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Marker for predicting non-syndromic cleft lip and palate and application thereof

A technology for cleft lip and palate and syndrome, which is applied in the fields of biotechnology and medicine, can solve problems such as complex facial structure of fetuses, and achieve the effects of easy promotion, improved detection efficiency, and simple operation process.

Active Publication Date: 2022-02-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex structure of the fetal face, the accuracy of this method is affected by factors such as the skill of the sonographer, the position of the fetus, the physical fitness of the parturient, the amount of amniotic fluid, and the type of cleft lip and palate.
However, there is no report on the use of serum screening technology for prenatal diagnosis of NSCL / P.

Method used

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  • Marker for predicting non-syndromic cleft lip and palate and application thereof
  • Marker for predicting non-syndromic cleft lip and palate and application thereof
  • Marker for predicting non-syndromic cleft lip and palate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Detection of NSCL / P by Screening Maternal Serum Protein

[0039] Detection of NSCL / P by screening maternal serum proteins APOA, HPT and CRP ( figure 1 ).

[0040] For pregnant women aged 22-34, who have been healthy in the past, are not complicated with gestational hypertension, diabetes and other systemic diseases, and have no history of genetic diseases that affect development. P, early morning fasting venous blood can be collected from the mother during the second trimester of pregnancy (16-28 weeks);

[0041] Take 2mL of venous blood and place it in a blood collection tube without additives, let it stand at room temperature for 2 hours, centrifuge at 2000g for 20min, and carefully absorb the supernatant, which is the serum;

[0042] The expression levels of proteins such as APOA, HPT and CRP in serum were detected by ELISA method, and the results were as follows: figure 2 shown. The suggested dilution concentrations of serum are 2000 times, 300000 ti...

Embodiment 2

[0047] Example 2: Screening maternal serum protein to determine whether fetal cleft lip is accompanied by cleft palate

[0048] pass figure 1 The method shown is to screen maternal serum protein APOA to determine whether fetal cleft lip is accompanied by cleft palate: For pregnant women aged 22-34, they have been healthy in the past, have no systemic diseases such as pregnancy-induced hypertension and diabetes, and have no developmental disorders. Genetic medical history, ultrasound examination shows singleton pregnancy with obvious cleft lip, and it is not sure whether the fetus is accompanied by cleft palate, you can collect early morning fasting venous blood from the mother in the second trimester of pregnancy (16-28 weeks); collect serum and ELISA to detect APOA protein level, the process is the same as the implementation example 1;

[0049] If the maternal serum APOA>56.95μg / mL, the probability of the fetus suffering from NSCLP is 84.375%, and the specificity is 68% (Tab...

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Abstract

The invention relates to a marker for predicting non-syndromic cleft lip and palate and application thereof. The marker comprises one or more of apolipoprotein A, haptoglobin and C-reactive protein. In application, detection reagents of one or more of apolipoprotein A, haptoglobin and C-reactive protein are prepared into a product for predicting fetal non-syndromic cleft lip and palate, and the product can be a chip or a kit. The risk of non-syndromic cleft lip and palate can be accurately predicted by detecting the expression level of the marker in maternal serum in the middle stage of pregnancy through the products, and particularly, whether fetal cleft lip is accompanied by cleft lip or not can be determined by screening apolipoprotein A in maternal serum protein.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a marker for predicting non-syndromic cleft lip and palate and its application. Background technique [0002] Cleft lip and palate is a common congenital oral and maxillofacial deformity, accounting for two-thirds of all facial deformities, and its global incidence is about 1:700. In addition to facial deformities, children with cleft lip and palate are usually accompanied by speech, hearing, eating, jaw development and psychological disorders, which bring heavy mental pressure and economic burden to the family and society. According to the anatomical site, cleft lip and palate can be divided into cleft lip only (CLO), cleft lip with palate (CLP) and cleft palate only (CPO). CLO and CLP are usually combined into one group, that is, cleft lip with or without the palate (CL / P). In addition, according to whether it is accompanied by other general or craniofacial congenit...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/775G01N2333/4737G01N2800/385
Inventor 孟柳燕王欣欢边专
Owner WUHAN UNIV
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