Culture solution, and method for improving quality of maternal diabetic ova

A culture medium and diabetes technology, applied in the field of reproductive biology, can solve the problems of reduced egg fertilization rate, reduced embryonic development potential, high disease risk, etc., and achieve the effect of improving fertility problems, no toxic side effects, and improving egg quality.

Pending Publication Date: 2022-02-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The fertilization rate of low-quality eggs is reduced, and the developmental potential of emb...

Method used

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  • Culture solution, and method for improving quality of maternal diabetic ova
  • Culture solution, and method for improving quality of maternal diabetic ova
  • Culture solution, and method for improving quality of maternal diabetic ova

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 culture fluid

[0054] The culture medium includes the following components: tea polyphenols (50 μM), heat-inactivated fetal bovine serum (volume fraction 5%), sodium pyruvate (0.25 mM), 100× penicillin and streptomycin (volume fraction 0.75%), FSH (0.5 U / ml) and α-MEM medium.

Embodiment 2

[0055] Embodiment 2 culture fluid

[0056] Except that the concentration of tea polyphenols is 25 μM, other formulations and concentrations are the same as in Example 1.

Embodiment 3

[0057] Embodiment 3 The method for improving maternal diabetes egg quality

[0058] (1) Constructing a maternal diabetic animal model, and isolating the cumulus-oocyte complex from the maternal diabetic animal model: The maternal diabetic animal model was established by intraperitoneal injection of 220 mg / kg body weight of STZ. First select the body weight of 7-8 week old mice, and determine the amount of STZ. Dissolve STZ with citric acid-sodium citrate buffer on ice in the dark, and inject no more than 100 microliters per mouse. After 4 days, blood was taken from the tip of the tail, and the blood glucose concentration was measured with a blood glucose meter. If it was greater than or equal to 17.0 mM / L, the model was considered successful. 10 days after successful model construction, intraperitoneal injection of PMSG 8IU, ovaries were collected 46-48 hours later, the follicles were punctured with a 1ml syringe needle under a dissecting microscope, and COCs were collected. ...

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PUM

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Abstract

The invention discloses a culture solution, and a method for improving the quality of maternal diabetic ova, and relates to the technical field of reproductive biology. The culture solution is used for in-vitro culture of maternal diabetic cumulus-oocyte complexes, the culture solution comprises tea polyphenols, in-vitro culture is performed in the culture solution containing the tea polyphenols, and the quality of the ova is effectively improved. The tea polyphenol used in the culture is from a tea leaf extract, belongs to a natural active substance, has no toxic or side effect on human bodies, animals and the like, is good in safety, and is easy to obtain and low in cost. The culture solution provided by the invention can improve the quality of maternal diabetic ova and improve the fertility problem of maternal diabetic patients.

Description

technical field [0001] The invention relates to the technical field of reproductive biology, in particular to a culture solution and a method for improving the quality of maternal diabetic eggs. Background technique [0002] With the development of society and economy, the problem of human infertility is becoming more and more serious. At present, about 15% of infertile couples in the world, up to about 30% in some countries and regions, of which 40-50% of infertility is caused by maternal factors. Decreased egg quality is one of the most important causes of infertility caused by maternal factors. Many internal and external factors in the process of egg maturation can lead to low egg quality, such as abnormal spindle, uneven separation of chromosomes, and decreased mitochondrial function. The fertilization rate of low-quality eggs is reduced, the developmental potential of embryos is reduced, and it is also easy to lead to a higher risk of disease in newborns and even adul...

Claims

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Application Information

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IPC IPC(8): C12N5/075C12N5/071
CPCC12N5/0609C12N5/0682C12N2501/999C12N2500/30C12N2501/31
Inventor 葛照嘉赵磊卢俊
Owner QINGDAO AGRI UNIV
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