Piezoelectric capillary liquid drop preparation device and use method thereof

Abstract

The invention provides a piezoelectric capillary liquid drop preparation device and a use method thereof, the piezoelectric capillary liquid drop preparation device comprises a capillary needle tube, a liquid storage container and a piezoelectric ceramic piece, the capillary needle tube is arranged at the front end of the liquid storage container, and the piezoelectric ceramic piece is fixed on the side wall of the liquid storage container. The device is simple in structure, the piezoelectric ceramic piece is pasted on the liquid storage container, after excitation voltage is applied, the capillary needle tube at the end of the liquid storage container vibrates, liquid drops at the needle tip rotate due to vibration, when the liquid drops reach a certain size, the liquid drops are thrown out by centrifugal force, and by controlling the magnitude of the applied excitation signal, the liquid drops can generate different rotating speeds, and liquid drops with different sizes are generated.

Description

technical field [0001] The invention relates to the technical field of droplet preparation, in particular to a piezoelectric capillary droplet preparation device and a method for using the same. Background technique [0002] Droplet preparation technology is a rapidly developing field, widely used in biochemistry, medicine, materials and other fields, such as digital droplet PCR, drug screening, single-cell RNA sequencing, cell 3D culture technology, nanomaterial chemistry. The droplet preparation technology has experienced traditional methods such as emulsification, microfluidic microfluidic technology, and new off-chip methods in recent years. Traditional droplet preparation methods such as emulsification and layer-by-layer assembly are still widely used due to their simple process and high efficiency. , Accurate and controllable demand. [0003] Microfluidic technology has small size, fast response, and precise control of droplet size, but it has the disadvantages of hi...

AI Technical Summary

Problems solved by technology

However, to avoid high polydispersity in droplet size, the throughput of droplet generation is relatively low (<2 mol / min)

[0005] To sum up, microfluidic technology requires the preparation of microchannel chips, and the process cost is extremely high. Other off-chip technologies also need to use motors and other equipment, and the structure is relatively complicated, while simple structure such as needle-based system droplet preparation technology cannot be prepared. Droplets of different sizes need to replace injection needles with different inner diameters

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