Fab fragment of human anti-human CD23 antibody, pharmaceutical composition and application of Fab fragment

An antibody and fragment technology, applied in the direction of drug combination, application, antibody, etc., can solve the problem of unclear regulation mechanism of B lymphocyte IgE production

Inactive Publication Date: 2022-02-08
南京英瀚斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology provides an improved version of this type of molecule that recognizes both humans (CD) and their own receptors on immune system cell surfaces called TCRs or gamma chains. It allows these proteins to bind with specific parts of certain types of white blood cells like helper leukocytes which help them fight against other harmful substances such as bacteria. These modifications make usable drugs targeted at cancer treatment without causing side effect(s).

Problems solved by technology

This patented technical problem addressed in this patents relates to identifying an immune response from certain parts of bacteria called helper T cells found within blood vessels during inflammations caused by viruses such as influenza virus, rhinovirus, cytomeganoma, chickenpox, measles, tuberculosis, malaria, hepatitis A, streptocystis pneumoniae, salmonella enterica serum, polioviridae, hemorrhagglutins, tumors associated with autoimmunity, neurology disorders like Parkinson' s disease, multiple systematic myopathies, Alzheimer’s diseases, autism spectrum syndrome, fragile X syndromes, spongiform motility group Dementiae, glomeruloneuronarcomas, Graft versus host reaction, transplant recipients, cancer patients, infants, sickness due to helminth attacks, and other conditions involving stimulated responses against self components.

Method used

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  • Fab fragment of human anti-human CD23 antibody, pharmaceutical composition and application of Fab fragment

Examples

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Effect test

Embodiment 1

[0048] This example provides a screening method for Fab fragments of human anti-human CD23 antibodies. It includes:

[0049] (1) Construction of antibody screening library. Specifically, 270 healthy people were selected, and 5ml of peripheral anticoagulated blood was taken each, and lymphocytes were separated with Ficoll-Paque, and after mixing, they were used for the construction of an antibody library, and total RNA was extracted. The total RNA was reverse-transcribed into cDNA with Oligo(dT)16 using the reverse transcription kit of the complete gene, and the immunoglobulin γ, κ, λ chain genes were carried out with the upstream and downstream primers of the conserved sequence of the human CD23 light and heavy chain variable region. PCR amplification.

[0050] After the PCR product is purified, the κ chain and λ chain products are double digested. The digested κ chain and λ chain products were connected to the human immunoglobulin Fab expression vector pFab-His2, and then ...

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Abstract

The invention discloses a Fab fragment of a human anti-human CD23 antibody, a pharmaceutical composition and application of the Fab fragment. The Fab fragment comprises a light chain V region and a heavy chain V region, the amino acid sequence of the light chain V region is shown as SEQ ID NO.1, and the amino acid sequence of the heavy chain V region is shown as SEQ ID NO.2. The Fab fragment can specifically recognize human CD23, has relatively high affinity, and can be used for regulating and controlling the activity of the CD23, so that the adhesion of mediated cells is controlled, the release of IgE and histamine is regulated, and the apoptosis of B cells can be avoided and the growth of bone marrow cells can be regulated by regulating and controlling the activity of the CD23. The Fab fragment of the human anti-human CD23 antibody can be used for preparing medicines for treating lymph-related diseases.

Description

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Claims

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Application Information

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Owner 南京英瀚斯生物科技有限公司
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