Preparation method of combined extraction of alliin and allicin

Abstract

The present application relates to the technical field of extraction methods for natural active substances, and specifically discloses a preparation method for combined extraction of alliin and allicin. The combined extraction and preparation method of alliin and allicin includes the steps of raw material pretreatment, alliin extraction, allicin preparation, allicin extraction and the like; the garlic grains are pretreated by using an enzyme activity inhibitor to inhibit the enzymes in the garlic grains. The occurrence of the solution reaction, and then alliinase is extracted by Na / K phosphate buffer, so that alliinase and alliin are separated, and alliin can be directly purified into a product, and can also be mixed with Na / K phosphoric acid extract. The alliinase undergoes enzymatic hydrolysis to prepare high-purity allicin. The production line designed in this application can simultaneously produce two high-value products, alliin and allicin, and the products have high extraction rate and purity and good quality.

Description

technical field [0001] The present application relates to the technical field of methods for extracting natural active substances, and more particularly, to a method for the joint extraction and preparation of alliin and allicin. Background technique [0002] Allicin (Allicin) is an organosulfur compound extracted from the Alliaceae Allium plant. Biochemical analysis proved that fresh garlic does not contain allicin, but its precursor - alliin. Alliin exists in garlic in an unstable and odorless form. After garlic is destroyed, alliinase in garlic is activated to catalyze the decomposition of alliin into allicin. [0003] At present, the common extraction methods of allicin are generally as follows: [0004] First, the steam distillation method has simple equipment and low cost, but the disadvantage of this method is that the temperature of the extraction conditions is high, which can easily cause the volatilization loss of allicin, so the content of allicin in the extract...

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Problems solved by technology

[0004] The first one, the steam distillation method has simple equipment and low cost, but the disadvantage of this method is that the temperature of the extraction condition is high, which can easily cause the volatilization loss of allicin, so the content of allicin in the extracted species is only 5% about;

[0005] The second method, organic solvent extraction, often uses solvents such as methanol, acetone, and ether as the extraction solution, but the disadvantage of this method is that the amount of organic solvent is large, and it is easy to remain, which affects the quality of the product, and some solvents are more toxic and are not suitable for food field

[0006] The applicant believes that the above-mentioned relevant extraction methods have the following defects: first, the extraction rate and purity of allicin need to be improved; second, the production line of the above-mentioned method can only extract allicin, and the parts that cannot be extracted have high added value of alliin, resulting in waste of alliin