Culture method of saccharomycetes seed solution

A culturing method and technology for yeast, applied in fungi, adding compounds to stimulate growth, etc., can solve the problem of low number of yeast live bacteria, improve yield, increase the number of live bacteria and strain vigor, and promote yeast effect of bacterial growth

Pending Publication Date: 2022-02-11
广东希普生物科技股份有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

but has a low number of viable yeast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, a kind of cultivation method of yeast seed liquid

[0023] A method for cultivating yeast seed liquid, comprising the following steps:

[0024] S1. Cultivation of primary seed liquid: measure 50 mL of prepared primary liquid culture in a 250 mL Erlenmeyer flask, sterilize at 120°C for 10 minutes, inoculate a loop of yeast strains preserved in a test tube after cooling, and inoculate at 25 ℃, 200r / min shaker culture for 20h, the first-grade seed liquid is obtained;

[0025] S2. Preparation of secondary fermentation broth: Measure 1L of the prepared secondary liquid culture in a 5L Erlenmeyer flask, sterilize at 120°C for 10 minutes, and add primary seed solution with 3% volume of secondary liquid medium after cooling , to obtain the secondary fermentation liquid;

[0026] S3. Cultivation of the secondary fermentation broth: place the secondary fermentation broth obtained in step S2 in a magnetic stirrer for stirring at a stirring speed of 500r / min, cultiv...

Embodiment 2

[0030] Embodiment 2, a kind of cultivation method of yeast seed liquid

[0031] A method for cultivating yeast seed liquid, comprising the following steps:

[0032] S1. Cultivation of primary seed liquid: measure 80 mL of prepared primary liquid culture in a 250 mL Erlenmeyer flask, sterilize at 121°C for 20 minutes, inoculate yeast strains preserved in a ring of test tubes after cooling, and inoculate at 30 ℃, 200r / min shaker culture for 20h, the first-grade seed liquid is obtained;

[0033] S2. Preparation of secondary fermentation broth: Measure 2L of the prepared secondary liquid culture based on a 5L Erlenmeyer flask, sterilize at 121°C for 20min, and add 5% primary seed liquid of the volume of the secondary liquid medium after cooling , to obtain the secondary fermentation liquid;

[0034] S3. Cultivation of the secondary fermentation broth: place the secondary fermentation broth obtained in step S2 in a magnetic stirrer for stirring at a stirring speed of 800r / min, cu...

Embodiment 3

[0038] Embodiment 3, a kind of cultivation method of yeast seed liquid

[0039] A method for cultivating yeast seed liquid, comprising the following steps:

[0040] S1. Cultivation of primary seed liquid: Measure 100 mL of prepared primary liquid culture in a 250 mL Erlenmeyer flask, sterilize at 125°C for 30 minutes, inoculate yeast strains preserved in a loop of test tube after cooling, and inoculate at 28 ℃, 200r / min shaker culture for 24h, that is, the first-grade seed liquid;

[0041] S2. Preparation of secondary fermentation broth: Measure 3L of the prepared secondary liquid culture based on a 5L Erlenmeyer flask, sterilize at 125°C for 30min, add 10% primary seed liquid of the volume of the secondary liquid medium after cooling , to obtain the secondary fermentation liquid;

[0042] S3. Cultivation of the secondary fermentation broth: place the secondary fermentation broth obtained in step S2 in a magnetic stirrer for stirring at a stirring speed of 1000r / min, cultiva...

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Abstract

The invention belongs to the technical field of fermentation engineering, and particularly relates to a culture method of a saccharomycetes seed solution. An existing yeast seed liquid fermentation process is optimized, the yield of the final yeast seed liquid is greatly increased through the culture steps of culturing the first-stage seed liquid, preparing the second-stage fermentation liquid, culturing the second-stage fermentation liquid and supplementing materials in the culture process, and meanwhile by adding the accelerant in a specific proportion, through interaction of all the components, the viable count and the strain activity in the yeast culture process can be effectively improved, and the culture efficiency of the yeast seed solution is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for cultivating yeast seed liquid. Background technique [0002] Yeast is a single-celled eukaryotic microorganism that reproduces asexually by budding and can also form ascospores for sexual reproduction. It has the characteristics of large size, high protein content (45% to 55%), easy separation and culture, many metabolites and comprehensive utilization In modern industry, yeast is a kind of microorganism most closely related to people's daily life. It is widely used in the fields of food, medicine and feed industry. Among them, the application of yeast in feed industry is particularly important. With the development of science and technology People are paying more and more attention to the application and development of yeast in feed fermentation. However, the yeast needs to be activated with seed liquid before fermentation, and the amoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/16
CPCC12N1/38C12N1/16
Inventor 王俊青朱帆余忠丽
Owner 广东希普生物科技股份有限公司
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