slowly adding a dichloromethane solution containing cholesteryl chloroformate dropwise, conducting stirring mixing in ice-water bath for 5-30min, then placing the mixture at room temperature to react for 1-72h, after the reaction, at the end of the reaction, reducing the pressure and removing the solvent so as to obtain a crude product; adding a proper amount of distilled water to the crude product, performing extraction with dichloromethane three times, then conducting washing three times with ice water, saturated sodium chloride and 100mM hydrochloric acid in order, and carrying out precipitation by ice ether to obtain a white wax matter; and subjecting the white wax matter to repeated precipitation refining by ice ether, thus obtaining the triblock copolymer. And the triblock copolymer has the advantages of low critical micelle concentration, large drug loading capacity, good dilution stability, simple synthetic process, low cost, and wide application range, etc. (structural formula).

One aspect is a method for improved preparation of molecular imprinted polymer (MIP) particles, where initial compositions comprising insoluble MIP particles are enriched for those MIP particles that bind a particular target molecule, thus excluding non-binding and weakly binding particles from the final composition. Enrichment is typically accomplished via use of chromatographic methods capable of separating particulate material or by means of agglutination. Another aspect is preparation of improved insoluble MIPs by use of extended micronization of raw MIP particles with a view to expose a large number of binding sites per mass unit of MIP particles. In preferred embodiments the two aspects are combined. The resulting improved MIPs may be used for diagnostic, analytical and therapeutic purposes, notably as orally administered drugs which can bind substances such as cholesterol and bile acids and bile acid salts in the gastrointestinal tract.

An external preparation for skin containing an alkylresorcinol and being excellent in temperature stability (especially high-temperature stability) and photostability. More particularly an external preparation for skin comprising (a) from 0.01 to 5% by mass of 4-isobutyl resorcinol or a salt thereof, and (b) from 0.01 to 5% by mass of one or more selected from L-ascorbic acid or a salt thereof and a 3-O-alkyl-L-ascorbic acid or a salt thereof. If desired, (c) one or more compounds selected from (c-1) a sterol skeleton-having compound (e.g., POE phytosterols, POE cholestanols), (c-2) a vitamin E derivative, (c-3) a compound such as polyglycerin fatty acid esters, polyoxybutene-polyglycerin alkyl ethers, sucrose fatty acid esters, and (c-4) a sorbitan skeleton-having compound may be incorporated in the preparation.

The invention relates to facial beautification and health care function beverage made of radix polygonati officinalis and a production method thereof. The facial beautification and health care function beverage is made by adding fruit juice, honey and xylitol into fresh radix polygonati officinalis used as main raw materials and processing through the processes of sorting, cleaning, pulverizing, preboiling, enzymolysis, saccharification, and the like. Starch with low nutritional value in radix polygonati officinalis is converted into glucose which is easy for a human body to adsorb by the sugar of amylase. Phlegm in the radix polygonati officinalis, which can be swollen to form a colloid solution during production preboiling, is used for replacing a thickener in the beverage and preventing the generation of beverage sediments. Traditional Chinese medicine safflower is added into the beverage and can be used as a natural pigment additive of the beverage. The processed beverage not only has the aroma of the radix polygonati officinalis and the fruit juice but also has the effects of nourishing heart, invigorating the blood circulation, reducing cholesterol, removing anguish, quenching thirst, and the like. The invention provides the beverage with facial beautification and health care function, unique flavor, sweet taste and convenient drinking for women with the love of beauty.

A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting a sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising i) nonionic surfactant, polyanion and albumin or ii) a combination of a surfactant selected from the group consisting of polyoxyethylene alkylamine or polyoxyethylene alkenylamine and a surfactant selected from the group consisting of polyoxyethylene polycyclic phenyl ether sulfate and an anionic bile acid derivative, and measuring the formed hydrogen peroxide or a reduced coenzyme; and a reagent used therefor.

The present invention discloses a fat-reducing weight-losing milk shake solid beverage and a preparation method thereof. The beverage is prepared from the following raw materials in parts by weight: 15-20 parts of whole milk powder, 15-18 parts of meal replacement powder, 25-40 parts of soybean protein isolate, 0.5-1.5 parts of minerals, 0.3-0.6 parts of sucralose, 6-8 parts of coconut oil powder,3-5 parts of xylitol, 4-6 parts of medium chain triglyceride, 0.3-0.5 parts of edible essence, 0.2-0.4 parts of food additives and 150-180 parts of water. In the meal replacement milk shake solid beverage, the whole milk powder keeps nutritional ingredients of milk, so that the beverage is suitable for all consumers, especially consumers who need to use meal replacements such as young and middle-aged people; the soybean protein isolate is rich in nutrition and does not contain cholesterol, thereby effectively solving the problem that the meal replacements containing the cholesterol cannot beused for people with high blood fat; and the meal replacement powder prepared by mixing and grinding barley powder, corn powder, chestnut powder, black sesame powder, peanut powder and the like is supplemented, thereby providing people with comprehensive nutrition.

