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Recombinant human Sema4C protein, expression vector, host cell and application

A technology for expressing vectors and recombinant proteins, applied in animal/human proteins, vectors, applications, etc., can solve the problem of easy generation of inclusion body soluble proteins, and achieve the effect of rapid transfection cycle and short transfection cycle.

Active Publication Date: 2022-02-15
WUHAN AIBO TAIKE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the recombinant expression of human Seam4C protein is generally based on prokaryotic hosts, but prokaryotic expression is prone to produce inclusion bodies and soluble proteins without biological activity

Method used

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  • Recombinant human Sema4C protein, expression vector, host cell and application
  • Recombinant human Sema4C protein, expression vector, host cell and application
  • Recombinant human Sema4C protein, expression vector, host cell and application

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Embodiment Construction

[0018] In order to make the purpose, technical solutions and advantages of the present application, the present application will be described in further detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are intended to explain the present application and is not intended to limit the present application.

[0019] The human SEMA4C protein is a new IV transmembrane Semaphorin family that is isolated from human abbreviation, and its encoding gene is composed of 13 exons, and the chromosome is positioned in 2q11.2, which encodes 833 amino acids, molecular weights. It was 92.6kD for a single transmembrane protein. The human SEMA4C encoding gene is full of SEQ ID NO.1, the inventors have found that selection of several domains in the region 21-633aa region, perform in vitro recombination expression, and can also produce some of the function of human SEMA4C protein, also Anti-tumor research provides help. The specific im...

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Abstract

The invention discloses a recombinant human Sema4C protein, an expression vector, a host cell and application. A plurality of structural domains in a 21-633aa region of a human Sema4C gene are selected, recombinant plasmids of the structural domains are constructed, eukaryotic cells HEK 293F cells are transfected, in-vitro recombinant expression is carried out, and target proteins of related structural domains are obtained. The method has the effects of being short in transfection period and capable of rapidly obtaining the target protein, and can be applied to exploration in the research and development stage and large-scale high-throughput protein screening.

Description

Technical field [0001] The present application relates to the field of SEMA4C gene, in particular to recombinant human SEMA4C proteins, expression vectors, host cells, and applications. Background technique [0002] The Semaphorins family is a secretion or transmembrane protein, which is the factor that exhibits axial surgery to function in the nervous system. Most of the protein uses its receptor PLEXINS and Neuropilins family to play a corresponding biological effect, including: development Adjust cell movement, angiogenesis, immunomodulation, tumor formation and stem cell characteristics. Some Semaphorin family members are involved in the separation of epithelial cell barriers, which is related to Tumor Epithelial-Mesenchymaltransition (EMT), for example, SEMA3e accumulates SNAIL in the nucleus to induce EMT by activating PI3K / ERK / MAPK. For example, SEMA4C plays an important role in regulating the directional growth of nerve axons and the development of tubular tube, and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/85C12N15/62C12N15/11C12N1/21C12N5/10C07K14/705A61K38/17A61P35/00G01N33/68G01N33/574C12R1/19
CPCC07K14/705C12N15/85A61P35/00G01N33/6893G01N33/57411G01N33/57442G01N33/57415G01N33/57434G01N33/57484C12N2800/107C07K2319/21C07K2319/30A61K38/00G01N2333/705
Inventor 余乐程威盛鑫龙刘佩佩
Owner WUHAN AIBO TAIKE BIOTECH CO LTD