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Jig for cell or tissue cryopreservation

A cryopreservation and fixture technology, which is applied in the preservation of human or animal bodies, tissue culture, animal cells, etc., can solve the problem of difficult operation and achieve the effect of excellent insertion operability

Active Publication Date: 2022-02-18
MITSUBISHI PAPER MILLS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that in this method, eggs and embryos are placed on the film together with a small amount of preservation solution by the operator. This method has the problem of high operational difficulty, but it is possible to freeze eggs or embryos with a high survival rate

Method used

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  • Jig for cell or tissue cryopreservation
  • Jig for cell or tissue cryopreservation
  • Jig for cell or tissue cryopreservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] according to figure 1 and image 3 The cryopreservation jig of Example 1 was produced in the manner shown. With polyethylene terephthalate resin film (total light transmittance 89%, haze value 2.3%) as the support body, use polytetrafluoroethylene porous material (pore diameter 0.2μm, porosity 71 %, thickness 35 μm) as the storage liquid absorber, using HenkelJapan company's hot-melt polyurethane resin Purmelt (registered trademark) QR 170-7141P as the adhesive layer, only the two ends of the short axis side are bonded to the support body Fix it on the top, and make the front end with the mounting part. In addition, ABS resin is used to make the grip part of the main body part, the fitting structure part, and the cover part. The front end part, the grasping part, and the fitting structure part were joined together, and the jig for cryopreservation of Example 1 was produced. The body member of the jig for cryopreservation of Example 1 has a front end portion whose le...

Embodiment 2

[0111] The cryopreservation jig of Example 2 was produced in the same manner as in Example 1 except that the length in the short-axis direction of the front end portion of the main body member was changed from 1.75 mm to 1.5 mm.

Embodiment 3

[0113] The cryopreservation jig of Example 3 was produced in the same manner as in Example 2 except that the length in the longitudinal direction of the inner cavity of the lid member was changed from 35 mm to 40 mm.

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Abstract

The purpose of the present invention is to provide a jig for cryopreservation that achieves excellent workability in a freezing operation and a thawing operation for cells or tissues. The present invention is directed to a jig for cell or tissue cryopreservation at least comprising a body member having a strip-shaped tip section, and a cap member that encloses the tip section of the main body member, wherein the tip section has at least a placement part having a preservative solution absorbent, the short axial length of the tip section is greater than or equal to 70% of the inner diameter of the cap member, and the long axis length of an inner cavity of the cap member is less than or equal to 200% of the long axis length of the tip section.

Description

technical field [0001] The present invention relates to a jig for cryopreservation of cells or tissues. Background technique [0002] Various industrial fields are looking for excellent cell or tissue preservation technology. For example, in the bovine embryo transfer technology, the embryos are cryopreserved, and the embryos are thawed and transplanted according to the estrous cycle of the recipient cattle. In addition, in the treatment of human infertility, eggs or ovaries are collected from the mother, cryopreserved in order to adapt to the timing of transplantation, and thawed for use at the time of transplantation. [0003] Generally, long-term culture of cells or tissues in vitro is not preferable because cells or tissues collected from a living body gradually lose activity or change in properties even in a culture medium. Therefore, techniques for long-term preservation while maintaining biological activity are important. With superior preservation techniques, coll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0263A01N1/0273A01N1/0268A61D19/024C12M45/22A61D17/002C12N2523/00C12M23/04C12N5/0608A01N1/021
Inventor 松泽笃史吉田翔
Owner MITSUBISHI PAPER MILLS LTD