A drug product which can maximize the effect of a cholestanol glycoside useful as an anticancer agent. The present invention provides a liposomal composition containing a glycoside exhibiting antitumor activity, a phospholipid, and a positive-charge-providing substance; the glycoside being composed of GlcNAc-Gal-, GlcNAc-Gal-Glc-, Fuc-Gal-, Gal-Glc-, or Gal- as a sugar moiety, and a hydrophobic compound capable of forming a liposome.

Provided is a sugar-cholestanol compound which can be readily synthesized, and which exhibits a satisfactory antitumor activity.An anticancer agent containing, as an active ingredient, a cholestanol compound represented by the following formula (1):or a cyclodextrin inclusion complexation containing the compound.

The invention discloses a slimming drink including components of 30 parts of apple, 30 parts of grape, 30 parts of strawberry, 30 parts of banana, 10 parts of raw sugar and 300 parts of fruit vinegar. The invention further discloses a preparation method for the slimming drink. The slimming drink tastes good, and further can nourish the skin and promote digestion; the drink is rich in soluble fibers, and can absorb fat and cholesterol effectively; the drink has the advantages of beauty, digestion promotion and weight loss, and is healthy to human bodies; the preparation method is simple in preparation process, the raw materials are easy to obtain, the cost is low, and the preparation method is adaptable to industrial production.

The invention relates to a preparation method of an active microbial preparation of lactobacillus casei KL1 of produced bile salt hydrolase, which is applicable to the production of microecological preparations such as feeding functional active microbial preparations in the poultry farming industry. According to the invention, the active microbial preparation is prepared by utilizing lactobacillus casei KL1 strains selected from produced bile salt hydrolase in Tibetan kefir, carrying out activation, enlarged cultivation, high-density fermentation and centrifugal concentration, adding a freeze-drying protective agent, freezing in advance, carrying out freeze drying and the like. According to the invention, the fermentation conditions are optimized by using a fully automatic fermentation tank, and the optimal combination formula of the freeze-drying protective additive is determined by taking potato pulp as a freeze-drying protective matrix, so that the active microbial number of the KL1 strains can be more than 1010 CFU / g, and the survival rate can be more than 85%. The active microbial preparation prepared by using the method disclosed by the invention is low in price and high in active microbial content, can be used as a poultry feed additive, and has the characteristics of rapid chicken growth speed, less feed consumption, and few illnesses and the like. The active microbial preparation not only has an effect of reducing cholesterol in egg, but also has a function of adjusting the balancing of intestinal floras of chickens, and has a better prevention and treatment effect on pullorum diseases.

The invention provides medicinal sodium cholesteryl sulfate and a preparation method thereof. By controlling the addition proportion of cholesterol, sulfamic acid and sodium hydroxide, the content ofsodium ions in the product is within the range of 4.5%-4.9%, it is guaranteed that the final reaction product is basically pure sodium cholesteryl sulfate and hardly contains cholesterol sulfate, anda qualified raw material is provided for safely and effectively applying sodium cholesteryl sulfate.

The invention provides an entecavir compound nano-preparation and a preparation method thereof, and belongs to the technical field of entecavir medicinal preparations. The preparation comprises the following components in parts by weight: 3-6 parts of entecavir, 3-11 parts of phospholipid, 1-3 parts of cholesterol, 0.2-2 parts of cholic acid, 0.2-2 parts of bile salt, 0.5-4 parts of vitamin E and 1-6 parts of mycose, wherein the cholic acid and the bile salt are added in equal weight part. The preparation method comprises the following steps: preparing an organic solution from phospholipid, cholesterol, cholic acid, bile salt and vitamin E; and preparing an entecavir solution from entecavir with phosphate buffer; mixing the two solutions, stirring, volatizing, granulating and lyophilizing the mixture to prepare dry powder of the entecavir compound nano-preparation. The entecavir nano-compound micell in the entecavir compound nano-preparation is small in size and narrow in grain diameter distribution, and has high bioavailability; the preparation method of the compound nano-preparation is easy to operate, and high in production efficiency.

A sugar-cholestanol compound which can be readily synthesized and which exhibits a satisfactory antitumor activity. An anticancer agent containing, as an active ingredient, a cholestanol compound represented by the following formula (1):or a cyclodextrin inclusion complexation containing the compound. A method for treating cancer using this compound.

