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Combined extraction preparation method of alliin and allicin

A technology of combined extraction and alliin, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of large amount of organic solvent, easy residue, and inability to extract alliin

Active Publication Date: 2022-03-04
无锡江大百泰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first one, the steam distillation method has simple equipment and low cost, but the disadvantage of this method is that the temperature of the extraction condition is high, which can easily cause the volatilization loss of allicin, so the content of allicin in the extracted species is only 5% about;
[0005] The second method, organic solvent extraction, often uses solvents such as methanol, acetone, and ether as the extraction solution, but the disadvantage of this method is that the amount of organic solvent is large, and it is easy to remain, which affects the quality of the product, and some solvents are more toxic and are not suitable for food field
[0006] The applicant believes that the above-mentioned relevant extraction methods have the following defects: first, the extraction rate and purity of allicin need to be improved; second, the production line of the above-mentioned method can only extract allicin, and the parts that cannot be extracted have high added value of alliin, resulting in waste of alliin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method for the joint extraction of alliin and allicin is carried out according to the following process steps:

[0046] Raw material pretreatment:

[0047]Inject 100kg of water into the pool, drop into 0.5kg of hydroxylamine hydrochloride, stir and mix; at a stirring rate of 100rpm, continuously inject glacial acetic acid into the pool until the pH value of the solution in the pool is 3.5, and obtain the enzyme activity inhibitor;

[0048] Peel off the outer skin of the garlic, wash and remove; weigh 50kg of intact and clean garlic grains, put them in the enzyme inhibitor, and soak them at 20°C for 6 hours; after soaking, transfer the garlic grains and enzyme inhibitors to the grinder , the pulverizer is equipped with a cooling jacket, so that the temperature in the pulverizer is controlled at 1°C, and the pulverizer pulverizes the homogenate to obtain the raw material of mashed garlic;

[0049] Add 50kgNa / K phosphate buffer solution to the garlic raw m...

Embodiment 2-8

[0062] The difference between the preparation method of alliin and allicin combined extraction and that of Example 1 is that the process parameters in the raw material pretreatment step are different, and the specific process parameters are shown in the following table.

[0063] Table 1. Process parameters in raw material pretreatment step

[0064] parameter Example 1 Example 2 Example 3 Example 4 Enzyme inhibitor pH value 3.5 5 4 4 Enzyme activity inhibitor soaking time / h 6 6 6 6 Breaking temperature / ℃ 1 1 1 15 broken environment air atmosphere air atmosphere air atmosphere air atmosphere Extraction temperature / ℃ 1 1 1 15 parameter Example 5 Example 6 Example 7 Example 8 Enzyme inhibitor pH value 4 4 4 4 Enzyme activity inhibitor soaking time / h 6 6 6 12 Breaking temperature / ℃ 4 6 6 6 broken environment air atmosphere air atmosphere Nitrogen atmosphere Nitrogen a...

Embodiment 9

[0066] The difference between the preparation method of combined extraction of alliin and allicin and that of Example 8 is that in the raw material pretreatment step, 0.5 kg of glycerol is first added to 50 kg Na / K phosphate buffer solution, and the raw material of mashed garlic is extracted after mixing evenly.

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PUM

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Abstract

The invention relates to the technical field of extraction methods of natural active substances, and particularly discloses a combined extraction preparation method of alliin and allicin. The invention discloses a combined extraction preparation method of alliin and allicin. The combined extraction preparation method comprises the steps of raw material pretreatment, alliin extraction, allicin preparation, allicin extraction and the like. According to the method, garlic granules are pretreated by using an enzyme activity inhibitor to inhibit the occurrence of an enzymolysis reaction in the garlic granules, then alliinase is extracted by using a Na / K phosphoric acid buffer solution, so that the alliinase is separated from alliin, and the alliin can be directly purified into a product and can also be subjected to an enzymolysis reaction with alliinase in the Na / K phosphoric acid extract solution to prepare high-purity allicin. The production line designed by the invention can be used for simultaneously preparing two high-value products, namely alliin and allicin, and the products are relatively high in extraction rate and purity and good in quality.

Description

technical field [0001] This application relates to the technical field of extraction methods of natural active substances, more specifically, it relates to a preparation method for the joint extraction of alliin and allicin. Background technique [0002] Allicin (Allicin) is an organosulfur compound extracted from the Allium genus of the Allium family. Its scientific name is diallyl thiosulfinate. It has a strong garlic smell and is volatile when heated. Biochemical analysis proves that fresh garlic does not contain allicin, but its precursor - alliin. Alliin exists in garlic in an unstable and odorless form. After the garlic is destroyed, alliinase in garlic is activated to catalyze the decomposition and synthesis of alliin into allicin. [0003] At present, the common extraction methods of allicin are generally as follows: [0004] The first one, the steam distillation method has simple equipment and low cost, but the disadvantage of this method is that the temperature o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C319/28C07C321/18C07C323/65C12P11/00
CPCC07C317/48C07C319/28C12P11/00C07C321/18C07C323/65
Inventor 陈国安杨盛荣张显久赵樾
Owner 无锡江大百泰科技有限公司
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