Application of tectoridin in preparation of medicine for treating autoimmune diseases and serious inflammatory diseases
An irisin and drug technology, applied in the field of medicine, can solve the problem of no direct connection between T cells and mitochondrial uncoupling proteins, and achieve the effect of broadening the selection field
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[0026] 1. Preparation of irisin
[0027] It can be obtained by yeast expression and purification, and existing methods can be used, such as the document Irisin Stimulates Browning of White Adipocytes through Mitogen-Activated Protein Kinase p38 MAPKinase and ERK MAP Kinase Signaling.Diabetes.Diabetes 2014Feb; 63(2):514-25 records that Iris The preparation process of the element.
[0028] 2. Screening and culturing of primary human CD3+ T cells
[0029] (1) Human peripheral blood mononuclear cells (PBMC) were separated by Ficoll density gradient centrifugation, washed 3 times with phosphate buffer (pH7.2), and the cells were reselected;
[0030] (2) Add CD3+ T cell positive sorting magnetic beads to the reselected cells in step (1) according to the ratio in the instructions, mix well and incubate at 4°C for half an hour;
[0031] (3) Place the sorting column on the sorting rack, and pass 1ml of phosphate buffer solution through the column to equilibrate twice;
[0032] (4) A...
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