Construction method and application of non-alcoholic fatty liver animal model based on NLRP3 gene knockout

Abstract

The invention provides a construction method and application of a non-alcoholic fatty liver animal model based on NLRP3 gene knockout. According to the animal model construction method, an NLRP3 gene knockout animal (NLRP3 <- / ->) is taken as a model animal, a non-alcoholic fatty liver model is constructed through high-fat and high-fructose diet, the expression levels of NLRP3 and related factors are detected, the correlation between each index and the non-alcoholic fatty liver is analyzed, and the effect of NLRP3 in the pathogenesis of the non-alcoholic fatty liver is elaborated. The model lays a research foundation for further development of drug effect observation and related molecular mechanism research of fatty liver.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a construction method and application of a non-alcoholic fatty liver animal model based on NLRP3 gene knockout. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a chronic hepatic lipid metabolism syndrome caused by no history of excessive drinking and other definite liver damage factors. It is caused by various factors such as insulin resistance and lipid metabolism disorders. of liver damage. The early pathological form of NAFLD is simple hepatic steatosis. With the development of the disease and the participation of non-alcoholic steatohepatitis (NASH), it gradually develops into liver fibrosis and cirrhosis, and finally leads to liver failure. According to epidemiological surveys, the prevalence of NAFLD in normal population in western countries is about 20%-40%. With the rapid development of my country's economy, people's living habits...

AI Technical Summary

Problems solved by technology

The pathogenic mechanism of the nutritional fatty liver model is similar to that of human NAFLD, and it has the characteristics of gradual development. The disadvantage of this method is that the modeling time is long, and the modeling period is generally 2-3 months; The modeling time is short and the lesions are obvious, but its pathogenesis and liver pathological changes are quite different from human NAFLD, and the drug toxicity is strong, resulting in high mortality in model animals; gene knockout or gene mutation models can spontaneously form fatty liver , and accompanied by metabolic syndrome such as fat metabolism disorder, can reveal the occurrence and development mechanism of non-alcoholic fatty liver from the overall level, tissue and organ level, cell and molecular level

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