Fab fragment of human anti-human CD23 antibody, pharmaceutical composition and application of Fab fragment

An antibody and fragment technology, applied in the direction of drug combination, application, antibody, etc., can solve the problem of unclear regulation mechanism of B lymphocyte IgE production

An antibody and fragment technology, applied in the direction of drug combination, application, antibody, etc., can solve the problem of unclear regulation mechanism of B lymphocyte IgE production

CN114014931AInactive Publication Date: 2022-02-08南京英瀚斯生物科技有限公司
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  • Fab fragment of human anti-human CD23 antibody, pharmaceutical composition and application of Fab fragment

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Embodiment 1

[0048] This example provides a screening method for Fab fragments of human anti-human CD23 antibodies. It includes:

[0049] (1) Construction of antibody screening library. Specifically, 270 healthy people were selected, and 5ml of peripheral anticoagulated blood was taken each, and lymphocytes were separated with Ficoll-Paque, and after mixing, they were used for the construction of an antibody library, and total RNA was extracted. The total RNA was reverse-transcribed into cDNA with Oligo(dT)16 using the reverse transcription kit of the complete gene, and the immunoglobulin γ, κ, λ chain genes were carried out with the upstream and downstream primers of the conserved sequence of the human CD23 light and heavy chain variable region. PCR amplification.

[0050] After the PCR product is purified, the κ chain and λ chain products are double digested. The digested κ chain and λ chain products were connected to the human immunoglobulin Fab expression vector pFab-His2, and then ...

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Abstract

The invention discloses a Fab fragment of a human anti-human CD23 antibody, a pharmaceutical composition and application of the Fab fragment. The Fab fragment comprises a light chain V region and a heavy chain V region, the amino acid sequence of the light chain V region is shown as SEQ ID NO.1, and the amino acid sequence of the heavy chain V region is shown as SEQ ID NO.2. The Fab fragment can specifically recognize human CD23, has relatively high affinity, and can be used for regulating and controlling the activity of the CD23, so that the adhesion of mediated cells is controlled, the release of IgE and histamine is regulated, and the apoptosis of B cells can be avoided and the growth of bone marrow cells can be regulated by regulating and controlling the activity of the CD23. The Fab fragment of the human anti-human CD23 antibody can be used for preparing medicines for treating lymph-related diseases.

Description

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Claims

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Application Information

Patent Timeline
08 Feb 2022
Publication
CN114014931A