Chromatography column packing medium recovery
A chromatographic column and column tube technology, which is applied in the field of chromatographic column packing medium recovery method, can solve the problem of inability to recover separation medium and the like, and achieve the effect of being easy to use
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example 1
[0100] Example 1 - Pressure Test Assembly with Side Ports
[0101] Figure 14A -D shows details of the components involved in the pressure-tested design of the chromatography column with holes and side ports. An assembly of the bottom flow distributor 24B inserted 9.0 mm into the tube (measured from the bottom of the tube to the face of the flow distributor) was constructed and ports were installed in the tube sidewall. The bottom flow distributor is contained in the pipe where there will be 3 cement wedges glued to the pipe wall.
[0102] Insert the top flow distributor 24A into the tube and pressurize the assembly to 90 PSI. This test showed that the chromatography column with the side ports was integral and could contain a pressure higher than twice the factor of safety (FOS) of the maximum rated working pressure of the component, which is 45 PSI.
example 2
[0103] Example 2 - Numerical simulation of the hydrodynamic properties of a 45 cm chromatography column with and without ports
[0104] Computational Fluid Dynamics (CFD) is used to simulate fluid flow through a chromatography column with or without ports on the side walls of the chromatography column. A 45 cm column is considered the worst case for this application because it has a smaller internal diameter and the port will account for a larger percentage of the internal diameter than a 60 cm column. This means that the port may affect the fluid path for a larger portion of the 45 cm column.
[0105]The model takes into account the axisymmetric volume of the chromatography column, splits it in half and smashes it into 4,516,230 elements. Fluid enters through the top screen and exits through the bottom screen. The bottom bed was treated as a porous medium with a volume of 32.82 L (bed height 20 cm), and the fluid velocity through the chromatography column was tested at a st...
example 3
[0108] Example 3 - Flow characteristics of chromatography columns with and without ports
[0109] The port design and placement within the column tubing yielded no change in flow characteristics and chromatographic performance attributes compared to the unmodified chromatography column. This is shown by standard packed column efficiency testing methods (ie, determining theoretical plates and asymmetry). A size exclusion resolution test using molecular weight markers was also performed to further confirm performance. with and without filler ports Columns are filled with 6FF resin, the dimension of each chromatography column is 45 cm inner diameter × 20 cm height. Efficiency tests were performed by injecting 1% bed volume of a 2% acetone solution. The mobile phase consisted of 0.1 M NaCl and was run at a linear velocity of 100 cm / hr.
[0110] Table 1 Column Efficiency
[0111] Plates / m@100cm / hour Asymmetric @100cm / hour standard design 2636±200 1.1±0....
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