The invention discloses a phytosterol composition for cosmetics. The phytosterol composition for cosmetics consists of the following raw materials in parts by weight: 1 part of behenyl alcohol, 1-5 parts of cetyl alcohol, 1-5 parts of phytosterol and 0.1-10 parts of phytosteryl ester. According to the phytosterol composition, cholesterol and cholesteryl ester in skin lipid are simulated, phytosterol and phytosteryl ester are used as active ingredients, cetyl alcohol and behenyl alcohol are carefully selected to improve the solubility and melting point of a system, and the phytosterol composition is obtained through a better weight part ratio. The problem that in the prior art, phytosterol and phytosteryl ester are added at the same time and are poor in intersolubility is solved, and meanwhile the obtained composition is good in oil solubility, low in melting point, high in operability in a cosmetic formula and beneficial to industrial application of phytosterol. The obtained additive is applied to cosmetics, the skin care characteristic of phytosterol can be effectively exerted, and the skin friendliness of the product can be improved.

The disclosed chitosan sauce food comprises sauce type substance and chitosan concentration liquid, wherein the chitosan concentration liquid is prepared from 500g distilled water, 4g chitosan and 0.85g acid in normal temperature and sealed container after complete dissolving. The invention also discloses the process for preparing the food.

The invention provides a novel industrial method for preparing vitamin D3 by taking stigmasterol as a raw material. The method comprises the following steps: sequentially carrying out hydroxyl acetylation, side chain oxidation, side chain isopentane reduction and hydrogenation on stigmasterol to obtain cholesterol acetate, and then sequentially carrying out oxidation, hydrazone formation, hydrazone removal, hydrolysis, illumination and the like to obtain the vitamin D3. The invention provides a novel method for preparing vitamin D3 from stigmasterol, and the method has the advantages of mild reaction conditions and high yield, and is suitable for industrial production.

The invention discloses functional protein having bile acid adsorbing capacity and a preparation method of the functional protein. The preparation method comprises the following steps of respectively preparing soybean protein hydrolysate and whey protein hydrolysate through pepsin; and then preparing a mixture of the soybean protein hydrolysate and the whey protein hydrolysate into a solution of which a substrate mass percentage is 25-45%, and performing modification under the condition of pH being 4-7 and the temperature being 22-52 DEG C for 1-6h with the pepsin so as to obtain the functional protein having the bile acid adsorbing capacity. The invention provides the preparation method of the novel protein. Besides, the function characteristics of soybean protein and whey protein for adsorbing bile acid are improved, and health-care efficacies are provided for reducing cholesterol and preventing cardiovascular diseases.

The invention discloses deep-fried fish tofu and a preparation method thereof. The deep-fried fish tofu is prepared from the following raw materials in part by weight: 10-20 parts of fish meat, 50-60 parts of water, 10-20 parts of soy isolate protein, 10-20 parts of salad oil, 1-5 parts of salt, 2-5 parts of sugar, 5-8 parts of seasoning and 5-8 parts of flavoring oil; the deep-fried fish tofu has the beneficial effects that the color and the luster of the skin of tofu is golden yellow, the tofu contains a great amount of water, and the deep-fried fish tofu has tendered and crispy mouthfeel; the fish meat is matched with the soy isolate protein, the nutrition of the soybeans is kept, and inorganic salt, vitamin A, vitamin D, vitamin B1, vitamin B2, vitamin B12, magnesium element, folic acid and other low-fat mineral substances are added, so that the deep-fried fish tofu has rich nutrition; salad oil at 130 DEG C is adopted for frying, the frying temperature is low, and the nutritional ingredients such as protein can be fully kept; and compared with the other salad oils, the adopted salad oil does not contain cancerogenic substances (aflatoxin and cholesterol), thereby facilitating the human health.

The invention discloses pork pickled with soy sauce and a preparation method thereof. The method comprises the following steps: (1) cleaning fresh pork, coating with fish sauce, then putting the pork into saline water, and pickling for 4 to 5 days at a room temperature to obtain pickled pork; (2) taking traditional Chinese medicine materials, decocting and gathering medicinal liquid; taking and pulverizing condiments, and mixing the pulverized condiments with the medicinal liquid; (3) putting the pickled pork into a product obtained in the step (2), carrying out ultrasonic treatment for 50 to 80 minutes, and then continuously soaking for 2 to 3 days; (4) taking the pickled pork subjected to the ultrasonic treatment, putting the pickled pork into a mixed solution of L-alanine and ascorbic acid to be soaked for 5 to 8 hours; then soaking into black tea water for 3 to 4 hours; taking out the pickled pork; sequentially smearing mel and vegetable oil on the surface of the pickled pork, and baking for 5 to 10 minutes to obtain the pork pickled with the soy sauce. The pork pickled with the soy sauce prepared by the method disclosed by the invention almost contains no nitrite, and cholesterol of the pork pickled with the soy sauce disclosed by the invention is also much lower than that of the same kind of the pork pickled with the soy sauce, thereby reducing the eating risk.